Skip to main content

Caspase-3 Assay Kit (Colorimetric) ab39401 provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.


Images

Functional Studies - Caspase-3 Assay Kit (Colorimetric) (AB39401), expandable thumbnail
  • Functional Studies - Caspase-3 Assay Kit (Colorimetric) (AB39401), expandable thumbnail

Publications

Key facts

Detection method

Colorimetric

Sample types

Tissue Lysate, Cell Lysate

Assay type

Enzyme activity

Assay time

2h

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Involved in the activation cascade of caspases responsible for apoptosis execution (PubMed:7596430). At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond (PubMed:7774019). Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9 (PubMed:7596430). Involved in the cleavage of huntingtin (PubMed:8696339). Triggers cell adhesion in sympathetic neurons through RET cleavage (PubMed:21357690). Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress (PubMed:23152800).

Alternative names

What's included?

100 Test
Components
2X Reaction Buffer I
4 x 2 mL
DEVD-pNA
1 x 500 µL
DTT I
1 x 400 µL
Dilution Buffer II
1 x 100 mL
Lysis Buffer IV
1 x 100 mL

Recommended products

Caspase-3 Assay Kit (Colorimetric) ab39401 provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Tissue Lysate, Cell Lysate

Assay type

Enzyme activity

Assay time

2h

Assay Platform

Microplate reader

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Caspase-3 Assay Kit (Colorimetric) ab39401 provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD.

The Caspase-3 assay protocol is based on the formation of the chromophore p-nitroaniline (p-NA) by cleavage from the labeled substrate DEVD-pNA. The p-NA can be quantified using a spectrophotometer or a microtiter plate reader reading absorbance at 400 or 405 nm.

Comparison of the absorbance of p-NA from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-3 activity.

Caspase-3 assay protocol summary:
- add samples to wells
- add reaction buffer and DEVD-p-NA substrate and incubate for 60-120 min at 37°C
- analyze with microplate reader

Due to the nature of the substrate, this assay also detects caspase-7 activity. Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. **Other caspase and apoptosis assays** Review the full set of , or the .

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Functional Studies - Caspase-3 Assay Kit (Colorimetric) (ab39401), expandable thumbnail
    Image from Xu G. et al. J Biol Chem. 2012;287(7):4808–4817. doi:10.1074/jbc.M111.275719. Fig. 2G.

    Functional Studies - Caspase-3 Assay Kit (Colorimetric) (ab39401)

    H9c2 cells were either untreated (control) or treated with 2μg/ml of recombinant lipocalin-2 (Lcn2) for 24h. Cell lysates were assayed for caspase-3 activity (n = 3, *, p<0.05).
    Image obtained from Xu G et al., JBC, 2012 Feb 11;28(7):4808-17

  • Functional Studies - Caspase-3 Assay Kit (Colorimetric) (ab39401), expandable thumbnail

    Functional Studies - Caspase-3 Assay Kit (Colorimetric) (ab39401)

    Caspase-3 in Jurkat lysates (3.3 x10e6 cells) following 20 hour exposure to 2 uM Camptothecin (Camptothecin, DNA topoisomerase inhibitor ab120115) or 10 ng/mL anti-Fas Ab (MBL).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com