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AB102505

Calcium Assay Kit (Colorimetric)

5

(4 Reviews)

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(151 Publications)

Calcium Assay Kit (Colorimetric) ab102505 is a quantitative, mix-and-read assay with just one 10-min incubation step. Readout on any colorimetric (575 nm) plate reader.

- BIOVISION® assay kit
- Cited in over 100 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
6 Images
Functional Studies - Calcium Assay Kit (Colorimetric) (AB102505)
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Lab

Functional Studies - Calcium Assay Kit (Colorimetric) (AB102505)

Ca2+ concentration (millimolar) in various biologicals. Shown bars are average of values from dilutions neat to 1 : 8.

Functional Studies - Calcium Assay Kit (Colorimetric) (AB102505)
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Supplier Data

Functional Studies - Calcium Assay Kit (Colorimetric) (AB102505)

Example of standard curve obtained using ab102505

Functional Studies - Calcium Assay Kit (Colorimetric) (AB102505)
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Functional Studies - Calcium Assay Kit (Colorimetric) (AB102505)

Time course of mineralization. Measurement of LDH activity. After a 15-day cultivation period of each of 2 x 106 osteoblasts growing in OSC-supplemented media (OSC+) and control osteoblasts growing in non-OSC-supplemented media (OSC-) the LDH activity in the cell free supernatants was determined as described in Material and Methods. Osteoblasts treated with 1% (v/v) Triton-X100 for 1 hour (c) served as a second control. The experiments were carried out with three primary cultures in triplicate

Görögh, Tibor et al., BMC musculoskeletal disorders-vol. 18,1 500., Fig 3, doi:10.1186/s12891-017-1860-2

Functional Studies - Calcium Assay Kit (Colorimetric) (AB102505)
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Functional Studies - Calcium Assay Kit (Colorimetric) (AB102505)

Effect of PFOA on calcium concentrations in cultured human osteoblasts after exposure for 3 or 5 weeks. Plots represent mean ± SD.

Koskela, A et al., Scientific reports: vol. 7,1 6841., Fig 4, doi:10.1038/s41598-017-07359-6

Biochemical assay - Calcium Assay Kit (Colorimetric) (AB102505)
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Biochemical assay - Calcium Assay Kit (Colorimetric) (AB102505)

Diagram showing the principles of the Calcium assay method.

Other - Calcium Assay Kit (Colorimetric) (AB102505)
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Supplier Data

Other - Calcium Assay Kit (Colorimetric) (AB102505)

Representative image of Calcium Assay Kit (Colorimetric) ab102505

Components shown from left to right :

- Calcium Standard

- Calcium Assay Buffer

- Chromogenic Reagent

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Sample types

Urine, Plasma, Tissue Extracts, Serum, Other biological fluids, Cell Lysate

Results type

Quantitative

Sensitivity

> 0.1 mM

Range

0.2 - 1 mM

Assay time

20m

Assay Platform

Microplate reader

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Secondary Antibodies

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Assay Kits

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Calcium Flux Assay Kit (Fluo-8, No Wash)

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Assay Kits

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Phosphate Assay Kit (Colorimetric)

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Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

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Product details

Calcium Assay Kit (Colorimetric) ab102505 provides a simple assay to determine calcium concentration within the physiological range of 0.4 – 100 mg/dL (0.2 – 1 mM).

How the assay works
In the calcium assay protocol, a chromogenic complex is formed between calcium ions and 0-cresolphthalein. The complex is measured at OD = 575 nm.

Calcium assay protocol summary

  • - Add samples and standards to wells
  • - Add chromogenic reagent, add calcium assay buffer and incubate for 5-10 min at room temp
  • - Analyze with microplate reader

Related Calcium assay products
Calcium assay kit ab102505 is designed for use with liquid samples such as biological fluids, cell lysates, and tissue extracts.

For the analysis of calcium flux in cell cultures in imaging assays, we supply Fluo-8 Calcium Flux Assay Kits. Calcium Flux Assay Kit (Fluo-8, No Wash) ab112129 is generally preferred as the workflow is simpler. The alternative is Medium removal kit Fluo-8 Calcium Flux Assay Kit ab112128. Note - calcium flus assays are also referred to as calcium influx assays.

For the analysis of calcium flux in cultured cells in a flow cytometer, we supply Calcium Flux Assay Kit (Flow cytometry) (ab233472).

For the imaging of calcium deposits in paraffin sections, we supply Von Kossa (Calcium Stain) Kit ab150687.

The Calcium Assay Buffer component is also available under ab182458.

Getting the best performance from ab102505
This calcium assay detects free calcium ions only.

Other notes
This product is manufactured by BioVision, an Abcam company and was previously called K380 Calcium Colorimetric Assay Kit. K380-250 is the same size as the 250 test size of ab102505.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calcium often referred to as Ca2+ acts mechanically as an important signaling molecule and structural element in biological systems. It is a small ion with an atomic mass of 40.08 Daltons which allows it to easily move across membranes. You can find calcium expressed in various tissues prominently within bones teeth and intracellular compartments such as the endoplasmic reticulum and mitochondria. As an ion calcium plays an integral role in cellular signaling processes enabling numerous functions including muscle contraction and neurotransmitter release.
Biological function summary

Calcium modulates various cellular activities serving as a messenger in signal transduction pathways. It participates in forming complexes with proteins like calmodulin and troponin which subsequently activate an array of cellular processes. Calcium is also pivotal in maintaining the structural integrity of bones through its deposition as hydroxyapatite. Calcium ions influence cellular excitability and are involved in numerous physiological processes through their interaction with various ion channels enzymes and proteins.

