Abcam's Caspase-3 Inhibitor Drug Detection Kit provides an effective means for screening caspase inhibitors using fluorometric methods.
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- Scientific reports 8:116532018A new quinoline-based chemical probe inhibits the autophagy-related cysteine protease ATG4B.Applications:Unspecified applicationReactive species:Unspecified reactive speciesD Bosc et. al.PubMed 30076329
Key facts
- Detection method
- Fluorescent
- Sample types
- Inhibitor compounds
- Assay type
- Quantitative
- Assay time
- 1h 30m
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Target data
Function
Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis (PubMed:18723680, PubMed:20566630, PubMed:23650375, PubMed:35338844, PubMed:35446120, PubMed:7596430). Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins (PubMed:18723680, PubMed:20566630, PubMed:23650375, PubMed:7596430). At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a '216-Asp-|-Gly-217' bond (PubMed:10497198, PubMed:16374543, PubMed:7596430, PubMed:7774019). Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain (By similarity). Cleaves and activates caspase-6, -7 and -9 (CASP6, CASP7 and CASP9, respectively) (PubMed:7596430). Cleaves and inactivates interleukin-18 (IL18) (PubMed:37993714, PubMed:9334240). Involved in the cleavage of huntingtin (PubMed:8696339). Triggers cell adhesion in sympathetic neurons through RET cleavage (PubMed:21357690). Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress (PubMed:23152800). Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction (PubMed:30878284). Also involved in pyroptosis by mediating cleavage and activation of gasdermin-E (GSDME) (PubMed:35338844, PubMed:35446120). Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface (PubMed:23845944, PubMed:33725486). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed:36758104, PubMed:36758106).
Alternative names
CPP32, CASP3, Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CPP-32, SCA-1
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Abcam's Caspase-3 Inhibitor Drug Detection Kit provides an effective means for screening caspase inhibitors using fluorometric methods.
Key facts
- Detection method
- Fluorescent
- Sample types
- Inhibitor compounds
- Assay type
- Quantitative
- Assay time
- 1h 30m
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's Caspase-3 Inhibitor Drug Detection Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate DEVD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active Caspase-3 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of Caspase-3 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
Visit our FAQs page for tips and troubleshooting.
This product is manufactured by BioVision, an Abcam company and was previously called K153 Caspase-3 Inhibitor Drug Screening Kit (Fluorometric). K153-100 is the same size as the 100 test size of ab102491.
Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
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3 product images
Functional Studies - Caspase-3 Inhibitor Assay Kit (ab102491)
Titration of the Caspase inhibitor Z-VAD(OMe)-FMK (Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor ab120487) (duplicates; +/- SD).
Functional Studies - Caspase-3 Inhibitor Assay Kit (ab102491)
Titration of the Caspase-3 specific inhibitor Q-DEVD-OPh (Q-DEVD-OPh, caspase-3 inhibitor ab142037) (duplicates; +/- SD).
Functional Studies - Caspase-3 Inhibitor Assay Kit (ab102491)
Caspase activity (RFU) in presence of 0μM - 40μM of z-VAD-FMK (generic caspase inhibitor), assessed using DEVD-AFC as caspase-3 substrate and following Caspase-3 Inhibitor Drug Detection Kit (ab102491) protocol. Lanes:
1.- Background Control: no Caspase-3, no z-VMAD-FMK
2.- Positive Inhibition Control: +Caspase-3, +z-VAD-FMK
3.- Positive Control: +Caspase-3, no z-VAD-FMK
4.- Caspase-3 + 2.5μM z-VAD-FMK
5.- Caspase-3 + 10μM z-VAD-FMK
6.- Caspase-3 + 40μM z-VAD-FMK
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