Calcium Assay Kit (Colorimetric) ab102505 is a quantitative, mix-and-read assay with just one 10-min incubation step. Readout on any colorimetric (575 nm) plate reader.
- Cited in over 100 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Nature communications 15:36842024Development of Aptamer-DNAzyme based metal-nucleic acid frameworks for gastric cancer therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiaqi Yan,Rajendra Bhadane,Meixin Ran,Xiaodong Ma,Yuanqiang Li,Dongdong Zheng,Outi M H Salo-Ahen,Hongbo ZhangPubMed 38693181
- The Journal of clinical investigation 134:2024BCMA- and CST6-specific CAR T cells lyse multiple myeloma cells and suppress murine osteolytic lesions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFumou Sun,Yan Cheng,Jin-Ran Chen,Visanu Wanchai,David E Mery,Hongwei Xu,Dongzheng Gai,Samer Al Hadidi,Carolina Schinke,Sharmilan Thanendrarajan,Maurizio Zangari,Frits van Rhee,Guido Tricot,John D Shaughnessy,Fenghuang ZhanPubMed 37883186
- Frontiers in immunology 14:12721912023Wound healing and Cadmium detoxification in the earthworm - a potential case for coelomocytes?Applications:Unspecified applicationReactive species:Unspecified reactive speciesGerhard P Aigner,Veronika Peer,Birgit Fiechtner,Cláudio Adriano Piechnik,Martina HöcknerPubMed 38116011
- Nature communications 14:62422023Distinct and targetable role of calcium-sensing receptor in leukaemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRaquel S Pereira,Rahul Kumar,Alessia Cais,Lara Paulini,Alisa Kahler,Jimena Bravo,Valentina R Minciacchi,Theresa Krack,Eric Kowarz,Costanza Zanetti,Parimala Sonika Godavarthy,Fabian Hoeller,Pablo Llavona,Tabea Stark,Georg Tascher,Daniel Nowak,Eshwar Meduri,Brian J P Huntly,Christian Münch,Francesco Pampaloni,Rolf Marschalek,Daniela S KrausePubMed 37802982
- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e22076022023Sympathetic Innervation Regulates Osteocyte-Mediated Cortical Bone Resorption during Lactation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiaoyue Guo,Ningrong Chen,Cheng Qian,Cheng Qi,Kathleen Noller,Mei Wan,Xiaonan Liu,Weixin Zhang,Patrick Cahan,Xu CaoPubMed 37186379
- Toxins 15:2023Harzianic Acid Activity against and Its Role in Calcium Regulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlessia Staropoli,Paola Cuomo,Maria Michela Salvatore,Gaetano De Tommaso,Mauro Iuliano,Anna Andolfi,Gian Carlo Tenore,Rosanna Capparelli,Francesco VinalePubMed 37104175
- Brain, behavior, and immunity 110:348-3642023Schwann cell insulin-like growth factor receptor type-1 mediates metastatic bone cancer pain in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLorenzo Landini,Matilde Marini,Daniel Souza Monteiro de Araujo,Antonia Romitelli,Marco Montini,Valentina Albanese,Mustafa Titiz,Alessandro Innocenti,Francesca Bianchini,Pierangelo Geppetti,Romina Nassini,Francesco De LoguPubMed 36940752
- Nature communications 14:9062023Sugar transporter Slc37a2 regulates bone metabolism in mice via a tubular lysosomal network in osteoclasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPei Ying Ng,Amy B P Ribet,Qiang Guo,Benjamin H Mullin,Jamie W Y Tan,Euphemie Landao-Bassonga,Sébastien Stephens,Kai Chen,Jinbo Yuan,Laila Abudulai,Maike Bollen,Edward T T T Nguyen,Jasreen Kular,John M Papadimitriou,Kent Søe,Rohan D Teasdale,Jiake Xu,Robert G Parton,Hiroshi Takayanagi,Nathan J PavlosPubMed 36810735
- International journal of molecular sciences 24:20236-Shogaol Overcomes Gefitinib Resistance via ER Stress in Ovarian Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTae Woo Kim,Hee Gu LeePubMed 36768961
- Brain communications 5:fcac3402023Multiple pathways of lipid dysregulation in amyotrophic lateral sclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatherine Phan,Ying He,Surabhi Bhatia,Russell Pickford,Gordon McDonald,Srestha Mazumder,Hannah C Timmins,John R Hodges,Olivier Piguet,Nicolas Dzamko,Glenda M Halliday,Matthew C Kiernan,Woojin Scott KimPubMed 36632187
Key facts
- Sample types
- Urine, Plasma, Tissue Extracts, Serum, Other biological fluids, Cell Lysate
- Assay type
- Quantitative
- Reactive species
- Mammals
- Range
- 0.2 - 1 mM
- Assay time
- 20m
- Sensitivity
- > 0.1 mM
Associated Products
Select an associated product type
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Target data
Alternative names
Ca++, Ca2+
What's included?
Recommended products
Calcium Assay Kit (Colorimetric) ab102505 is a quantitative, mix-and-read assay with just one 10-min incubation step. Readout on any colorimetric (575 nm) plate reader.
