Calcium Flux Assay Kit (Flow cytometry) (ab233472) is a fluorescence-based assay for detecting intracellular calcium mobilization using a flow cytometer. It can be used for kinetic reading or for endpoint reading.
- Complete kit with buffers, staining dye, and Probenecid to reduce leakage of de-esterified indicators in cells that contain organic aniontransports
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- The Journal of pathology 260:43-552023Oncofetal protein glypican-3 is a biomarker and critical regulator of function for neuroendocrine cells in prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWilliam Butler,Lingfan Xu,Yinglu Zhou,Qing Cheng,J Spencer Hauck,Yiping He,Robert Marek,Zachary Hartman,Liang Cheng,Qing Yang,Mu-En Wang,Ming Chen,Hong Zhang,Andrew J Armstrong,Jiaoti HuangPubMed 36752189
- European cells & materials 41:216-2322021Pulsed electromagnetic fields synergize with graphene to enhance dental pulp stem cell-derived neurogenesis by selectively targeting TRPC1 channels.Applications:Unspecified applicationReactive species:Unspecified reactive speciesT T Madanagopal,Y K Tai,S H Lim,C Hh Fong,T Cao,V Rosa,A Franco-ObregónPubMed 33644848
Key facts
- Detection method
- Fluorescent
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Cell-based (quantitative)
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Alternative names
Ca++, Ca2+
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Calcium Flux Assay Kit (Flow cytometry) (ab233472) is a fluorescence-based assay for detecting intracellular calcium mobilization using a flow cytometer. It can be used for kinetic reading or for endpoint reading.
- Complete kit with buffers, staining dye, and Probenecid to reduce leakage of de-esterified indicators in cells that contain organic aniontransports
Key facts
- Detection method
- Fluorescent
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Cell-based (quantitative)
- Assay Platform
- Flow cytometer
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Calcium Flux Assay Kit (Flow cytometry) (ab233472) is a fluorescence-based assay for detecting intracellular calcium mobilization using a flow cytometer. It can be used for kinetic reading or for endpoint reading
After loading the 520 AM dye into the cells of interest, simply wash the cells and add the calcium flux agonist then read the sample via a flow cytometer using kinetic reading mode or endpoint reading mode at Ex/Em = 490/525 nm.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Calcium ions (Ca²⁺) serve as essential signaling molecules in various cellular processes. These ions have an atomic mass number of 40.08. Calcium ions are highly concentrated in bone tissues teeth and within cells mostly stored in the endoplasmic reticulum and mitochondria. Common indicators of calcium concentration in research include the use of dyes such as Fura-2 and Calbryte 520 AM. These indicators help in visualizing the transient increases in intracellular calcium levels which are important in cellular signaling.
- Biological function summary
Calcium ions mediate various physiological functions including muscle contraction neurotransmitter release and hormone secretion. They typically do not act as lone agents; instead they often function as part of protein complexes particularly with calcium-binding proteins like calmodulin and troponin C. Calcium ions also interact with enzymes such as protein kinases and phosphatases regulating metabolic pathways and genetic expression.
- Pathways
Calcium ions play pivotal roles in the calcium signaling pathway and the cardiac excitation-contraction coupling pathway. In the calcium signaling pathway calcium ions once released from stores like the endoplasmic reticulum activate proteins such as calmodulin which then modulates various cellular activities. In the cardiac excitation-contraction coupling pathway calcium bows its link to proteins like sarcoplasmic reticulum calcium ATPase essential for heart muscle function.
- Associated diseases and disorders
Calcium ion dysregulation can lead to conditions such as osteoporosis and cardiac arrhythmias. In osteoporosis low calcium levels contribute to decreased bone density affecting bone health and making them prone to fractures. In cardiac arrhythmias abnormal calcium handling can result in irregular heartbeats. Proteins such as calbindin-D28k are closely connected to calcium metabolism disorders highlighting the significance of calcium in maintaining physiological balance.
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Flow Cytometry - Calcium Flux Assay Kit (Flow cytometry) (ab233472)
The ATP dose dependent (10 μM, 1 μM or 0 μM ATP) intracellular calcium release was measured by Calcuim Flux Assay Kit (Flow cytometry) (ab233472) in CHO-K1 cells.
Cells were incubated with 520 AM dye for 30 minutes at 37 °C before ATP was added into the cells. The baseline was acquired and the rest of the cells were analyzed after the addition of ATP. The response was measured over time. The analysis was done on NovoCyte™ 3000 Flow Cytometer.
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