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AB219936

Caspase-6 (active) Staining Kit - Green Fluorescence

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(1 Publication)

Caspase 6 (active) Staining Kit - Green Fluorescence (ab219936) is a sensitive fluorometric assay to measure caspase 6 activation in live cells.
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Fluorescence Microscopy - Caspase-6 (active) Staining Kit - Green Fluorescence (AB219936)
  • Fluorescence Microscopy

Supplier Data

Fluorescence Microscopy - Caspase-6 (active) Staining Kit - Green Fluorescence (AB219936)

Active caspase 6 staining in Jurkat cells. cells (3 x 105 cells/100 μL/well) were either untreated (A) or treated with 1 μM staurosporine for 3 hours (B). Cells were incubated with FAM-VEID-FMK for 1 hour at 37°C. Increase in fluorescent intensity was observed using a fluorescence microscope with a FITC channel.

Functional Studies - Caspase-6 (active) Staining Kit - Green Fluorescence (AB219936)
  • FuncS

Supplier Data

Functional Studies - Caspase-6 (active) Staining Kit - Green Fluorescence (AB219936)

Detection of active Caspase 6 in Jurkat cells. Jurkat cells (3 x 105 cells/100 μL/well) were either untreated (control) or treated with 1 μM staurosporine for 3 hours. Cells were incubated with FAM-VEID-FMK for 1 hour at 37°C. The fluorescent signal was measured at Ex/Em = 490/525 nm (cut off at 515 nm) with a FlexStation microplate reader (Molecular Devices) using bottom read mode

Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based

Assay Platform

Microplate reader, Fluor. microscope, Flow cyt.

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Reactivity data

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Product details

Caspase 6 (active) Staining Kit - Green Fluorescence (ab219936) is a sensitive fluorometric assay to measure caspase 6 activation in live cells. The assay uses FAM-VEID-FMK, which binds irreversibly to active caspase 6 in apoptotic cells. The fluorescent intensity of the FAM-VEID-FMK signal is proportional to the amount of active caspase 6 and can be easily detected at Ex/Em = 490/525 nm by fluorescence microscopy, flow cytometer, or fluorescent microplate reader.

Caspase activity assay kits are based on fluorescent inhibitors of caspases. These inhibitors are cell permeable and non-cytotoxic. Once inside the cell, the caspase inhibitors bind covalently to the active caspases. Caspase 6 is involved in the activation cascade of caspases responsible for apoptosis execution. Overexpression of caspase 6 promotes programmed cell death. It has been proven that caspase 6 has substrate selectivity for the peptide sequence Val-Glu-Ile-Asp (VEID). This kit uses FAM-VEID-FMK as a fluorescent indicator for caspase 6 activity. FAM-VEID-FMK irreversibly binds to activated caspase 6 in apoptotic cells.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Data in brief 37:107183 PubMed34141841

2021

Cytotoxic and apoptotic data of BPA and BPA alternatives TMBPF, BPAF, and BPS in female adult rat and human stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kristen G Harnett,Ashley Chin,Sonya M Schuh
View all publications
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websiteProtocolBooklet
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