Caspase-3 Assay Kit (Colorimetric) ab39401 provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Colorimetric
Tissue Lysate, Cell Lysate
Enzyme activity
2h
Select an associated product type
Involved in the activation cascade of caspases responsible for apoptosis execution (PubMed:7596430). At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond (PubMed:7774019). Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9 (PubMed:7596430). Involved in the cleavage of huntingtin (PubMed:8696339). Triggers cell adhesion in sympathetic neurons through RET cleavage (PubMed:21357690). Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress (PubMed:23152800).
Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CPP-32, SCA-1, CASP3, CPP32
Caspase-3 Assay Kit (Colorimetric) ab39401 provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CPP-32, SCA-1, CASP3, CPP32
Colorimetric
Tissue Lysate, Cell Lysate
Enzyme activity
2h
Microplate reader
Blue Ice
-20°C
-20°C
-20°C
Caspase-3 Assay Kit (Colorimetric) ab39401 provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD.
The Caspase-3 assay protocol is based on the formation of the chromophore p-nitroaniline (p-NA) by cleavage from the labeled substrate DEVD-pNA. The p-NA can be quantified using a spectrophotometer or a microtiter plate reader reading absorbance at 400 or 405 nm.
Comparison of the absorbance of p-NA from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-3 activity.
Caspase-3 assay protocol summary:
- add samples to wells
- add reaction buffer and DEVD-p-NA substrate and incubate for 60-120 min at 37°C
- analyze with microplate reader
Due to the nature of the substrate, this assay also detects caspase-7 activity. Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. **Other caspase and apoptosis assays** Review the full set of , or the .
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H9c2 cells were either untreated (control) or treated with 2μg/ml of recombinant lipocalin-2 (Lcn2) for 24h. Cell lysates were assayed for caspase-3 activity (n = 3, *, p<0.05).
Image obtained from Xu G et al., JBC, 2012 Feb 11;28(7):4808-17
Caspase-3 in Jurkat lysates (3.3 x10e6 cells) following 20 hour exposure to 2 uM Camptothecin (Camptothecin, DNA topoisomerase inhibitor ab120115) or 10 ng/mL anti-Fas Ab (MBL).
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