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Caspase 9 (active) FITC Staining Kit provides a convenient means for sensitive detection of activated caspase-9 in living cells.

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Key facts

Sample types

Suspension cells, Adherent cells

Assay type

Enzyme activity

Assay time

2h

Associated Products

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2 products for Alternative Product

Target data

Function

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

Alternative names

Caspase-9, CASP-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6, APAF-3, ICE-LAP6, CASP9, MCH6

What's included?

100 Test
Components
FITC-LEHD-FMK
1 x 100 µL
Wash Buffer IV
2 x 100 mL
Z-VAD-FMK
1 x 10 µL

Recommended products

Caspase 9 (active) FITC Staining Kit provides a convenient means for sensitive detection of activated caspase-9 in living cells.

Alternative names

Caspase-9, CASP-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6, APAF-3, ICE-LAP6, CASP9, MCH6

Key facts

Sample types

Suspension cells, Adherent cells

Assay type

Enzyme activity

Assay time

2h

Assay Platform

Microplate reader, Fluor. microscope, Flow cyt.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Abcam's Caspase 9 (active) FITC Staining Kit provides a convenient means for sensitive detection of activated caspase-9 in living cells. The assay utilizes the caspase-9 inhibitor LEHD-FMK conjugated to FITC (FITC-LEHD-FMK) as a fluorescent marker. FITC-LEHD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-9 in apoptotic cells. Visit our for tips and troubleshooting.

Activation of caspases plays a central role in apoptosis. **Other caspase and apoptosis assays** Review the full set of , or the .

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