Calcium Assay Kit (Fluorometric) ab112115 provides a simple method for detecting calcium in physiological solutions by using a red fluorescence probe.
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- Aging 13:9542-95652021Gastrodin ameliorates learning and memory impairment in rats with vascular dementia by promoting autophagy flux via inhibition of the Ca/CaMKII signal pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing-Ting Chen,Xue Zhou,Yi-Ni Xu,Yue Li,Xiao-Ying Wu,Quan Xiang,Ling-Yun Fu,Xiao-Xia Hu,Ling Tao,Xiang-Chun ShenPubMed 33714957
- Bioscience reports 39:2019Endoplasmic reticulum Ca2+ release causes Rieske iron-sulfur protein-mediated mitochondrial ROS generation in pulmonary artery smooth muscle cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDapeng Dong,Qiongyu Hao,Ping Zhang,Tao Wang,Fei Han,Xiaodong Liang,Zhenghua FeiPubMed 31710081
- Cell death & disease 10:3412019Baicalein inhibits fibronectin-induced epithelial-mesenchymal transition by decreasing activation and upregulation of calpain-2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Chen et. al.PubMed 31000696
- International journal of molecular sciences 20:2018Titanium Dioxide Nanoparticles Enhance Leakiness and Drug Permeability in Primary Human Hepatic Sinusoidal Endothelial Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Kai Tee et. al.PubMed 30577655
- EBioMedicine 23:173-1842017Neutrophil Membrane Cholesterol Content is a Key Factor in Cystic Fibrosis Lung Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichelle M White et. al.PubMed 28835336
- Biomaterials science 3:45-682015Elastomeric nanocomposite scaffolds made from poly (glycerol sebacate) chemically crosslinked with carbon nanotubes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAkhilesh K Gaharwar,Alpesh Patel,Alireza Dolatshahi-Pirouz,Hongbin Zhang,Kaushik Rangarajan,Giorgio Iviglia,Su-Ryon Shin,Mohammad Asif Hussain,Ali KhademhosseiniPubMed 26146547
Key facts
- Detection method
- Fluorescent
- Sample types
- Plasma, Serum, Cell Lysate
- Assay type
- Quantitative
- Range
- 0.03 - 1 mM
- Assay time
- 30m
- Sensitivity
- = 0.03 mM
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Target data
Alternative names
Ca++, Ca2+
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Calcium Assay Kit (Fluorometric) ab112115 provides a simple method for detecting calcium in physiological solutions by using a red fluorescence probe.
Key facts
- Detection method
- Fluorescent
- Sample types
- Plasma, Serum, Cell Lysate
- Assay type
- Quantitative
- Range
- 0.03 - 1 mM
- Assay time
- 30m
- Assay Platform
- Microplate reader
- Sensitivity
- = 0.03 mM
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Calcium Assay Kit (Fluorometric) ab112115 provides a simple method for detecting calcium in physiological solutions by using a red fluorescence probe. The fluorescence signal can be easily read by a fluorescence microplate reader at Ex/Em = 540/590 nm.
The kit can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. The calcium assay can be completed within 30 minutes.
The calcium assay kit can be used to detect as little as 0.03 mM calcium. The kit has a broad dynamic range (30 μM to 1 mM).
Calcium assay protocol summary:
- add samples and standards to wells
- add reaction mix
- incubate for 5-30 min whilst measuring the fluorescence intensity with a microplate reader
This product is intended to be used *in vitro* with solution-based samples such as cell extracts. If you would like to detect calcium *in vivo* using live cells, please check our , or based assays. Previously called Calcium Quantification Kit.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Calcium ions (Ca²⁺) serve as essential signaling molecules in various cellular processes. These ions have an atomic mass number of 40.08. Calcium ions are highly concentrated in bone tissues teeth and within cells mostly stored in the endoplasmic reticulum and mitochondria. Common indicators of calcium concentration in research include the use of dyes such as Fura-2 and Calbryte 520 AM. These indicators help in visualizing the transient increases in intracellular calcium levels which are important in cellular signaling.
- Biological function summary
Calcium ions mediate various physiological functions including muscle contraction neurotransmitter release and hormone secretion. They typically do not act as lone agents; instead they often function as part of protein complexes particularly with calcium-binding proteins like calmodulin and troponin C. Calcium ions also interact with enzymes such as protein kinases and phosphatases regulating metabolic pathways and genetic expression.
- Pathways
Calcium ions play pivotal roles in the calcium signaling pathway and the cardiac excitation-contraction coupling pathway. In the calcium signaling pathway calcium ions once released from stores like the endoplasmic reticulum activate proteins such as calmodulin which then modulates various cellular activities. In the cardiac excitation-contraction coupling pathway calcium bows its link to proteins like sarcoplasmic reticulum calcium ATPase essential for heart muscle function.
- Associated diseases and disorders
Calcium ion dysregulation can lead to conditions such as osteoporosis and cardiac arrhythmias. In osteoporosis low calcium levels contribute to decreased bone density affecting bone health and making them prone to fractures. In cardiac arrhythmias abnormal calcium handling can result in irregular heartbeats. Proteins such as calbindin-D28k are closely connected to calcium metabolism disorders highlighting the significance of calcium in maintaining physiological balance.
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2 product images
Functional Studies - Calcium Assay kit (Fluorometric) (ab112115)
Ca measured in biologicals showing concentration (mM)
Functional Studies - Calcium Assay kit (Fluorometric) (ab112115)
Standard curve (fluorimetric): mean of duplicates (+/-SD) with background readings subtracted
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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