Calcium Assay Kit (Colorimetric) ab102505 provides a simple assay to determine calcium concentration.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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Publications
- Nature communications 15:36842024Development of Aptamer-DNAzyme based metal-nucleic acid frameworks for gastric cancer therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiaqi Yan,Rajendra Bhadane,Meixin Ran,Xiaodong Ma,Yuanqiang Li,Dongdong Zheng,Outi M H Salo-Ahen,Hongbo ZhangPubMed 38693181
- The Journal of clinical investigation 134:2024BCMA- and CST6-specific CAR T cells lyse multiple myeloma cells and suppress murine osteolytic lesions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFumou Sun,Yan Cheng,Jin-Ran Chen,Visanu Wanchai,David E Mery,Hongwei Xu,Dongzheng Gai,Samer Al Hadidi,Carolina Schinke,Sharmilan Thanendrarajan,Maurizio Zangari,Frits van Rhee,Guido Tricot,John D Shaughnessy,Fenghuang ZhanPubMed 37883186
Key facts
- Sample types
Urine, Plasma, Tissue Extracts, Serum, Other biological fluids, Cell Lysate
- Assay type
Quantitative
- Reactive species
Mammals
- Range
0.2 - 1 mM
- Assay time
20m
- Sensitivity
> 0.1 mM
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Target data
Alternative names
Ca++, Ca2+
What's included?
Recommended products
Calcium Assay Kit (Colorimetric) ab102505 provides a simple assay to determine calcium concentration.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Alternative names
Ca++, Ca2+
Key facts
- Sample types
Urine, Plasma, Tissue Extracts, Serum, Other biological fluids, Cell Lysate
- Assay type
Quantitative
- Reactive species
Mammals
- Range
0.2 - 1 mM
- Assay time
20m
- Assay Platform
Microplate reader
- Sensitivity
> 0.1 mM
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Calcium Assay Kit (Colorimetric) ab102505 provides a simple assay to determine calcium concentration within the physiological range of 0.4 – 100 mg/dL (0.2 – 1 mM).
In the calcium assay protocol, a chromogenic complex is formed between calcium ions and 0-cresolphthalein. The complex is measured at OD = 575 nm.
This calcium assay detects free calcium ions only.
Calcium assay protocol summary:
- add samples and standards to wells
- add chromogenic reagent, add calcium assay buffer and incubate for 5-10 min at room temp
- analyze with microplate reader
This product is manufactured by BioVision, an Abcam company and was previously called K380 Calcium Colorimetric Assay Kit. K380-250 is the same size as the 250 test size of ab102505.
Supplementary info
- Activity summary
Calcium often referred to as Ca2+ acts mechanically as an important signaling molecule and structural element in biological systems. It is a small ion with an atomic mass of 40.08 Daltons which allows it to easily move across membranes. You can find calcium expressed in various tissues prominently within bones teeth and intracellular compartments such as the endoplasmic reticulum and mitochondria. As an ion calcium plays an integral role in cellular signaling processes enabling numerous functions including muscle contraction and neurotransmitter release.
- Biological function summary
Calcium modulates various cellular activities serving as a messenger in signal transduction pathways. It participates in forming complexes with proteins like calmodulin and troponin which subsequently activate an array of cellular processes. Calcium is also pivotal in maintaining the structural integrity of bones through its deposition as hydroxyapatite. Calcium ions influence cellular excitability and are involved in numerous physiological processes through their interaction with various ion channels enzymes and proteins.
- Pathways
Calcium plays critical roles in several including the phosphoinositide signaling pathway and the muscle contraction pathway. In these pathways calcium influx and release modulate activities of proteins like calmodulin and phospholipase C. Calcium’s interactions facilitate the propagation of intracellular signals impacting cell proliferation differentiation and programmed cell death. These pathways highlight calcium’s role in maintaining cellular homeostasis and function.
- Associated diseases and disorders
Calcium imbalance influences conditions such as osteoporosis and cardiac arrhythmias. Dysregulation of calcium homeostasis affects bone density leading to osteoporosis a disorder characterized by increased bone fragility. Furthermore abnormal calcium handling in cardiac myocytes contributes to arrhythmias as calcium ions regulate cardiac muscle excitability and contractility. Proteins like calmodulin and troponin linked with calcium play roles in these conditions highlighting the clinical impact of calcium signaling disturbances.
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4 product images
G?r?gh, Tibor et al., BMC musculoskeletal disorders?vol. 18,1 500., Fig 3, doi:10.1186/s12891-017-1860-2Functional Studies - Calcium Assay Kit (Colorimetric) (ab102505)
Time course of mineralization. Measurement of LDH activity. After a 15-day cultivation period of each of 2 x 106 osteoblasts growing in OSC-supplemented media (OSC+) and control osteoblasts growing in non-OSC-supplemented media (OSC-) the LDH activity in the cell free supernatants was determined as described in Material and Methods. Osteoblasts treated with 1% (v/v) Triton-X100 for 1 hour (c) served as a second control. The experiments were carried out with three primary cultures in triplicate
Koskela, A et al., Scientific reports?vol. 7,1 6841., Fig 4, doi:10.1038/s41598-017-07359-6Functional Studies - Calcium Assay Kit (Colorimetric) (ab102505)
Effect of PFOA on calcium concentrations in cultured human osteoblasts after exposure for 3 or 5 weeks. Plots represent mean ± SD.
Functional Studies - Calcium Assay Kit (Colorimetric) (ab102505)
Ca2+ concentration (millimolar) in various biologicals. Shown bars are average of values from dilutions neat to 1:8.
Functional Studies - Calcium Assay Kit (Colorimetric) (ab102505)
Example of standard curve obtained using ab102505
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