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AB65615

Caspase 9 (active) FITC Staining Kit

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(12 Publications)

Caspase 9 (active) FITC Staining Kit provides a convenient means for sensitive detection of activated caspase-9 in living cells.

View Alternative Names

MCH6, CASP9, Caspase-9, CASP-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6, APAF-3, ICE-LAP6

1 Images
Schematic Diagram - Caspase 9 (active) FITC Staining Kit (AB65615)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Caspase 9 (active) FITC Staining Kit (AB65615)

Representative image of Caspase 9 (active) FITC Staining Kit ab65615

Components shown from left to right :

- FITC-LEHD-FMK

- Z-VAD-FMK

- Wash Buffer IV (2 bottles)

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Sample types

Suspension cells, Adherent cells

Assay type

Enzyme activity

Assay time

2h

Assay Platform

Microplate reader, Fluor. microscope, Flow cyt.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Caspase 9 (active) FITC Staining Kit provides a convenient means for sensitive detection of activated caspase-9 in living cells. The assay utilizes the caspase-9 inhibitor LEHD-FMK conjugated to FITC (FITC-LEHD-FMK) as a fluorescent marker. FITC-LEHD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-9 in apoptotic cells. Visit our for tips and troubleshooting.

Activation of caspases plays a central role in apoptosis. **Other caspase and apoptosis assays** Review the full set of , or the .

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab65615-100Test", "assetComponentDetails": [ { "size":"2 x 100 mL", "name":"Wash Buffer IV", "number":"AB65615-CMP03", "productcode":"" }, { "size":"1 x 100 µL", "name":"FITC-LEHD-FMK", "number":"AB65615-CMP01", "productcode":"" }, { "size":"1 x 10 µL", "name":"Z-VAD-FMK", "number":"AB65615-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates effector caspases caspase-3 (CASP3) or caspase-7 (CASP7). Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed : 36758105, PubMed : 36758106).. Isoform 2. Lacks activity is an dominant-negative inhibitor of caspase-9.
See full target information CASP9
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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 28:457 PubMed39114572

2024

Metformin in combination with chemotherapy increases apoptosis in gastric cancer cells and counteracts senescence induced by chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Katia Carolina Vázquez-Ibarra,Josefina Yoaly Sánchez López,Tomás Daniel Pineda Razo,José Roberto Cruz Lozano,Brenda Guadalupe Ortiz-Tamayo,Luis Arturo Palafox-Mariscal,Rosa María González Arreola,Juan Ramón González-García,Pablo Cesar Ortiz-Lazareno

European journal of pharmacology 978:176764 PubMed38908670

2024

An 8-aminoquinoline-naphthyl copper complex causes apoptotic cell death by modulating the expression of apoptotic regulatory proteins in breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Nonzuzo Myeza,Cathy Slabber,Orde Q Munro,Sheldon Sookai,Savannah C Zacharias,Carla Martins-Furness,Leonie Harmse

Cell death discovery 9:286 PubMed37542074

2023

Cannabidiol-induced crosstalk of apoptosis and macroautophagy in colorectal cancer cells involves p53 and Hsp70.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Wang,Ali Bashiri Dezfouli,Mohammad Khosravi,Wolfgang Sievert,Stefan Stangl,Melissa Schwab,Zhiyuan Wu,Katja Steiger,Hu Ma,Gabriele Multhoff

Cancers 12: PubMed33092171

2020

Specific Effects of Trabectedin and Lurbinectedin on Human Macrophage Function and Fate-Novel Insights.

Applications

Unspecified application

Species

Unspecified reactive species

Adrián Povo-Retana,Marina Mojena,Adrian B Stremtan,Victoria B Fernández-García,Ana Gómez-Sáez,Cristina Nuevo-Tapioles,José M Molina-Guijarro,José Avendaño-Ortiz,José M Cuezva,Eduardo López-Collazo,Juan F Martínez-Leal,Lisardo Boscá

Cell reports 30:4124-4136.e5 PubMed32209473

2020

CD137 Signaling Regulates Acute Colitis via RALDH2-Expressing CD11bCD103 DCs.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Jin,In-Hyuk Jung,Shin Hye Moon,Sejin Jeon,Se-Jin Jeong,Seong-Keun Sonn,Seungwoon Seo,Mi-Ni Lee,Eun Ju Song,Hyae Yon Kweon,Sinai Kim,Tae Kyeong Kim,Juyang Kim,Hong Rae Cho,Jae-Hoon Choi,Byungsuk Kwon,Goo Taeg Oh

IUBMB life 71:1876-1895 PubMed31359611

2019

Trolox prevents high glucose-induced apoptosis in rat myocardial H9c2 cells by regulating GLUT-4 and antioxidant defense mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Ravichandra Shivalingappa Davargaon,Asha Devi Sambe,Subramanyam Muthangi V V

Molecular medicine reports 16:8721-8728 PubMed28990084

2017

Neuroprotective effect of chondroitin sulfate on SH‑SY5Y cells overexpressing wild‑type or A53T mutant α‑synuclein.

Applications

Unspecified application

Species

Unspecified reactive species

Chuanxia Ju,Jianjun Gao,Lin Hou,Lei Wang,Fang Zhang,Fusheng Sun,Tingting Zhang,Pingping Xu,Zhenyan Shi,Fang Hu,Congxiao Zhang

Oncogene 36:6863-6872 PubMed28869605

2017

Suppression of Sirt1 sensitizes lung cancer cells to WEE1 inhibitor MK-1775-induced DNA damage and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

G Chen,B Zhang,H Xu,Y Sun,Y Shi,Y Luo,H Jia,F Wang

PloS one 10:e0138607 PubMed26390405

2015

Aberrant Apoptotic Response of Colorectal Cancer Cells to Novel Nucleoside Analogues.

Applications

Unspecified application

Species

Unspecified reactive species

Leonie Harmse,Nurit Dahan-Farkas,Jenny-Lee Panayides,Willem van Otterlo,Clement Penny

Journal of leukocyte biology 96:1011-22 PubMed25225677

2014

PI3K/Akt regulates survival during differentiation of human macrophages by maintaining NF-κB-dependent expression of antiapoptotic Bcl-xL.

Applications

Unspecified application

Species

Unspecified reactive species

Aurelia Busca,Mansi Saxena,Salma Iqbal,Jonathan Angel,Ashok Kumar
View all publications
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