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AB219915

Caspase-3/7, Caspase-8 and Caspase-9 Multiplex Activity Assay Kit (Fluorometric)

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(40 Publications)

Caspase-3/7, Caspase-8 and Caspase-9 Multiplex Activity Assay Kit (Fluorometric) ab219915 is used to monitor multiple caspase activation in live cells

- 3 fluorogenic indicators for simultaneous monitoring of caspase3/7, caspase 8 and caspase 9
- Optimised for microplate reader
- Up to 100 tests for each caspase

View Alternative Names

CPP32, CASP3, Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CPP-32, SCA-1, MCH6, CASP9, Caspase-9, CASP-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6, APAF-3, ICE-LAP6, MCH3, CASP7, Caspase-7, CASP-7, Apoptotic protease Mch-3, CMH-1, ICE-like apoptotic protease 3, ICE-LAP3, MCH5, CASP8, Caspase-8, CASP-8, Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, FADD-homologous ICE/ced-3-like protease, FADD-like ICE, ICE-like apoptotic protease 5, MORT1-associated ced-3 homolog, FLICE, MACH

3 Images
Functional Studies - Caspase-3/7, Caspase-8 and Caspase-9 Multiplex Activity Assay Kit (Fluorometric) (AB219915)
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Supplier Data

Functional Studies - Caspase-3/7, Caspase-8 and Caspase-9 Multiplex Activity Assay Kit (Fluorometric) (AB219915)

Detection of Caspase activity in Jurkat cells Caspase 3/7.

Caspase 3/7, Caspase 8 and Caspase 9 Multiplex Activity Assay Kit (Fluorometric) (ab219915). Detection of Caspase activity in Jurkat cells graphs shown Caspase 3/7 activity. Jurkat cells were seeded on the same day at 2 x 105 cells/well in a Costar black wall/clear bottom 96-well plate. Cells were either left untreated (black bar) or treated with 1 μM staurosporine for 4 hours (gray bar). Single-caspase assay loading solution (100 μL/well; #1 for Caspase 3/7, #2 for caspase 8 or #3 for caspase 9) or Triple-caspase assay loading solution (100 μL/well; #4 for Caspase 3/7, 8 and 9 together) was added to cells, followed by an incubation at RT for 1 hour. The fluorescence intensity was measured with FlexStation fluorescence microplate reader at the indicated wavelength. Caspase 3/7, 8 and 9 activities can be detected in a single assay without interferences from other caspases.

Functional Studies - Caspase-3/7, Caspase-8 and Caspase-9 Multiplex Activity Assay Kit (Fluorometric) (AB219915)
  • FuncS

Supplier Data

Functional Studies - Caspase-3/7, Caspase-8 and Caspase-9 Multiplex Activity Assay Kit (Fluorometric) (AB219915)

Detection of Caspase activity in Jurkat cells Caspase 9.

Caspase 3/7, Caspase 8 and Caspase 9 Multiplex Activity Assay Kit (Fluorometric) (ab219915). Detection of Caspase activity in Jurkat cells graphs shown Caspase 9 activity. Jurkat cells were seeded on the same day at 2 x 105 cells/well in a Costar black wall/clear bottom 96-well plate. Cells were either left untreated (black bar) or treated with 1 μM staurosporine for 4 hours (gray bar). Single-caspase assay loading solution (100 μL/well; #1 for Caspase 3/7, #2 for caspase 8 or #3 for caspase 9) or Triple-caspase assay loading solution (100 μL/well; #4 for Caspase 3/7, 8 and 9 together) was added to cells, followed by an incubation at RT for 1 hour. The fluorescence intensity was measured with FlexStation fluorescence microplate reader at the indicated wavelength. Caspase 3/7, 8 and 9 activities can be detected in a single assay without interferences from other caspases.

Functional Studies - Caspase-3/7, Caspase-8 and Caspase-9 Multiplex Activity Assay Kit (Fluorometric) (AB219915)
  • FuncS

Supplier Data

Functional Studies - Caspase-3/7, Caspase-8 and Caspase-9 Multiplex Activity Assay Kit (Fluorometric) (AB219915)

Detection of Caspase activity in Jurkat cells Caspase 8.

Caspase 3/7, Caspase 8 and Caspase 9 Multiplex Activity Assay Kit (Fluorometric) (ab219915). Detection of Caspase activity in Jurkat cells graphs shown Caspase 8 activity. Jurkat cells were seeded on the same day at 2 x 105 cells/well in a Costar black wall/clear bottom 96-well plate. Cells were either left untreated (black bar) or treated with 1 μM staurosporine for 4 hours (gray bar). Single-caspase assay loading solution (100 μL/well; #1 for Caspase 3/7, #2 for caspase 8 or #3 for caspase 9) or Triple-caspase assay loading solution (100 μL/well; #4 for Caspase 3/7, 8 and 9 together) was added to cells, followed by an incubation at RT for 1 hour. The fluorescence intensity was measured with FlexStation fluorescence microplate reader at the indicated wavelength. Caspase 3/7, 8 and 9 activities can be detected in a single assay without interferences from other caspases.

Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Results type

Semi-Quantitative

Assay Platform

Microplate reader

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Reactivity data

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Product details

Caspase-3/7, Caspase-8 and Caspase-9 Multiplex Activity Assay Kit (Fluorometric) is designed to simultaneously monitor three key caspases involved in apoptosis: the initiator caspases caspase 8 and caspase 9, and the executioner Caspase 3.

How the assay works

The kit uses DEVD-ProRed™, IETD-R110 and LEHD-AMC as fluorogenic indicators for Caspase 3, caspase 8 and caspase 9 activity respectively. Upon caspase cleavage, three distinct fluorophores are released: ProRed™ (red fluorescence), R110 (green fluorescence) and AMC (blue fluorescence), which can be readily monitored in a single assay due to their spectral separation.

This product has been optimized for use in a microplate reader in a 96-well plate, providing enough reagent to perform 100 tests for each caspase.

Caspase-3/7, Caspase-8 and Caspase-9 protocol summary

- Grow and treat cells with test compounds to induce caspase activation

- Add caspase assay solution to cells

- Incubate at room temperature for 30-60 minutes

- Monitor fluorescence intensity at: Ex/Em = 535/620 nm (Caspase 3/7), Ex/Em = 490/525 nm (Caspase 8), Ex/Em = 370/450 nm (Caspase 9)

How other researchers are using this kit

ab219915 has been used in a variety of cancer cell lines including MCF-7 (breast cancer) cell line (1), U87MG (human glioblastoma) and MES-SA (human uterine sarcoma) cell lines (2).

References: 1 - T. Kumkoon et al. 2023; 2 - Yu Hsia et al. 2023.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab219915-100Test", "assetComponentDetails": [ { "size":"1 x 50 µL", "name":"200X Caspase 9 Substrate", "number":"AB219915-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Buffer", "number":"AB219915-CMP01", "productcode":"" }, { "size":"1 x 50 µL", "name":"200X Caspase 3/7 Substrate", "number":"AB219915-CMP02", "productcode":"" }, { "size":"1 x 50 µL", "name":"200X Caspase 8 Substrate", "number":"AB219915-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis (PubMed : 18723680, PubMed : 20566630, PubMed : 23650375, PubMed : 35338844, PubMed : 35446120, PubMed : 7596430). Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins (PubMed : 18723680, PubMed : 20566630, PubMed : 23650375, PubMed : 7596430). At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a '216-Asp-|-Gly-217' bond (PubMed : 10497198, PubMed : 16374543, PubMed : 7596430, PubMed : 7774019). Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain (By similarity). Cleaves and activates caspase-6, -7 and -9 (CASP6, CASP7 and CASP9, respectively) (PubMed : 7596430). Cleaves and inactivates interleukin-18 (IL18) (PubMed : 37993714, PubMed : 9334240). Involved in the cleavage of huntingtin (PubMed : 8696339). Triggers cell adhesion in sympathetic neurons through RET cleavage (PubMed : 21357690). Cleaves DSG2 in response to apoptosis resulting in a loss of full length DSG2 at desmosome cell junctions and subsequent loss of cell-cell adhesion (PubMed : 17559062). Also cleaves JUP in response to apoptosis (PubMed : 17559062). Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress (PubMed : 23152800). Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction (PubMed : 30878284). Also involved in pyroptosis by mediating cleavage and activation of gasdermin-E (GSDME) (PubMed : 35338844, PubMed : 35446120). Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface (PubMed : 23845944, PubMed : 33725486). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed : 36758104, PubMed : 36758106).
See full target information CASP3

Additional targets

CASP8,CASP9,CASP7
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Publications (40)

Recent publications for all applications. Explore the full list and refine your search

Pharmacological reports : PR 77:1347-1363 PubMed40553247

2025

A novel 2-oxopyrrolidine derivative (LN-53) efficiently induces Nrf-2 signaling pathway activation in human epidermal keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Basak Ezgi Sarac,Laura Nissim,Dilara Karaguzel,Gokhan Arik,Shirin Kahremany,Edward E Korshin,Arie Gruzman,Cagatay Karaaslan

International journal of molecular sciences 26: PubMed40244142

2025

MnO Nanocrystal-Induced Eryptosis Features Ca Overload, ROS and RNS Accumulation, Calpain Activation, Recruitment of Caspases, and Changes in the Lipid Order of Cell Membranes.

