Abcam's Caspase 9 Assay Kit (Fluorometric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD.
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- Scientific reports 13:53702023Pyrazole derivatives of pyridine and naphthyridine as proapoptotic agents in cervical and breast cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRima D Alharthy,Faisal Rashid,Abida Ashraf,Zahid Shafiq,Steven Ford,Mariya Al-Rashida,Muhammad Yaqub,Jamshed IqbalPubMed 37005457
- Food chemistry. Molecular sciences 5:1001312022Comparison of oxidative stress-mitochondria-mediated tenderization in two different bovine muscles during aging.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenjiang Ding,Qichao Wei,Chunmei Liu,Chunhui Zhang,Feng HuangPubMed 36060473
- Cancer medicine 12:4455-44712022βIII-tubulin suppression enhances the activity of Amuvatinib to inhibit cell proliferation in c-Met positive non-small cell lung cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSimon Brayford,Alastair Duly,Wee Siang Teo,Tanya Dwarte,Estrella Gonzales-Aloy,Zerong Ma,Laura McVeigh,Timothy W Failes,Greg M Arndt,Joshua A McCarroll,Maria KavallarisPubMed 35946957
- Hepatology communications 6:2354-23672022The unfolded protein response to PI*Z alpha-1 antitrypsin in human hepatocellular and murine models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuanqing Lu,Liqun R Wang,Jungnam Lee,Naweed S Mohammad,Alek M Aranyos,Calvin Gould,Nazli Khodayari,Regina A Oshins,Craig G Moneypenny,Mark L BrantlyPubMed 35621045
- Science advances 7:eabi80512021Genome-wide association studies identify the role of caspase-9 in kidney disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomohito Doke,Shizheng Huang,Chengxiang Qiu,Xin Sheng,Matthew Seasock,Hongbo Liu,Ziyuan Ma,Matthew Palmer,Katalin SusztakPubMed 34739325
- Cell death & disease 11:4832020A small-molecule ARTS mimetic promotes apoptosis through degradation of both XIAP and Bcl-2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDana Mamriev et. al.PubMed 32587235
- Cell reports 31:1077312020Failed Apoptosis Enhances Melanoma Cancer Cell Aggressiveness.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKevin Berthenet,Camila Castillo Ferrer,Deborah Fanfone,Nikolay Popgeorgiev,David Neves,Philippe Bertolino,Benjamin Gibert,Hector Hernandez-Vargas,Gabriel IchimPubMed 32521256
- Neurotoxicity research 37:743-7522020Some Ototoxic Drugs Destroy Cochlear Support Cells Before Damaging Sensory Hair Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDalian Ding et. al.PubMed 31997155
- Marine drugs 15:2017An Insight into Sargassum muticum Cytoprotective Mechanisms against Oxidative Stress on a Human Cell In Vitro Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSusete Pinteus,Marco F L Lemos,Joana Silva,Celso Alves,Agnieszka Neugebauer,Rafaela Freitas,Adriana Duarte,Rui PedrosaPubMed 29125578
- Molecular medicine reports 16:6626-66332017N‑acetylcysteine induces apoptosis via the mitochondria‑dependent pathway but not via endoplasmic reticulum stress in H9c2 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuyong Liu,Ke Liu,Nian Wang,Huali ZhangPubMed 28901511
Key facts
- Detection method
Fluorescent
- Sample types
Tissue Extracts, Cell Lysate
- Assay type
Enzyme activity
- Assay time
2h
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Target data
Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates effector caspases caspase-3 (CASP3) or caspase-7 (CASP7). Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed:36758105, PubMed:36758106).Isoform 2Lacks activity is an dominant-negative inhibitor of caspase-9.
Alternative names
MCH6, CASP9, MCH6, Caspase-9, CASP-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6, APAF-3, ICE-LAP6
What's included?
Recommended products
Abcam's Caspase 9 Assay Kit (Fluorometric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD.
Key facts
- Detection method
Fluorescent
- Sample types
Tissue Extracts, Cell Lysate
- Assay type
Enzyme activity
- Assay time
2h
- Assay Platform
Microplate reader
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Abcam's Caspase 9 Assay Kit (Fluorometric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD. The assay is based on detection of cleavage of substrate LEHD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEHD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by caspase-9 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or fluorecence microtiter plate reader.
This product is manufactured by BioVision, an Abcam company and was previously called K118 Caspase-9 Fluorometric Assay Kit. K118-100 is the same size as the 100 test size of ab65607.
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.
Other caspase and apoptosis assays
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Functional Studies - Caspase 9 Assay Kit (Fluorometric) (ab65607)
Caspase 9 activity in Jurkat cells following 24 hour exposure to 50 ng x mL-1 anti-Fas Ab (MBL), 2 uM or 10 uM Camptothecin (CPT; Camptothecin, DNA topoisomerase inhibitor ab120115) with or without 20 nM caspase inhibitor Z-VAD(OMe)-FMK (Zvad; Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor ab120487). Sample input 5-25 uL, background signal subtracted, , duplicates; +/- SD.
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