Pathways

Calcium plays critical roles in several including the phosphoinositide signaling pathway and the muscle contraction pathway. In these pathways calcium influx and release modulate activities of proteins like calmodulin and phospholipase C. Calcium’s interactions facilitate the propagation of intracellular signals impacting cell proliferation differentiation and programmed cell death. These pathways highlight calcium’s role in maintaining cellular homeostasis and function.

Calcium imbalance influences conditions such as osteoporosis and cardiac arrhythmias. Dysregulation of calcium homeostasis affects bone density leading to osteoporosis a disorder characterized by increased bone fragility. Furthermore abnormal calcium handling in cardiac myocytes contributes to arrhythmias as calcium ions regulate cardiac muscle excitability and contractility. Proteins like calmodulin and troponin linked with calcium play roles in these conditions highlighting the clinical impact of calcium signaling disturbances.
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Product protocols

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Target data

See full target information Calcium
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Publications (151)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:22557 PubMed40595199

2025

A pilot clinical trial to explore the effects of UV exposure on vitamin D synthesis and inflammatory responses in vitamin D-Deficient adults.

Applications

Unspecified application

Species

Unspecified reactive species

Chaodeng Mo,Johny Bajgai,Md Habibur Rahman,Soonchul Choe,Sofian Abdul-Nasir,Hui Ma,Wenjing He,Thu Thao Pham,Haiyang Zhang,Goh Seong Hoon,Kim Bomi,Cheol-Su Kim,Kyu-Jae Lee,Insik Shin

Molecular neurodegeneration 20:59 PubMed40389989

2025

Mutations in NEK1 cause ciliary dysfunction as a novel pathogenic mechanism in amyotrophic lateral sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Min-Young Noh,Seong-Il Oh,Young-Eun Kim,Sun Joo Cha,Wonjae Sung,Ki-Wook Oh,Yurim Park,Ji Young Mun,Chang-Seok Ki,Minyeop Nahm,Seung Hyun Kim

PLoS biology 23:e3003164 PubMed40315416

2025

Cadherin-16 regulates acoustic sensory gating in zebrafish through endocrine signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Susannah S Schloss,Zackary Q Marshall,Nicholas J Santistevan,Stefani Gjorcheska,Amanda Stenzel,Lindsey Barske,Jessica C Nelson

Molecular metabolism 97:102158 PubMed40294701

2025

Vitamin D receptor signalling regulates the diet-driven metabolic shift during weaning.

Applications

Unspecified application

Species

Unspecified reactive species

Neha Jawla,Shubhi Khare,Nidhi Yadav,Ranjan Kumar Nanda,G Aneeshkumar Arimbasseri

Cells 14: PubMed40136673

2025

Blockade of HSP70 Improves Vascular Function in a Mouse Model of Type 2 Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Valentina Ochoa Mendoza,Amanda Almeida de Oliveira,Kenia Pedrosa Nunes

Drug design, development and therapy 19:1489-1502 PubMed40051467

2025

Mogrol Regulates the Expression of ATPase Na+/K+ Transport Subunit 3, Inhibits Cardiomyocyte Apoptosis, and Plays a Protective Role Against Myocardial Infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Wang,Jinling Zhou,Weiwei Liu,Wei Wang,Boyan Tian,Jinyu Liu,Han Zhang,Peina He,Xiaoyun Yang,Li Yang,Yueheng Wang

American journal of translational research 17:528-537 PubMed39959245

2025

The molecular mechanism underlying angiogenesis in a mouse model of chronic kidney disease after ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Wang,Liangxiang Lu,Jialun Feng,Jinhua Zheng,Zefeng Wei,Ziqiang Wang

Molecular neurobiology 62:5787-5803 PubMed39630405

2024

EAAT2 Activation Regulates Glutamate Excitotoxicity and Reduces Impulsivity in a Rodent Model of Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Sanjay Das,Kyle Mccloskey,Binod Nepal,Sandhya Kortagere

Nature communications 15:10095 PubMed39572569

2024

Calcium nanoparticles target and activate T cells to enhance anti-tumor function.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Yang,Zhizi Feng,Xinning Lai,Jianwen Li,Zhengwei Cao,Fangchao Jiang,Fanghui Chen,Shuyue Zhan,Feng Kong,Li Yang,Yong Teng,Wendy T Watford,Gang Zhou,Jin Xie

International journal of molecular sciences 25: PubMed39518935

2024

Proteomic Profiling of Endothelial Cell Secretomes After Exposure to Calciprotein Particles Reveals Downregulation of Basement Membrane Assembly and Increased Release of Soluble CD59.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander Stepanov,Daria Shishkova,Victoria Markova,Yulia Markova,Alexey Frolov,Anastasia Lazebnaya,Karina Oshchepkova,Daria Perepletchikova,Daria Smirnova,Liubov Basovich,Egor Repkin,Anton Kutikhin
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Product promise

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