Applications

Unspecified application

Species

Unspecified reactive species

Yuriy Kot,Volodymyr Prokopiuk,Vladimir Klochkov,Liliya Tryfonyuk,Pavel Maksimchuk,Andrey Aslanov,Kateryna Kot,Oleg Avrunin,Lesya Demchenko,Saulesh Kurmangaliyeva,Anatolii Onishchenko,Svetlana Yefimova,Ondrej Havranek,Anton Tkachenko

International journal of molecular sciences 26: PubMed39940782

2025

Combination of Low-Dose Sulforaphane and Docetaxel on Mitochondrial Function and Metabolic Reprogramming in Prostate Cancer Cell Lines.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Peñata-Taborda,Pedro Espitia-Pérez,Lyda Espitia-Pérez,Andrés Coneo-Pretelt,Hugo Brango,Dina Ricardo-Caldera,Gean Arteaga-Arroyo,Luisa Jiménez-Vidal,Claudia Galeano-Páez,Karina Pastor-Sierra,Alicia Humanez-Alvarez,Osnamir Bru-Cordero,Nathalia Jones-Cifuentes,Bladimiro Rincón-Orozco,Stelia Mendez-Sanchez,Mario Negrette-Guzmán

International journal of molecular sciences 25: PubMed39519237

2024

GdVO:Eu and LaVO:Eu Nanoparticles Exacerbate Oxidative Stress in L929 Cells: Potential Implications for Cancer Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Yuriy Kot,Vladimir Klochkov,Volodymyr Prokopiuk,Olha Sedyh,Liliya Tryfonyuk,Ganna Grygorova,Nina Karpenko,Oleksandr Tomchuk,Kateryna Kot,Anatolii Onishchenko,Svetlana Yefimova,Anton Tkachenko

Nature communications 15:8002 PubMed39266533

2024

SHANK3 depletion leads to ERK signalling overdose and cell death in KRAS-mutant cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Johanna Lilja,Jasmin Kaivola,James R W Conway,Joni Vuorio,Hanna Parkkola,Pekka Roivas,Michal Dibus,Megan R Chastney,Taru Varila,Guillaume Jacquemet,Emilia Peuhu,Emily Wang,Ulla Pentikäinen,Itziar Martinez D Posada,Hellyeh Hamidi,Arafath K Najumudeen,Owen J Sansom,Igor L Barsukov,Daniel Abankwa,Ilpo Vattulainen,Marko Salmi,Johanna Ivaska

Scientific reports 14:14674 PubMed38918539

2024

New hemisynthetic derivatives of sphaeropsidin phytotoxins triggering severe endoplasmic reticulum swelling in cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Aude Ingels,Robert Scott,Annie R Hooper,Aletta E van der Westhuyzen,Sachin B Wagh,Joséphine de Meester,Lucia Maddau,Doris Marko,Georg Aichinger,Walter Berger,Marjorie Vermeersch,David Pérez-Morga,Vladimir A Maslivetc,Antonio Evidente,Willem A L van Otterlo,Alexander Kornienko,Véronique Mathieu

RSC advances 14:19490-19504 PubMed38895533

2024

The phototoxic effect of a gold-antibody-based nanocarrier of phthalocyanine on melanoma monolayers and tumour spheroids.

Applications

Unspecified application

Species

Unspecified reactive species

Nkune Williams Nkune,Heidi Abrahamse

Journal of translational medicine 22:221 PubMed38429788

2024

Thyroid hormone protects human lung epithelial cells from cold preservation and warm reperfusion-induced injury.

Applications

Unspecified application

Species

Unspecified reactive species

Dejan Bojic,Tanroop Aujla,Junichi Sugihara,Aaron Wong,Shaf Keshavjee,Mingyao Liu

ChemMedChem 19:e202300680 PubMed38323458

2024

Effective Anticancer Potential of a New Sulfonamide as a Carbonic Anhydrase IX Inhibitor Against Aggressive Tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Ismail Koyuncu,Ebru Temiz,Eray Metin Güler,Mustafa Durgun,Ozgür Yuksekdag,Simone Giovannuzzi,Claudiu T Supuran

Cell death & disease 15:67 PubMed38238320

2024

Non-alcoholic fatty liver disease promotes breast cancer progression through upregulated hepatic fibroblast growth factor 21.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Sui,Qingqing Liu,Cong Xu,Kumar Ganesan,Zhen Ye,Yan Li,Jianmin Wu,Bing Du,Fei Gao,Cailu Song,Jianping Chen
View all publications
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