- Cited in over 100 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Sample types
- Urine, Plasma, Tissue Extracts, Serum, Other biological fluids, Cell Lysate
- Assay type
- Quantitative
- Reactive species
- Mammals
- Range
- 0.2 - 1 mM
- Assay time
- 20m
- Assay Platform
- Microplate reader
- Sensitivity
- > 0.1 mM
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Calcium Assay Kit (Colorimetric) ab102505 provides a simple assay to determine calcium concentration within the physiological range of 0.4 – 100 mg/dL (0.2 – 1 mM).
How the assay works
In the calcium assay protocol, a chromogenic complex is formed between calcium ions and 0-cresolphthalein. The complex is measured at OD = 575 nm.
Calcium assay protocol summary
- - Add samples and standards to wells
- - Add chromogenic reagent, add calcium assay buffer and incubate for 5-10 min at room temp
- - Analyze with microplate reader
Related Calcium assay products
Calcium assay kit ab102505 is designed for use with liquid samples such as biological fluids, cell lysates, and tissue extracts.
For the analysis of calcium flux in cell cultures in imaging assays, we supply Fluo-8 Calcium Flux Assay Kits. Calcium Flux Assay Kit (Fluo-8, No Wash) Calcium Flux Assay Kit (Fluo-8, No Wash) ab112129 is generally preferred as the workflow is simpler. The alternative is Medium removal kit Fluo-8 Calcium Flux Assay Kit Fluo-8 Calcium Assay Kit - Medium Removal ab112128. Note - calcium flus assays are also referred to as calcium influx assays.
For the analysis of calcium flux in cultured cells in a flow cytometer, we supply Calcium Flux Assay Kit (Flow cytometry) (Calcium Flux Assay Kit (Flow cytometry) ab233472).
For the imaging of calcium deposits in paraffin sections, we supply Von Kossa (Calcium Stain) Kit Von Kossa Stain Kit (Calcium Stain) ab150687.
Getting the best performance from ab102505
This calcium assay detects free calcium ions only.
Other notes
This product is manufactured by BioVision, an Abcam company and was previously called K380 Calcium Colorimetric Assay Kit. K380-250 is the same size as the 250 test size of ab102505.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Calcium often referred to as Ca2+ acts mechanically as an important signaling molecule and structural element in biological systems. It is a small ion with an atomic mass of 40.08 Daltons which allows it to easily move across membranes. You can find calcium expressed in various tissues prominently within bones teeth and intracellular compartments such as the endoplasmic reticulum and mitochondria. As an ion calcium plays an integral role in cellular signaling processes enabling numerous functions including muscle contraction and neurotransmitter release.
- Biological function summary
Calcium modulates various cellular activities serving as a messenger in signal transduction pathways. It participates in forming complexes with proteins like calmodulin and troponin which subsequently activate an array of cellular processes. Calcium is also pivotal in maintaining the structural integrity of bones through its deposition as hydroxyapatite. Calcium ions influence cellular excitability and are involved in numerous physiological processes through their interaction with various ion channels enzymes and proteins.
- Pathways
Calcium plays critical roles in several including the phosphoinositide signaling pathway and the muscle contraction pathway. In these pathways calcium influx and release modulate activities of proteins like calmodulin and phospholipase C. Calcium’s interactions facilitate the propagation of intracellular signals impacting cell proliferation differentiation and programmed cell death. These pathways highlight calcium’s role in maintaining cellular homeostasis and function.
- Associated diseases and disorders
Calcium imbalance influences conditions such as osteoporosis and cardiac arrhythmias. Dysregulation of calcium homeostasis affects bone density leading to osteoporosis a disorder characterized by increased bone fragility. Furthermore abnormal calcium handling in cardiac myocytes contributes to arrhythmias as calcium ions regulate cardiac muscle excitability and contractility. Proteins like calmodulin and troponin linked with calcium play roles in these conditions highlighting the clinical impact of calcium signaling disturbances.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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5 product images
Functional Studies - Calcium Assay Kit (Colorimetric) (ab102505)
Ca2+ concentration (millimolar) in various biologicals. Shown bars are average of values from dilutions neat to 1:8.
Görögh, Tibor et al., BMC musculoskeletal disorders-vol. 18,1 500., Fig 3, doi:10.1186/s12891-017-1860-2Functional Studies - Calcium Assay Kit (Colorimetric) (ab102505)
Time course of mineralization. Measurement of LDH activity. After a 15-day cultivation period of each of 2 x 106 osteoblasts growing in OSC-supplemented media (OSC+) and control osteoblasts growing in non-OSC-supplemented media (OSC-) the LDH activity in the cell free supernatants was determined as described in Material and Methods. Osteoblasts treated with 1% (v/v) Triton-X100 for 1 hour (c) served as a second control. The experiments were carried out with three primary cultures in triplicate
Koskela, A et al., Scientific reports: vol. 7,1 6841., Fig 4, doi:10.1038/s41598-017-07359-6Functional Studies - Calcium Assay Kit (Colorimetric) (ab102505)
Effect of PFOA on calcium concentrations in cultured human osteoblasts after exposure for 3 or 5 weeks. Plots represent mean ± SD.
Functional Studies - Calcium Assay Kit (Colorimetric) (ab102505)
Example of standard curve obtained using ab102505
Biochemical assay - Calcium Assay Kit (Colorimetric) (ab102505)
Diagram showing the principles of the Calcium assay method.
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