Calpain Activity Assay Kit

• FuncS

Supplier Data

Functional Studies - Calpain Activity Assay Kit (AB65308)

Typical Data for ab65308 : Active Calpain (1 µg) was incubated at 37 °C for one hour using the Calpain Substrate with or without 20 µM Calpain Inhibitor.

• FuncS

Lab

Functional Studies - Calpain Activity Assay Kit (AB65308)

10e7 Jurkat cells (in 10 mL) were cultured in the absence or presence of 10 μM Camptothecin (CPT) (ab120115) or 10 μg/mL Cycloheximide (CHX) (ab120093) for 4 hours. Pelleted cells were lysed in 0.5 mL of Extraction Buffer and tested directly. Background signal subtracted, duplicates; +/- SD.

• FuncS

Lab

Functional Studies - Calpain Activity Assay Kit (AB65308)

Different amounts of positive control (Calpain I) treated with 1 μL of inhibitor (Z-LLY-FMK), background signal subtracted, duplicates; +/- SD.

• Other

Supplier Data

Other - Calpain Activity Assay Kit (AB65308)

Representative image of Calpain Activity Assay Kit ab65308

Components shown from left to right :

- Calpain I Positive Control

- 10X Reaction Buffer

- Calpain Substrate

- Calpain Inhibitor

- Extraction Buffer

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.