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AB228556

Calcein AM Assay Kit (Fluorometric)

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(4 Publications)

Calcein AM Assay Kit (ab228556) is a sensitive quantitative assay to measure the cell viability of adherent and suspension cells.

- Fluorescent readout - use with microplate reader
- Detect as low as 50 viable cells in less than 30 min
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Functional Studies - Calcein AM Assay Kit (Fluorometric) (AB228556)
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Supplier Data

Functional Studies - Calcein AM Assay Kit (Fluorometric) (AB228556)

Cell Viability Assay : Fibroblast cells were grown in DMEM supplemented with 10% FBS, harvested using trypsin and counted using Trypan blue and a hemocytometer. Cells were serially diluted in a clear cell culture plate and incubated for 30 minutes with Calcein AM at 37°C. After incubation, cells were lysed using Cell Lysis Buffer for 10 minutes at room temperature. Cell lysates were transferred into a 96-well white plate and fluorescence was measured. Inset graph is an expanded segment of the assay data at lower cell number per well.

Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based

Results type

Quantitative

Assay Platform

Microplate reader

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Product details

Calcein AM Assay Kit (ab228556) can detect as low as 50 viable cells in less than 30 minutes.

How the Calcein AM assay works

Calcein AM is a non-fluorescent, hydrophobic compound that easily penetrates intact and live cells. During the Calcein AM assay, hydrolysis of Calcein AM by intracellular esterases produces a hydrophilic, strongly fluorescent compound that is retained in the cell cytoplasm and can be measured at Ex/Em = 485/530 nm. The measured fluorescence intensity is proportional to the number of viable cells.

The Calcein AM assay can be used for cell proliferation, cell viability, and cytotoxicity studies.

Calcein AM assay protocol summary

- prepare cells

- prepare Cell Dye II/Calcein AM solution

- add prepared Cell Dye II/Calcein AM solution to each well

- incubate at 37ºC for 30 minutes

- read fluorescence (Ex/Em = 485/530 nm)

Review the to learn about more kits to perform a , and .

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "1000Test": { "sellingSize": "1000 Test", "publicAssetCode":"ab228556-1000Test", "assetComponentDetails": [ { "size":"1 x 100 mL", "name":"Assay Buffer 27", "number":"AB228556-CMP02", "productcode":"AB323965" }, { "size":"2 x 1 Each", "name":"Cell Dye II", "number":"AB228556-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

See full target information Calcein,AM
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cancer letters 612:217468 PubMed39826669

2025

Targeting dormant disseminated tumor cells and their permissive niche by pro-resolving mediators derived from resolution-phase macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Odelya Gilon-Zaltsman,Keren Weidenfeld-Barenboim,Hadeel Samara,Yonatan Feuermann,Shira Michaeli-Ashkenasi,Sagie Schif-Zuck,Palle Von Huth,Sergei Butenko,Simaan Assi,Edmond Sabo,Amiram Ariel,Dalit Barkan

Journal of biochemical and molecular toxicology 38:e23808 PubMed39132830

2024

Calycosin activates Nrf2/Keap1 signaling to ameliorate hydrogen peroxide-induced spinal cord neuron death and mitochondrial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Ruan,Ziming Cai,Zhengwen Kang,Jinzhu Liang,He Tian,Qinghe Yu,Qiusheng Zhang,Wenping Lin

Cellular and molecular biology (Noisy-le-Grand, France) 68:28-32 PubMed37114312

2022

Hypoxia disturbs the Migration and Adhesion characteristics of Mesenchymal Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ramada R Khasawneh,Ejlal Abu-El-Rub

Materials today. Bio 16:100434 PubMed36186848

2022

Aptamer engineering exosomes loaded on biomimetic periosteum to promote angiogenesis and bone regeneration by targeting injured nerves via JNK3 MAPK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yanlin Su,Qing Gao,Rongli Deng,Lian Zeng,Jingyi Guo,Bing Ye,Jialin Yu,Xiaodong Guo
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Product promise

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