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AB284550

Carbonic Anhydrase (CA) Activity Assay Kit (Colorimetric)

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Carbonic Anhydrase (CA) Activity Assay Kit ab284550 is used to measure CA activity in biological samples like serum and hemolysates. Carbonic anhydrase acts on an ester substrate, releasing a chromophore that can be easily quantified using an absorbance microplate reader.

- Kit includes standard curve for quantitation, inhibitor control, and CA enzyme positive control
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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Functional Studies - Carbonic Anhydrase (CA) Activity Assay Kit (Colorimetric) (AB284550)
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Supplier Data

Functional Studies - Carbonic Anhydrase (CA) Activity Assay Kit (Colorimetric) (AB284550)

Carbonic Anhydrase Activity Assay Kit.

Kinetic activity curves using different amounts of CA Positive Control in the assay.

Functional Studies - Carbonic Anhydrase (CA) Activity Assay Kit (Colorimetric) (AB284550)
  • FuncS

Supplier Data

Functional Studies - Carbonic Anhydrase (CA) Activity Assay Kit (Colorimetric) (AB284550)

Carbonic Anhydrase Activity Assay Kit.

Specific CA activity in hemolysate and serum was measured after diluting 10X and then 2 μl of the diluted sample was used for the assay.

Functional Studies - Carbonic Anhydrase (CA) Activity Assay Kit (Colorimetric) (AB284550)
  • FuncS

Supplier Data

Functional Studies - Carbonic Anhydrase (CA) Activity Assay Kit (Colorimetric) (AB284550)

Carbonic Anhydrase Activity Assay Kit.

Nitrophenol-Standard Curve (8-40 nmol), error bars indicate SD (n=3)

Key facts

Detection method

Colorimetric

Sample types

Serum

Results type

Quantitative

Assay Platform

Microplate (12 x 8 well strips)

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Biochemicals

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Carbonic Anhydrase (CA) Activity Assay Kit (ab284550) can be used to measure CA activity in biological samples like serum and hemolysates, and also purified CA enzymes.

The assay utilizes the esterase activity of an active CA on an ester substrate which releases a chromophore. The released product can be easily quantified using an absorbance microplate reader. In the presence of a CA specific inhibitor, the enzyme loses its activity which results in a decrease of absorbance.

Other notes
This product was previously called K472 Carbonic Anhydrase (CA) Activity Assay Kit (Colorimetric). Biovision was acquired by Abcam in 2021.

Carbonic anhydrases (CA) are zinc enzymes present in both prokaryotes and eukaryotes. They efficiently catalyze the reversible hydration of CO2 to bicarbonate.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab284550-100Test", "assetComponentDetails": [ { "size":"1 x 1.5 mL", "name":"Dilution Buffer VI", "number":"AB284550-CMP02", "productcode":"" }, { "size":"1 x 400 µL", "name":"Nitrophenol Standard", "number":"AB284550-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"CA Inhibitor I", "number":"AB284550-CMP03", "productcode":"" }, { "size":"1 x 40 mL", "name":"CA Assay Buffer", "number":"AB284550-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"CA Enzyme I", "number":"AB284550-CMP04", "productcode":"" }, { "size":"1 x 500 µL", "name":"CA Substrate", "number":"AB284550-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

See full target information Carbonic Anhydrase
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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