Calcein AM Assay Kit ab228556 is a simple, extremely sensitive quantitative assay to measure the cell viability of adherent and suspension cells.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Cellular and molecular biology (Noisy-le-Grand, France) 68:28-322022Hypoxia disturbs the Migration and Adhesion characteristics of Mesenchymal Stem Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRamada R Khasawneh,Ejlal Abu-El-RubPubMed 37114312
- Materials today. Bio 16:1004342022Aptamer engineering exosomes loaded on biomimetic periosteum to promote angiogenesis and bone regeneration by targeting injured nerves via JNK3 MAPK pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanlin Su,Qing Gao,Rongli Deng,Lian Zeng,Jingyi Guo,Bing Ye,Jialin Yu,Xiaodong GuoPubMed 36186848
Key facts
- Detection method
Fluorescent
- Sample types
Suspension cells, Adherent cells
- Assay type
Cell-based (quantitative)
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Calcein AM Assay Kit ab228556 is a simple, extremely sensitive quantitative assay to measure the cell viability of adherent and suspension cells.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
Fluorescent
- Sample types
Suspension cells, Adherent cells
- Assay type
Cell-based (quantitative)
- Assay Platform
Microplate reader
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Calcein AM Assay Kit ab228556 is a simple, extremely sensitive quantitative assay to measure the cell viability of adherent and suspension cells. It can detect as low as 50 viable cells in less than 30 minutes. Calcein AM is a non-fluorescent, hydrophobic compound that easily penetrates intact and live cells. During the Calcein AM assay, hydrolysis of Calcein AM by intracellular esterases produces a hydrophilic, strongly fluorescent compound that is retained in the cell cytoplasm and can be measured at Ex/Em = 485/530 nm. The measured fluorescence intensity is proportional to the number of viable cells. The Calcein AM assay can be used for cell proliferation, cell viability, and cytotoxicity studies. Review our to learn more about our other , and assay kits.
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Functional Studies - Calcein AM Assay Kit (Fluorometric) (ab228556)
Cell Viability Assay: Fibroblast cells were grown in DMEM supplemented with 10% FBS, harvested using trypsin and counted using Trypan blue and a hemocytometer. Cells were serially diluted in a clear cell culture plate and incubated for 30 minutes with Calcein AM at 37°C. After incubation, cells were lysed using Cell Lysis Buffer for 10 minutes at room temperature. Cell lysates were transferred into a 96-well white plate and fluorescence was measured. Inset graph is an expanded segment of the assay data at lower cell number per well.
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