Caspase-1 Assay Kit (Fluorometric) (ab39412) provides a simple and convenient method for detecting the activity of caspase-1, which recognizes the sequence YVAD.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Cell death & disease 14:6562023Polyphenol-rich diet mediates interplay between macrophage-neutrophil and gut microbiota to alleviate intestinal inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDandan Han,Yujun Wu,Dongdong Lu,Jiaman Pang,Jie Hu,Xiangyu Zhang,Zhenyu Wang,Guolong Zhang,Junjun WangPubMed 37813835
- Cells 12:2022Enhanced Activity of NLRP3 Inflammasome in the Lung of Patients with Anti-Synthetase Syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEspiridión Ramos-Martinez,Angel E Vega-Sánchez,Gloria Pérez-Rubio,Mayra Mejia,Ivette Buendía-Roldán,Montserrat I González-Pérez,Heidegger N Mateos-Toledo,Warrison A Andrade,Ramcés Falfán-Valencia,Jorge Rojas-SerranoPubMed 36611853
- Journal of translational medicine 20:5832022Disruption of C/EBPβ-Clec7a axis exacerbates neuroinflammatory injury via NLRP3 inflammasome-mediated pyroptosis in experimental neuropathic pain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDan Wu,Yanqiong Zhang,Chunhui Zhao,Qiuyue Li,Junhong Zhang,Jiaxin Han,Zhijian Xu,Junfang Li,Yan Ma,Ping Wang,Haiyu XuPubMed 36503542
- International journal of molecular sciences 23:2022Synthetic Secoisolariciresinol Diglucoside (LGM2605) Prevents Asbestos-Induced Inflammation and Genotoxic Cell Damage in Human Mesothelial Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRalph A Pietrofesa,Shampa Chatterjee,Yuwaraj Kadariya,Joseph R Testa,Steven M Albelda,Melpo Christofidou-SolomidouPubMed 36077483
- Redox biology 56:1024322022Macrophage SAMSN1 protects against sepsis-induced acute lung injury in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWanli Jiang,Chengtai Ma,Jiawei Bai,Xianjin DuPubMed 35981417
- Journal of advanced research 39:305-3182022Galacto-oligosaccharides alleviate lung inflammation by inhibiting NLRP3 inflammasome activation in vivo and in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Cai,Myrthe S Gilbert,Walter J J Gerrits,Gert Folkerts,Saskia BraberPubMed 35777914
- Oxidative medicine and cellular longevity 2022:94937102022MicroRNA-1224-5p Aggravates Sepsis-Related Acute Lung Injury in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBing Liu,Feng Chen,Ni-Tao Cheng,Zheng Tang,Xian-Guo Wang,Ming XuPubMed 35799888
- Biomedicines 10:2022Role of Polyphenol-Derived Phenolic Acid in Mitigation of Inflammasome-Mediated Anxiety and Depression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuth Iban-Arias,Maria Sebastian-Valverde,Henry Wu,Weiting Lyu,Qingli Wu,Jim Simon,Giulio Maria PasinettiPubMed 35740286
- Cell death & disease 13:5022022Card9 protects sepsis by regulating Ripk2-mediated activation of NLRP3 inflammasome in macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhen Xu,Daoqian Li,Wei Qu,Yuxin Yin,Shuping Qiao,Yanan Zhu,Sunan Shen,Yayi Hou,Jie Yang,Tingting WangPubMed 35618701
- Disease markers 2022:45356522022RGS6 Drives Spinal Cord Injury by Inhibiting AMPK Pathway in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenxin Dao,Zhe Xiao,Weize Yang,Xiaomin Luo,Hongxia Xia,Zuneng LuPubMed 35510037
Key facts
- Detection method
Fluorescent
- Sample types
Tissue Extracts, Cell Lysate
- Assay type
Enzyme activity
- Assay time
2h
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Target data
Function
