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Caspase 9 (active) FITC Staining Kit provides a convenient means for sensitive detection of activated caspase-9 in living cells.

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Key facts

Sample types
Suspension cells, Adherent cells
Assay type
Enzyme activity
Assay time
2h

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Target data

Function

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates effector caspases caspase-3 (CASP3) or caspase-7 (CASP7). Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed:36758105, PubMed:36758106). Isoform 2. Lacks activity is an dominant-negative inhibitor of caspase-9.

Alternative names

MCH6, CASP9, Caspase-9, CASP-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6, APAF-3, ICE-LAP6

What's included?

100 Test
Components
FITC-LEHD-FMK
1 x 100 µL
Wash Buffer IV
2 x 100 mL
Z-VAD-FMK
1 x 10 µL

Recommended products

Caspase 9 (active) FITC Staining Kit provides a convenient means for sensitive detection of activated caspase-9 in living cells.

Key facts

Sample types
Suspension cells, Adherent cells
Assay type
Enzyme activity
Assay time
2h
Assay Platform
Microplate reader, Fluor. microscope, Flow cyt.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Abcam's Caspase 9 (active) FITC Staining Kit provides a convenient means for sensitive detection of activated caspase-9 in living cells. The assay utilizes the caspase-9 inhibitor LEHD-FMK conjugated to FITC (FITC-LEHD-FMK) as a fluorescent marker. FITC-LEHD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-9 in apoptotic cells. Visit our for tips and troubleshooting.

Activation of caspases plays a central role in apoptosis. **Other caspase and apoptosis assays** Review the full set of , or the .

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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