AB65607

Caspase 9 Assay Kit (Fluorometric)

Name: Caspase 9 Assay Kit (Fluorometric) (ab65607) | Abcam
Brand: Abcam Limited
SKU: AB65607
Price: 730 USD
Availability: InStock

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(23 Publications)

Abcam's Caspase 9 Assay Kit (Fluorometric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD.

View Alternative Names

MCH6, CASP9, Caspase-9, CASP-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6, APAF-3, ICE-LAP6

2 Images

Functional Studies - Caspase 9 Assay Kit (Fluorometric) (AB65607)

FuncS

Lab

Functional Studies - Caspase 9 Assay Kit (Fluorometric) (AB65607)

Caspase 9 activity in Jurkat cells following 24 hour exposure to 50 ng x mL^-1 anti-Fas Ab (MBL), 2 uM or 10 uM Camptothecin (CPT; ab120115) with or without 20 nM caspase inhibitor Z-VAD(OMe)-FMK (Zvad; ab120487). Sample input 5-25 uL, background signal subtracted, , duplicates; +/- SD.

Schematic Diagram - Caspase 9 Assay Kit (Fluorometric) (AB65607)

Schematic Diagram

Supplier Data

Schematic Diagram - Caspase 9 Assay Kit (Fluorometric) (AB65607)

Representative image of Caspase 9 Assay Kit (Fluorometric) ab65607

Components shown from left to right :

- LEHD-AFC

- DTT I

- 2X Reaction Buffer I (4 vials)

- Lysis Buffer IV

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Fluorescent

Sample types

Tissue Extracts, Cell Lysate

Assay type

Enzyme activity

Assay time

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's Caspase 9 Assay Kit (Fluorometric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD. The assay is based on detection of cleavage of substrate LEHD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEHD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by caspase-9 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or fluorecence microtiter plate reader.

This product is manufactured by BioVision, an Abcam company and was previously called K118 Caspase-9 Fluorometric Assay Kit. K118-100 is the same size as the 100 test size of ab65607.

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.

Other caspase and apoptosis assays

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab65607-100Test", "assetComponentDetails": [ { "size":"1 x 400 µL", "name":"DTT I", "number":"AB65607-CMP03", "productcode":"" }, { "size":"4 x 2 mL", "name":"2X Reaction Buffer I", "number":"AB65607-CMP01", "productcode":"" }, { "size":"1 x 100 mL", "name":"Lysis Buffer IV", "number":"AB65607-CMP04", "productcode":"" }, { "size":"1 x 0.5 mL", "name":"LEHD-AFC", "number":"AB65607-CMP02", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Product protocols

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Target data

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates effector caspases caspase-3 (CASP3) or caspase-7 (CASP7). Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed : 36758105, PubMed : 36758106).. Isoform 2. Lacks activity is an dominant-negative inhibitor of caspase-9.

See full target information CASP9

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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Journal of lipid research 64:100444 PubMed37730163

2023

New role for the anandamide metabolite prostaglandin F ethanolamide: Rolling preadipocyte proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Besma Boubertakh,Olivier Courtemanche,David Marsolais,Vincenzo Di Marzo,Cristoforo Silvestri

Scientific reports 13:5370 PubMed37005457

2023

Pyrazole derivatives of pyridine and naphthyridine as proapoptotic agents in cervical and breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rima D Alharthy,Faisal Rashid,Abida Ashraf,Zahid Shafiq,Steven Ford,Mariya Al-Rashida,Muhammad Yaqub,Jamshed Iqbal

Food chemistry. Molecular sciences 5:100131 PubMed36060473

2022

Comparison of oxidative stress-mitochondria-mediated tenderization in two different bovine muscles during aging.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenjiang Ding,Qichao Wei,Chunmei Liu,Chunhui Zhang,Feng Huang

Cancer medicine 12:4455-4471 PubMed35946957

2022

βIII-tubulin suppression enhances the activity of Amuvatinib to inhibit cell proliferation in c-Met positive non-small cell lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Simon Brayford,Alastair Duly,Wee Siang Teo,Tanya Dwarte,Estrella Gonzales-Aloy,Zerong Ma,Laura McVeigh,Timothy W Failes,Greg M Arndt,Joshua A McCarroll,Maria Kavallaris

Hepatology communications 6:2354-2367 PubMed35621045

2022

The unfolded protein response to PI*Z alpha-1 antitrypsin in human hepatocellular and murine models.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanqing Lu,Liqun R Wang,Jungnam Lee,Naweed S Mohammad,Alek M Aranyos,Calvin Gould,Nazli Khodayari,Regina A Oshins,Craig G Moneypenny,Mark L Brantly

Science advances 7:eabi8051 PubMed34739325

2021

Genome-wide association studies identify the role of caspase-9 in kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Tomohito Doke,Shizheng Huang,Chengxiang Qiu,Xin Sheng,Matthew Seasock,Hongbo Liu,Ziyuan Ma,Matthew Palmer,Katalin Susztak

Cell death & disease 11:483 PubMed32587235

2020

A small-molecule ARTS mimetic promotes apoptosis through degradation of both XIAP and Bcl-2.

Applications

Unspecified application

Species

Unspecified reactive species

Dana Mamriev,Ruqaia Abbas,Franca-Maria Klingler,Juliana Kagan,Nir Kfir,Alastair Donald,Keren Weidenfeld,David W Sheppard,Dalit Barkan,Sarit Larisch

Cell reports 31:107731 PubMed32521256

2020

Failed Apoptosis Enhances Melanoma Cancer Cell Aggressiveness.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin Berthenet,Camila Castillo Ferrer,Deborah Fanfone,Nikolay Popgeorgiev,David Neves,Philippe Bertolino,Benjamin Gibert,Hector Hernandez-Vargas,Gabriel Ichim

Neurotoxicity research 37:743-752 PubMed31997155

2020

Some Ototoxic Drugs Destroy Cochlear Support Cells Before Damaging Sensory Hair Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dalian Ding,Jianhui Zhang,Haiyan Jiang,Weijun Xuan,Weidong Qi,Richard Salvi

Marine drugs 15: PubMed29125578

2017

An Insight into Sargassum muticum Cytoprotective Mechanisms against Oxidative Stress on a Human Cell In Vitro Model.

Applications

Unspecified application

Species

Unspecified reactive species

Susete Pinteus,Marco F L Lemos,Joana Silva,Celso Alves,Agnieszka Neugebauer,Rafaela Freitas,Adriana Duarte,Rui Pedrosa

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Caspase 9 Assay Kit (Fluorometric)

Functional Studies - Caspase 9 Assay Kit (Fluorometric) (AB65607)

Schematic Diagram - Caspase 9 Assay Kit (Fluorometric) (AB65607)

Key facts

Detection method

Sample types

Assay type

Assay time

Assay Platform

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (23)

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Product promise