Caspase-3 Assay Kit (Colorimetric) is a rapid and easy to use kit to assay the activity of caspases that recognisze the DEVD sequence.
- Colorimetric assay - 400 nm readout - works on standard microplate readers
- Determine the fold-increase in Caspase-3 activity in just over 2 hours
- Cited in over 320 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Biomolecules & therapeutics 32:349-3602024Morroniside Protects C2C12 Myoblasts from Oxidative Damage Caused by ROS-Mediated Mitochondrial Damage and Induction of Endoplasmic Reticulum Stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyun Hwangbo,Cheol Park,EunJin Bang,Hyuk Soon Kim,Sung-Jin Bae,Eunjeong Kim,Youngmi Jung,Sun-Hee Leem,Young Rok Seo,Su Hyun Hong,Gi-Young Kim,Jin Won Hyun,Yung Hyun ChoiPubMed 38602043
- Archiv der Pharmazie 357:e23004352024Multipathway regulation induced by 4-(phenylsulfonyl)morpholine derivatives against triple-negative breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFan-Wei Yang,Te-Lun Mai,Ying-Chung Jimmy Lin,Yu-Chen Chen,Shang-Che Kuo,Chia-Ming Lin,Meng-Hsuan Lee,Jung-Chen SuPubMed 38314850
- Experimental and therapeutic medicine 27:552024PDCD4 silencing alleviates KA‑induced neurotoxicity of HT22 cells by inhibiting endoplasmic reticulum stress via blocking the MAPK/NF‑κB signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeng Li,Guiling CaoPubMed 38234627
- Science advances 9:eadi84542023Partial in vivo reprogramming enables injury-free intestinal regeneration via autonomous induction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJumee Kim,Somi Kim,Seung-Yeon Lee,Beom-Ki Jo,Ji-Young Oh,Eun-Ji Kwon,Keun-Tae Kim,Anish Ashok Adpaikar,Eun-Jung Kim,Han-Sung Jung,Hwa-Ryeon Kim,Jae-Seok Roe,Chang Pyo Hong,Jong Kyoung Kim,Bon-Kyoung Koo,Hyuk-Jin ChaPubMed 38000027
- Cardiovascular diabetology 22:3122023Treatment with recombinant Sirt1 rewires the cardiac lipidome and rescues diabetes-related metabolic cardiomyopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah Costantino,Alessandro Mengozzi,Srividya Velagapudi,Shafeeq Ahmed Mohammed,Era Gorica,Alexander Akhmedov,Alessia Mongelli,Nicola Riccardo Pugliese,Stefano Masi,Agostino Virdis,Andreas Hülsmeier,Christian Matthias Matter,Thorsten Hornemann,Giovanni Melina,Frank Ruschitzka,Thomas Felix Luscher,Francesco PaneniPubMed 37957697
- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e23023682023Targeting CBX3 with a Dual BET/PLK1 Inhibitor Enhances the Antitumor Efficacy of CDK4/6 Inhibitors in Prostate Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuaiyuan Liang,Chunguang Yang,Ruijiang Zeng,Yingqiu Song,Jianxi Wang,Wei Xiong,Binyuan Yan,Xin JinPubMed 37949681
- Genes 14:2023Effect of Glucose Supplementation on Apoptosis in the of Chickens Raised under Thermoneutral or Heat Stress Environment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJosephine Kwakye,Oluwatomide W Ariyo,Ahmed F A Ghareeb,Evan Hartono,Selorm Sovi,Bikash Aryal,Marie C Milfort,Alberta L Fuller,Romdhane Rekaya,Samuel E AggreyPubMed 37895271
- Cells 12:2023Propofol Inhibits Glioma Stem Cell Growth and Migration and Their Interaction with Microglia via BDNF-AS and Extracellular Vesicles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRephael Nizar,Simona Cazacu,Cunli Xiang,Matan Krasner,Efrat Barbiro-Michaely,Doron Gerber,Jonathan Schwartz,Iris Fried,Shira Yuval,Aharon Brodie,Gila Kazimirsky,Naama Amos,Ron Unger,Stephen Brown,Lisa Rogers,Donald H Penning,Chaya BrodiePubMed 37566001
- ACS omega 8:21948-219682023Synthesis, Characterization, DNA/HSA Interaction, and Cytotoxic Activity of a Copper(II) Thiolate Schiff Base Complex and Its Corresponding Water-Soluble Stable Sulfinato-O Complex Containing Imidazole as a Co-ligand.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKumudini Paliwal,Paramita Haldar,P K Sudhadevi Antharjanam,Manjuri KumarPubMed 37360467
