Caspase-3 Assay Kit (Colorimetric) ab39401 provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Biomolecules & therapeutics 32:349-3602024Morroniside Protects C2C12 Myoblasts from Oxidative Damage Caused by ROS-Mediated Mitochondrial Damage and Induction of Endoplasmic Reticulum Stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyun Hwangbo,Cheol Park,EunJin Bang,Hyuk Soon Kim,Sung-Jin Bae,Eunjeong Kim,Youngmi Jung,Sun-Hee Leem,Young Rok Seo,Su Hyun Hong,Gi-Young Kim,Jin Won Hyun,Yung Hyun ChoiPubMed 38602043
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Key facts
- Detection method
Colorimetric
- Sample types
Tissue Lysate, Cell Lysate
- Assay type
Enzyme activity
- Assay time
2h
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Target data
Function
Involved in the activation cascade of caspases responsible for apoptosis execution (PubMed:7596430). At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond (PubMed:7774019). Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9 (PubMed:7596430). Involved in the cleavage of huntingtin (PubMed:8696339). Triggers cell adhesion in sympathetic neurons through RET cleavage (PubMed:21357690). Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress (PubMed:23152800).
Alternative names
Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CPP-32, SCA-1, CASP3, CPP32
What's included?
Recommended products
Caspase-3 Assay Kit (Colorimetric) ab39401 provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Alternative names
Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CPP-32, SCA-1, CASP3, CPP32
Key facts
- Detection method
Colorimetric
- Sample types
Tissue Lysate, Cell Lysate
- Assay type
Enzyme activity
- Assay time
2h
- Assay Platform
Microplate reader
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Caspase-3 Assay Kit (Colorimetric) ab39401 provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD.
The Caspase-3 assay protocol is based on the formation of the chromophore p-nitroaniline (p-NA) by cleavage from the labeled substrate DEVD-pNA. The p-NA can be quantified using a spectrophotometer or a microtiter plate reader reading absorbance at 400 or 405 nm.
Comparison of the absorbance of p-NA from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-3 activity.
Caspase-3 assay protocol summary:
- add samples to wells
- add reaction buffer and DEVD-p-NA substrate and incubate for 60-120 min at 37°C
- analyze with microplate reader
Due to the nature of the substrate, this assay also detects caspase-7 activity. Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. **Other caspase and apoptosis assays** Review the full set of , or the .
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2 product images
Image from Xu G. et al. J Biol Chem. 2012;287(7):4808–4817. doi:10.1074/jbc.M111.275719. Fig. 2G.Functional Studies - Caspase-3 Assay Kit (Colorimetric) (ab39401)
H9c2 cells were either untreated (control) or treated with 2μg/ml of recombinant lipocalin-2 (Lcn2) for 24h. Cell lysates were assayed for caspase-3 activity (n = 3, *, p<0.05).
Image obtained from Xu G et al., JBC, 2012 Feb 11;28(7):4808-17
Functional Studies - Caspase-3 Assay Kit (Colorimetric) (ab39401)
Caspase-3 in Jurkat lysates (3.3 x10e6 cells) following 20 hour exposure to 2 uM Camptothecin (Camptothecin, DNA topoisomerase inhibitor ab120115) or 10 ng/mL anti-Fas Ab (MBL).
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