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AB270770

Caspase Assay Kit (orange, VAD-FMK)

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(1 Publication)

Caspases play important roles in apoptosis and inflammation.

Key facts

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Reactivity data

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Product details

Caspases play important roles in apoptosis and inflammation. Caspase Assay Kit (orange, VAD-FMK) (ab270770) is used by researchers seeking to quantitate apoptosis via caspase activity in cultured cells and tissues.

The FLICA reagent SR-VAD-FMK enters each cell and irreversibly binds to activated caspases. Because the SR-VAD-FMK FLICA reagent becomes covalently coupled to the active enzymes, it is retained within the cell, while any unbound SR-VAD-FMK FLICA reagent diffuses out of the cell and is washed away. The remaining red fluorescent signal is a direct measure of the active caspase enzyme activity present in the cell at the time the reagent was added. Cells that contain the bound FLICA can be analyzed by a fluorescence plate reader, fluorescence microscopy or flow cytometry. Cells labeled with the FLICA reagent may be read immediately or preserved for 16 hours using the fixative included in the kit. Unfixed samples may also be analyzed with Hoechst 33342 to detect changes in nuclear morphology.

Produced using a non-baculovirus expression system.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:663654 PubMed34178988

2021

LncRNA HCP5 Induces Gastric Cancer Cell Proliferation, Invasion, and EMT Processes Through the miR-186-5p/WNT5A Axis Under Hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Gao,Liying Liu,Yudan Yang,Mengyi Li,Qingqing Ma,Zhiwei Chang
View all publications
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Product promise

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