Carbonic Anhydrase (CA) Activity Assay Kit ab284550 is used to measure CA activity in biological samples like serum and hemolysates. Carbonic anhydrase acts on an ester substrate, releasing a chromophore that can be easily quantified using an absorbance microplate reader.
- Kit includes standard curve for quantitation, inhibitor control, and CA enzyme positive control
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum
- Assay type
- Quantitative
What's included?
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Carbonic Anhydrase (CA) Activity Assay Kit ab284550 is used to measure CA activity in biological samples like serum and hemolysates. Carbonic anhydrase acts on an ester substrate, releasing a chromophore that can be easily quantified using an absorbance microplate reader.
- Kit includes standard curve for quantitation, inhibitor control, and CA enzyme positive control
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum
- Assay type
- Quantitative
- Assay Platform
- Microplate (12 x 8 well strips)
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Carbonic Anhydrase (CA) Activity Assay Kit (ab284550) can be used to measure CA activity in biological samples like serum and hemolysates, and also purified CA enzymes.
The assay utilizes the esterase activity of an active CA on an ester substrate which releases a chromophore. The released product can be easily quantified using an absorbance microplate reader. In the presence of a CA specific inhibitor, the enzyme loses its activity which results in a decrease of absorbance.
Other notes
This product was previously called K472 Carbonic Anhydrase (CA) Activity Assay Kit (Colorimetric). Biovision was acquired by Abcam in 2021.
Carbonic anhydrases (CA) are zinc enzymes present in both prokaryotes and eukaryotes. They efficiently catalyze the reversible hydration of CO2 to bicarbonate.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
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3 product images
Functional Studies - Carbonic Anhydrase (CA) Activity Assay Kit (Colorimetric) (ab284550)
Carbonic Anhydrase Activity Assay Kit.
Kinetic activity curves using different amounts of CA Positive Control in the assay.
Functional Studies - Carbonic Anhydrase (CA) Activity Assay Kit (Colorimetric) (ab284550)
Carbonic Anhydrase Activity Assay Kit.
Nitrophenol-Standard Curve (8-40 nmol), error bars indicate SD (n=3)
Functional Studies - Carbonic Anhydrase (CA) Activity Assay Kit (Colorimetric) (ab284550)
Carbonic Anhydrase Activity Assay Kit.
Specific CA activity in hemolysate and serum was measured after diluting 10X and then 2 μl of the diluted sample was used for the assay.
Downloads
Product protocols
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