Carbonic Anhydrase (CA) Activity Assay Kit ab284550 is used to measure CA activity in biological samples like serum and hemolysates. Carbonic anhydrase acts on an ester substrate, releasing a chromophore with colorimetric readout plate reader.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Images
Key facts
- Detection method
Colorimetric
- Sample types
Serum
- Assay type
Quantitative
What's included?
Recommended products
Carbonic Anhydrase (CA) Activity Assay Kit ab284550 is used to measure CA activity in biological samples like serum and hemolysates. Carbonic anhydrase acts on an ester substrate, releasing a chromophore with colorimetric readout plate reader.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
Colorimetric
- Sample types
Serum
- Assay type
Quantitative
- Assay Platform
Microplate (12 x 8 well strips)
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Carbonic anhydrases (CA) are zinc enzymes present in both prokaryotes and eukaryotes. They efficiently catalyze the reversible hydration of CO2 to bicarbonate. Carbonic Anhydrase (CA) Activity Assay Kit (ab284550 - K472) can be used to measure CA activity in biological samples like serum and hemolysates. The assay utilizes the esterase activity of an active CA on an ester substrate which releases a chromophore. The released product can be easily quantified using an absorbance microplate reader. In the presence of a CA specific inhibitor, the enzyme loses its activity which results in a decrease of absorbance.
This assay kit (ab284550 - K472) is simple and can be used to measure CA activity in biological samples like hemolysate, serum and purified CA activity, CA activity in biological samples like hemolysate and serum in a high-throughput format.
This product is manufactured by BioVision, an Abcam company and was previously called K472 Carbonic Anhydrase (CA) Activity Assay Kit (Colorimetric). K472-100 is the same size as the 100 test size of ab284550.
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3 product images
Functional Studies - Carbonic Anhydrase (CA) Activity Assay Kit (Colorimetric) (ab284550)
Carbonic Anhydrase Activity Assay Kit.
Nitrophenol-Standard Curve (8-40 nmol), error bars indicate SD (n=3)
Functional Studies - Carbonic Anhydrase (CA) Activity Assay Kit (Colorimetric) (ab284550)
Carbonic Anhydrase Activity Assay Kit.
Kinetic activity curves using different amounts of CA Positive Control in the assay.
Functional Studies - Carbonic Anhydrase (CA) Activity Assay Kit (Colorimetric) (ab284550)
Carbonic Anhydrase Activity Assay Kit.
Specific CA activity in hemolysate and serum was measured after diluting 10X and then 2 μl of the diluted sample was used for the assay.
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