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Catalase Activity Assay Kit (Colorimetric/Fluorometric) (ab83464) is a highly sensitive, simple and direct assay for measuring catalase activity in a variety of biological samples such as cell and tissue lysates or biological fluids.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.


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Functional Studies - Catalase Activity Assay Kit (Colorimetric/Fluorometric) (AB83464), expandable thumbnail
  • Functional Studies - Catalase Activity Assay Kit (Colorimetric/Fluorometric) (AB83464), expandable thumbnail

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Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Tissue Lysate, Plasma, Serum, Cell Lysate

Assay type

Enzyme activity (quantitative)

Reactive species

Mammals

Assay time

40m

Associated Products

Select an associated product type

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Target data

Function

Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.

Alternative names

What's included?

2000 Test
Components
Assay Buffer VIII
20 x 25 mL
Catalase Positive Control
20 x 20 µL
Developer Solution V
20 x 1 Vial
Hydrogen Peroxide Solution III
20 x 25 µL
OxiRed Probe
20 x 200 µL
Stop Solution III
20 x 1 mL

Recommended products

Catalase Activity Assay Kit (Colorimetric/Fluorometric) (ab83464) is a highly sensitive, simple and direct assay for measuring catalase activity in a variety of biological samples such as cell and tissue lysates or biological fluids.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Alternative names

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Tissue Lysate, Plasma, Serum, Cell Lysate

Assay type

Enzyme activity (quantitative)

Reactive species

Mammals

Assay time

40m

Assay Platform

Microplate reader

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Catalase Activity Assay Kit (Colorimetric/Fluorometric) (ab83464) is a highly sensitive, simple and direct assay for measuring catalase activity in a variety of biological samples such as cell and tissue lysates or biological fluids.

In the catalase activity assay protocol, the catalase present in the sample reacts with hydrogen peroxide (H2O2) to produce water and oxygen. The unconverted H2O2 reacts with probe to produce a product that can be measured colorimetrically at OD 570 nm or fluorometrically at Ex/Em = 535/587 nm. Therefore, the catalase activity present in the sample is reversely proportional to the signal obtained. The kit can detect as little as 1 μU of catalase activity.

Catalase activity assay protocol summary:
- add samples and standards to wells
- add stop solution into sample control wells and incubate at 25°C for 5 min
- add H2O2 solution into wells and incubate for 30 min at 25°C
- add stop solution
- add developer mix and incubate for 10 min at 25°C
- analyze with microplate reader

Technical Note: This assay is not recommended for use in urine samples due to the presense of reducing agents.

This product is manufactured by BioVision, an Abcam company and was previously called K773 Catalase Activity Colorimetric/Fluorometric Assay Kit. K773-100 is the same size as the 100 test size of ab83464.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Catalase also known as CAT is an enzyme that catalyzes the decomposition of hydrogen peroxide into water and oxygen. This enzyme has a molecular weight of approximately 240 kDa and typically forms a tetramer. Catalase mainly resides in peroxisomes functioning as a peroxisome marker. It expresses abundantly in the liver kidneys and erythrocytes where it plays significant roles in cellular protection against oxidative damage. The presence of Catalase makes it an ideal candidate for use in peroxisome staining and peroxisome image analysis in research.

Biological function summary

Catalase contributes to antioxidant defense by breaking down hydrogen peroxide preventing cellular damage. In peroxisomes it works alongside other peroxisomal enzymes to maintain cell health and metabolic regulation. Catalase does not form a complex but interacts closely with other enzymes like superoxide dismutase which dismutates superoxide radicals into less harmful substances. Increased Catalase activity levels can be measured using Catalase activity kits and activity assays allowing us to learn about peroxisome function.

Pathways

Hydrogen peroxide removal by Catalase is vital in the reactive oxygen species (ROS) metabolic process and plays a part in the cellular response to oxidative stress. Catalase interacts with the glutathione peroxidase pathway safeguarding cells from oxidative stress-related damage. Superoxide dismutase works synergistically with Catalase transforming superoxide anions into hydrogen peroxide before its decomposition by Catalase. These activities highlight the essential role Catalase plays in protecting cells from oxidative stress damage.

Associated diseases and disorders

Catalase relates to conditions like acatalasemia and diabetes. Acatalasemia a condition caused by a deficiency of Catalase increases the risk of developing diabetes and other oxidative stress-related diseases. Mutations in the CAT gene can lead to decreased Catalase activity contributing to the onset of these conditions. Additionally Catalase works with other proteins like glutathione peroxidase in mitigating the effects of oxidative stress with deficiencies potentially exacerbating complications in diabetes management.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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2 product images

  • Functional Studies - Catalase Activity Assay Kit (Colorimetric/Fluorometric) (ab83464), expandable thumbnail

    Functional Studies - Catalase Activity Assay Kit (Colorimetric/Fluorometric) (ab83464)

    Sample tests using ab83464.

  • Functional Studies - Catalase Activity Assay Kit (Colorimetric/Fluorometric) (ab83464), expandable thumbnail

    Functional Studies - Catalase Activity Assay Kit (Colorimetric/Fluorometric) (ab83464)

    H2O2 standard curve using ab83464.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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