Skip to main content

Carbonic Anhydrase (CA) Activity Assay Kit ab284550 is used to measure CA activity in biological samples like serum and hemolysates. Carbonic anhydrase acts on an ester substrate, releasing a chromophore with colorimetric readout plate reader.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Be the first to review this product! Submit a review

Images

Functional Studies - Carbonic Anhydrase (CA) Activity Assay Kit (Colorimetric) (AB284550), expandable thumbnail
  • Functional Studies - Carbonic Anhydrase (CA) Activity Assay Kit (Colorimetric) (AB284550), expandable thumbnail
  • Functional Studies - Carbonic Anhydrase (CA) Activity Assay Kit (Colorimetric) (AB284550), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Serum

Assay type

Quantitative

What's included?

100 Test
Components
CA Assay Buffer
1 x 40 mL
CA Enzyme I
1 x 1 Vial
CA Inhibitor I
1 x 200 µL
CA Substrate
1 x 500 µL
Dilution Buffer VI
1 x 1.5 mL
Nitrophenol Standard
1 x 400 µL

Recommended products

Carbonic Anhydrase (CA) Activity Assay Kit ab284550 is used to measure CA activity in biological samples like serum and hemolysates. Carbonic anhydrase acts on an ester substrate, releasing a chromophore with colorimetric readout plate reader.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Key facts

Detection method

Colorimetric

Sample types

Serum

Assay type

Quantitative

Assay Platform

Microplate (12 x 8 well strips)

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Carbonic anhydrases (CA) are zinc enzymes present in both prokaryotes and eukaryotes. They efficiently catalyze the reversible hydration of CO2 to bicarbonate. Carbonic Anhydrase (CA) Activity Assay Kit (ab284550 - K472) can be used to measure CA activity in biological samples like serum and hemolysates. The assay utilizes the esterase activity of an active CA on an ester substrate which releases a chromophore. The released product can be easily quantified using an absorbance microplate reader. In the presence of a CA specific inhibitor, the enzyme loses its activity which results in a decrease of absorbance.

This assay kit (ab284550 - K472) is simple and can be used to measure CA activity in biological samples like hemolysate, serum and purified CA activity, CA activity in biological samples like hemolysate and serum in a high-throughput format.

This product is manufactured by BioVision, an Abcam company and was previously called K472 Carbonic Anhydrase (CA) Activity Assay Kit (Colorimetric). K472-100 is the same size as the 100 test size of ab284550.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Functional Studies - Carbonic Anhydrase (CA) Activity Assay Kit (Colorimetric) (ab284550), expandable thumbnail

    Functional Studies - Carbonic Anhydrase (CA) Activity Assay Kit (Colorimetric) (ab284550)

    Carbonic Anhydrase Activity Assay Kit.

    Nitrophenol-Standard Curve (8-40 nmol), error bars indicate SD (n=3)

  • Functional Studies - Carbonic Anhydrase (CA) Activity Assay Kit (Colorimetric) (ab284550), expandable thumbnail

    Functional Studies - Carbonic Anhydrase (CA) Activity Assay Kit (Colorimetric) (ab284550)

    Carbonic Anhydrase Activity Assay Kit.

    Kinetic activity curves using different amounts of CA Positive Control in the assay.

  • Functional Studies - Carbonic Anhydrase (CA) Activity Assay Kit (Colorimetric) (ab284550), expandable thumbnail

    Functional Studies - Carbonic Anhydrase (CA) Activity Assay Kit (Colorimetric) (ab284550)

    Carbonic Anhydrase Activity Assay Kit.

    Specific CA activity in hemolysate and serum was measured after diluting 10X and then 2 μl of the diluted sample was used for the assay.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com