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Caspase-3 Assay Kit (Colorimetric) ab39401 provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD.
Kit components available for purchase. Minimum order: 20 units. Contact us to discuss your needs.
Is this your first time purchasing this product? Get 20% off one unit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, and Australia.
Alternative names=Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CPP-32, SCA-1, CASP3, CPP32
- Detection method
Colorimetric
- Sample types
Tissue Lysate, Cell Lysate
- Assay type
Enzyme activity
- Assay time
2h
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Caspase-3 Assay Kit (Colorimetric) ab39401 provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD.
Kit components available for purchase. Minimum order: 20 units. Contact us to discuss your needs.
Is this your first time purchasing this product? Get 20% off one unit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, and Australia.
Alternative names=Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CPP-32, SCA-1, CASP3, CPP32
Key facts
- Detection method
Colorimetric
- Sample types
Tissue Lysate, Cell Lysate
- Assay type
Enzyme activity
- Assay time
2h
- Assay Platform
Microplate reader
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Caspase-3 Assay Kit (Colorimetric) ab39401 provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD.
The Caspase-3 assay protocol is based on the formation of the chromophore p-nitroaniline (p-NA) by cleavage from the labeled substrate DEVD-pNA. The p-NA can be quantified using a spectrophotometer or a microtiter plate reader reading absorbance at 400 or 405 nm.
Comparison of the absorbance of p-NA from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-3 activity.
Caspase-3 assay protocol summary:
- add samples to wells
- add reaction buffer and DEVD-p-NA substrate and incubate for 60-120 min at 37°C
- analyze with microplate reader
Due to the nature of the substrate, this assay also detects caspase-7 activity. Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. **Other caspase and apoptosis assays** Review the full set of , or the .
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