Thiol protease involved in a variety of inflammatory processes by proteolytically cleaving other proteins, such as the precursors of the inflammatory cytokines interleukin-1 beta (IL1B) and interleukin 18 (IL18) as well as the pyroptosis inducer Gasdermin-D (GSDMD), into active mature peptides (PubMed:15326478, PubMed:1574116, PubMed:7876192, PubMed:15498465, PubMed:26375003, PubMed:32051255). Plays a key role in cell immunity as an inflammatory response initiator: once activated through formation of an inflammasome complex, it initiates a proinflammatory response through the cleavage of the two inflammatory cytokines IL1B and IL18, releasing the mature cytokines which are involved in a variety of inflammatory processes (PubMed:1574116, PubMed:7876192, PubMed:15498465, PubMed:15326478, PubMed:32051255). Cleaves a tetrapeptide after an Asp residue at position P1 (PubMed:1574116, PubMed:7876192, PubMed:15498465). Also initiates pyroptosis, a programmed lytic cell death pathway, through cleavage of GSDMD (PubMed:26375003). In contrast to cleavage of interleukins IL1B and IL1B, recognition and cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part (PubMed:32051255, PubMed:32109412, PubMed:32553275). Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive (PubMed:28314590). In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly (PubMed:20197547).Isoform DeltaApoptosis inactive.Isoform EpsilonApoptosis inactive.
Alternative names
Caspase-1, CASP-1, Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme, p45, IL-1BC, ICE, IL-1 beta-converting enzyme, IL1BCE, IL1BC, CASP1
What's included?
Recommended products
Caspase-1 Assay Kit (Fluorometric) (ab39412) provides a simple and convenient method for detecting the activity of caspase-1, which recognizes the sequence YVAD.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Alternative names
Caspase-1, CASP-1, Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme, p45, IL-1BC, ICE, IL-1 beta-converting enzyme, IL1BCE, IL1BC, CASP1
Key facts
- Detection method
Fluorescent
- Sample types
Tissue Extracts, Cell Lysate
- Assay type
Enzyme activity
- Assay time
2h
- Assay Platform
Microplate reader
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Caspase-1 Assay Kit (Fluorometric) (ab39412) provides a simple and convenient method for detecting the activity of caspase-1, which recognizes the sequence YVAD.
The caspase-1 assay protocol is based on the cleavage of substrate YVAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). YVAD-AFC emits blue light (Em=400 nm); upon cleavage of the substrate by caspase-1 or related caspases, free AFC emits a yellow-green fluorescence (Ex/Em=400/505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence from a treated sample with an untreated control allows determination of the fold increase in caspase-1 activity.
Caspase-1 assay protocol summary:
- add samples to wells
- add reaction buffer and YVAD-AFC substrate and incubate for 1-2 hr
- analyze with a microplate reader
Caspase-1 (ICE, IL-1beta Converting Enzyme) is the prototypical member of the ICE family of proteases/caspases. **Other caspase and apoptosis assays** Review the full set of , or the .
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2 product images
Image from Badding M.A et al., PLoS One 10(4). Fig 6b. doi: 10.1371/journal.pone.0124368. Reproduced under the Creative Commons license https://creativecommons.org/publicdomain/zero/1.0/Functional Studies - Caspase-1 Assay Kit (Fluorometric) (ab39412)
Badding M.A et al investigated the cytotoxicity of Indium-tin oxide (ITO). Previously,ITO had shown to be cytotoxic in cultured cells and pro-inflammatory in pulmonary animal models. ITO is used to make transparent conductive coatings for touch screens and liquid crystal display electronics. RAW cells were plated at 5 x 105 cells/well and treated with SITO, LPS, or Min-U-sil (cells were first primed with LPS). Cells were washed, lysed, and 100 μg of lysates were assayed using Caspase 1 assay kit (ab39412). PBS was used as a control. All conditions were run in duplicate wells and three independent experiments were performed for each time point.
Functional Studies - Caspase-1 Assay Kit (Fluorometric) (ab39412)
Titration of the caspase 1 (Recombinant human Caspase-1 protein (Active) ab39901) (background signal subtracted, duplicates; +/- SD).
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