Calcium Flux Assay Kit (Fura-2, No Wash, Ratiometric) ab176766 is a no-wash, ratiometric calcium assay that allows homogeneous measurement of intracellular calcium mobilization caused by activation of G-protein-coupled receptors (GPCR) or calcium channels.
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- Theranostics 13:1921-19482023The lysophosphatidic acid-regulated signal transduction network in ovarian cancer cells and its role in actomyosin dynamics, cell migration and entosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaire Ojasalu,Sonja Lieber,Anna M Sokol,Andrea Nist,Thorsten Stiewe,Imke Bullwinkel,Florian Finkernagel,Silke Reinartz,Sabine Müller-Brüsselbach,Robert Grosse,Johannes Graumann,Rolf MüllerPubMed 37064875
- Theranostics 11:1377-13952021Phosphoproteomics identify arachidonic-acid-regulated signal transduction pathways modulating macrophage functions with implications for ovarian cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRaimund Dietze et. al.PubMed 33391540
- Cell cycle (Georgetown, Tex.) 19:3639-36492020Knockdown of miR-1275 protects against cardiomyocytes injury through promoting neuromedin U type 1 receptor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhu Zeng,Haixin Ma,Jing Chen,Nina Huang,Yudan Zhang,Yufei Su,Huifang ZhangPubMed 33323026
- Annals of pediatric endocrinology & metabolism 26:24-302020A cell function study on calcium regulation of a novel calcium-sensing receptor mutation (p.Tyr825Phe).Applications:Unspecified applicationReactive species:Unspecified reactive speciesJung Eun Moon,Hee-Young Yang,Gabbine Wee,Suk-Hyun ParK,Cheol Woo KoPubMed 32871647
- Cell host & microbe 27:556-570.e62020TMEM173 Drives Lethal Coagulation in Sepsis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Zhang et. al.PubMed 32142632
- Cells 9:2020DKK1 Induced by 1,25D3 Is Required for the Mineralization of Osteoblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSungsin Jo,Subin Yoon,So Young Lee,So Yeon Kim,Hyosun Park,Jinil Han,Sung Hoon Choi,Joong-Soo Han,Jae-Hyuk Yang,Tae-Hwan KimPubMed 31963554
- Cell 176:687-701.e52019Small-Molecule Agonists of Ae. aegypti Neuropeptide Y Receptor Block Mosquito Biting.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura B Duvall,Lavoisier Ramos-Espiritu,Kyrollos E Barsoum,J Fraser Glickman,Leslie B VosshallPubMed 30735632
- Cell calcium 74:112-1222018Dysfunction of SERCA pumps as novel mechanism of methylglyoxal cytotoxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPetronela Zizkova et. al.PubMed 30015246
- Biochemical pharmacology 154:293-3022018Selective killing of proinflammatory synovial fibroblasts via activation of transient receptor potential ankyrin (TRPA1).Applications:Unspecified applicationReactive species:Unspecified reactive speciesTorsten Lowin et. al.PubMed 29803505
- Scientific reports 7:56112017Targeting Cellular Calcium Homeostasis to Prevent Cytokine-Mediated Beta Cell Death.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmy L Clark et. al.PubMed 28717166
Key facts
- Detection method
- Fluorescent
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Cell-based
- Reactive species
- Mammals
- Assay time
- 1h 20m
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Target data
Alternative names
Ca++, Ca2+
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Calcium Flux Assay Kit (Fura-2, No Wash, Ratiometric) ab176766 is a no-wash, ratiometric calcium assay that allows homogeneous measurement of intracellular calcium mobilization caused by activation of G-protein-coupled receptors (GPCR) or calcium channels.
Key facts
- Detection method
- Fluorescent
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Cell-based
- Reactive species
- Mammals
- Assay time
- 1h 20m
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Calcium Flux Assay Kit (Fura-2, No Wash, Ratiometric) ab176766 is a no-wash, ratiometric calcium assay that allows homogeneous measurement of intracellular calcium mobilization caused by activation of G-protein-coupled receptors (GPCR) or calcium channels. The assay is suitable for use with 96-well and 384-well microplates.
In the Fura-2 calcium flux assay protocol, cells are pre-loaded with Fura-2 AM (which can cross the cell membrane). Once inside the cell, the lipophilic blocking groups of Fura-2 AM are cleaved by esterases, resulting in a negatively charged fluorescent dye that stays inside the cell.
The excitation maxima of the dye shifts from 363 nm to 335 nm upon binding of the dye to calcium. The emission spectra is relatively unaffected. The change is most easily measured by comparing the emission at 510 nm on excitation separately at 380 nm and 340 nm.
Measuring the ratio reduces the effects of uneven dye loading and cell numbers, dye leakage and photo bleaching, compared to measuring cellular calcium concentrations using a single wavelength dye like Fluo-8.
Fura-2 calcium flux assay protocol summary:
- add Fura-2 solution to cells
- incubate for 1 hr at 37°C, then for 20 min at room temp
- analyze with a microplate reader at both Ex/Em 340/510 nm and Ex/Em 380/510nm
This product is intended to be used for monitoring calcium fluctuations in vivo in live cells using the following HTS imaging plate readers: FLIPR™, FDSS, BMG NOVOstar™, FLexStation, ViewLux, IN Cell Analyzer or Arrayscan.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Calcium ions (Ca²⁺) serve as essential signaling molecules in various cellular processes. These ions have an atomic mass number of 40.08. Calcium ions are highly concentrated in bone tissues teeth and within cells mostly stored in the endoplasmic reticulum and mitochondria. Common indicators of calcium concentration in research include the use of dyes such as Fura-2 and Calbryte 520 AM. These indicators help in visualizing the transient increases in intracellular calcium levels which are important in cellular signaling.
- Biological function summary
Calcium ions mediate various physiological functions including muscle contraction neurotransmitter release and hormone secretion. They typically do not act as lone agents; instead they often function as part of protein complexes particularly with calcium-binding proteins like calmodulin and troponin C. Calcium ions also interact with enzymes such as protein kinases and phosphatases regulating metabolic pathways and genetic expression.
- Pathways
Calcium ions play pivotal roles in the calcium signaling pathway and the cardiac excitation-contraction coupling pathway. In the calcium signaling pathway calcium ions once released from stores like the endoplasmic reticulum activate proteins such as calmodulin which then modulates various cellular activities. In the cardiac excitation-contraction coupling pathway calcium bows its link to proteins like sarcoplasmic reticulum calcium ATPase essential for heart muscle function.
- Associated diseases and disorders
Calcium ion dysregulation can lead to conditions such as osteoporosis and cardiac arrhythmias. In osteoporosis low calcium levels contribute to decreased bone density affecting bone health and making them prone to fractures. In cardiac arrhythmias abnormal calcium handling can result in irregular heartbeats. Proteins such as calbindin-D28k are closely connected to calcium metabolism disorders highlighting the significance of calcium in maintaining physiological balance.
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Functional Studies - Calcium Flux Assay Kit (Fura-2, No Wash, Ratiometric) (ab176766)
CHO-K1 cells were seeded overnight at 40,000 cells/100 μL/well in a Costar black wall/clear bottom 96-well plate. The cells were incubated with 100 μL of the Calcium Flux Assay Kit (Fura-2, No Wash, Ratiometric) (ab176766) for 1 hour at room temperature. ATP (50 μL/well) was added to achieve the final indicated concentrations.
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