- International journal of molecular medicine 52:2023Ginsenoside Rg1 regulates autophagy and endoplasmic reticulum stress via the AMPK/mTOR and PERK/ATF4/CHOP pathways to alleviate alcohol‑induced myocardial injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGe Tian,Jing Li,Lina ZhouPubMed 37232350
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Lysate, Cell Lysate
- Assay type
- Enzyme activity
- Assay time
- 2h
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Target data
Function
Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis (PubMed:18723680, PubMed:20566630, PubMed:23650375, PubMed:35338844, PubMed:35446120, PubMed:7596430). Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins (PubMed:18723680, PubMed:20566630, PubMed:23650375, PubMed:7596430). At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a '216-Asp-|-Gly-217' bond (PubMed:10497198, PubMed:16374543, PubMed:7596430, PubMed:7774019). Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain (By similarity). Cleaves and activates caspase-6, -7 and -9 (CASP6, CASP7 and CASP9, respectively) (PubMed:7596430). Cleaves and inactivates interleukin-18 (IL18) (PubMed:37993714, PubMed:9334240). Involved in the cleavage of huntingtin (PubMed:8696339). Triggers cell adhesion in sympathetic neurons through RET cleavage (PubMed:21357690). Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress (PubMed:23152800). Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction (PubMed:30878284). Also involved in pyroptosis by mediating cleavage and activation of gasdermin-E (GSDME) (PubMed:35338844, PubMed:35446120). Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface (PubMed:23845944, PubMed:33725486). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed:36758104, PubMed:36758106).
Alternative names
CPP32, CASP3, Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CPP-32, SCA-1
What's included?
Recommended products
Caspase-3 Assay Kit (Colorimetric) is a rapid and easy to use kit to assay the activity of caspases that recognisze the DEVD sequence.
- Colorimetric assay - 400 nm readout - works on standard microplate readers
- Determine the fold-increase in Caspase-3 activity in just over 2 hours
- Cited in over 320 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Lysate, Cell Lysate
- Assay type
- Enzyme activity
- Assay time
- 2h
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Caspase-3 Assay Kit (Colorimetric) ab39401 allows the rapid, sensitive and accurate measurement of Caspase-3 activity in cell lysates.
How the assay works
The Caspase-3 assay protocol is based on the formation of the chromophore p-nitroaniline (p-NA) by cleavage from the labeled substrate DEVD-pNA. The p-NA can be quantified using a spectrophotometer or a microtiter plate reader reading absorbance at 400 or 405 nm.
Comparison of the absorbance of p-NA from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-3 activity.
Caspase-3 Assay protocol summary
- Add samples to wells
- Add reaction buffer and DEVD-p-NA substrate and incubate for 60-120 min at 37°C
- Analyze with microplate reader
Note: Due to the nature of the substrate, this assay also detects caspase-7 activity.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Functional Studies - Caspase-3 Assay Kit (Colorimetric) (ab39401)
Caspase-3 in Jurkat lysates (3.3 x10e6 cells) following 20 hour exposure to 2 uM Camptothecin (Camptothecin, DNA topoisomerase inhibitor ab120115) or 10 ng/mL anti-Fas Ab (MBL).
Image from Xu G. et al. J Biol Chem. 2012;287(7):4808–4817. doi:10.1074/jbc.M111.275719. Fig. 2G.Functional Studies - Caspase-3 Assay Kit (Colorimetric) (ab39401)
H9c2 cells were either untreated (control) or treated with 2μg/ml of recombinant lipocalin-2 (Lcn2) for 24h. Cell lysates were assayed for caspase-3 activity (n = 3, *, p<0.05).
Image obtained from Xu G et al., JBC, 2012 Feb 11;28(7):4808-17
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