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AB83464

Catalase Activity Assay Kit (Colorimetric/Fluorometric)

4

(3 Reviews)

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(133 Publications)

Catalase Activity Assay Kit (Colorimetric/Fluorometric) (ab83464) is a highly sensitive, simple and direct assay for measuring catalase activity in a variety of biological samples such as cell and tissue lysates or biological fluids. Readout on any colorimetric (570 nm) or fluorometric (Ex/Em 535/587 nm) plate reader.

- Cited in > 120 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

Catalase, CAT

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Functional Studies - Catalase Activity Assay Kit (Colorimetric/Fluorometric) (AB83464)
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Supplier Data

Functional Studies - Catalase Activity Assay Kit (Colorimetric/Fluorometric) (AB83464)

H2O2 standard curve using ab83464.

Functional Studies - Catalase Activity Assay Kit (Colorimetric/Fluorometric) (AB83464)
  • FuncS

Supplier Data

Functional Studies - Catalase Activity Assay Kit (Colorimetric/Fluorometric) (AB83464)

Sample tests using ab83464.

Other - Catalase Activity Assay Kit (Colorimetric/Fluorometric) (AB83464)
  • Other

Supplier Data

Other - Catalase Activity Assay Kit (Colorimetric/Fluorometric) (AB83464)

Representative image of Catalase Activity Assay Kit (Colorimetric/Fluorometric) ab83464

Components shown from left to right :

- OxiRed Probe

- Hydrogen Peroxide Solution III

- Catalase Positive Control

- Developer Solution V

- Assay Buffer 8

- Stop Solution III

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Tissue Lysate, Plasma, Serum, Cell Lysate

Assay type

Enzyme activity

Results type

Quantitative

Assay time

40m

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Catalase Activity Assay Kit (Colorimetric/Fluorometric) (ab83464) is a highly sensitive, simple and direct assay for measuring catalase activity in a variety of biological samples such as cell and tissue lysates or biological fluids.

In the catalase activity assay protocol, the catalase present in the sample reacts with hydrogen peroxide (H2O2) to produce water and oxygen. The unconverted H2O2 reacts with probe to produce a product that can be measured colorimetrically at OD 570 nm or fluorometrically at Ex/Em = 535/587 nm. Therefore, the catalase activity present in the sample is reversely proportional to the signal obtained. The kit can detect as little as 1 μU of catalase activity.

Catalase activity assay protocol summary:
- add samples and standards to wells
- add stop solution into sample control wells and incubate at 25°C for 5 min
- add H2O2 solution into wells and incubate for 30 min at 25°C
- add stop solution
- add developer mix and incubate for 10 min at 25°C
- analyze with microplate reader

Technical Note: This assay is not recommended for use in urine samples due to the presense of reducing agents.

This product is manufactured by BioVision, an Abcam company and was previously called K773 Catalase Activity Colorimetric/Fluorometric Assay Kit. K773-100 is the same size as the 100 test size of ab83464.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "2000Test": { "sellingSize": "2000 Test", "publicAssetCode":"ab83464-2000Test", "assetComponentDetails": [ { "size":"20 x 1 mL", "name":"Stop Solution III", "number":"AB83464-CMP06" }, { "size":"20 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB83464-CMP05" }, { "size":"20 x 25 µL", "name":"Hydrogen Peroxide Solution III", "number":"AB83464-CMP01" }, { "size":"20 x 1 Vial", "name":"Developer Solution V", "number":"AB83464-CMP02" }, { "size":"20 x 20 µL", "name":"Catalase Positive Control", "number":"AB83464-CMP04" }, { "size":"20 x 25 mL", "name":"Assay Buffer 8", "number":"AB83464-CMP03" } ] }, "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab83464-100Test", "assetComponentDetails": [ { "size":"1 x 1 mL", "name":"Stop Solution III", "number":"AB83464-CMP06", "productcode":"" }, { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB83464-CMP05", "productcode":"AB309437" }, { "size":"1 x 25 µL", "name":"Hydrogen Peroxide Solution III", "number":"AB83464-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Solution V", "number":"AB83464-CMP02", "productcode":"" }, { "size":"1 x 20 µL", "name":"Catalase Positive Control", "number":"AB83464-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 8", "number":"AB83464-CMP03", "productcode":"AB323941" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Catalase also known as CAT is an enzyme that catalyzes the decomposition of hydrogen peroxide into water and oxygen. This enzyme has a molecular weight of approximately 240 kDa and typically forms a tetramer. Catalase mainly resides in peroxisomes functioning as a peroxisome marker. It expresses abundantly in the liver kidneys and erythrocytes where it plays significant roles in cellular protection against oxidative damage. The presence of Catalase makes it an ideal candidate for use in peroxisome staining and peroxisome image analysis in research.
Biological function summary

Catalase contributes to antioxidant defense by breaking down hydrogen peroxide preventing cellular damage. In peroxisomes it works alongside other peroxisomal enzymes to maintain cell health and metabolic regulation. Catalase does not form a complex but interacts closely with other enzymes like superoxide dismutase which dismutates superoxide radicals into less harmful substances. Increased Catalase activity levels can be measured using Catalase activity kits and activity assays allowing us to learn about peroxisome function.

Pathways

Hydrogen peroxide removal by Catalase is vital in the reactive oxygen species (ROS) metabolic process and plays a part in the cellular response to oxidative stress. Catalase interacts with the glutathione peroxidase pathway safeguarding cells from oxidative stress-related damage. Superoxide dismutase works synergistically with Catalase transforming superoxide anions into hydrogen peroxide before its decomposition by Catalase. These activities highlight the essential role Catalase plays in protecting cells from oxidative stress damage.

Catalase relates to conditions like acatalasemia and diabetes. Acatalasemia a condition caused by a deficiency of Catalase increases the risk of developing diabetes and other oxidative stress-related diseases. Mutations in the CAT gene can lead to decreased Catalase activity contributing to the onset of these conditions. Additionally Catalase works with other proteins like glutathione peroxidase in mitigating the effects of oxidative stress with deficiencies potentially exacerbating complications in diabetes management.
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Product protocols

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Target data

Catalyzes the degradation of hydrogen peroxide (H(2)O(2)) generated by peroxisomal oxidases to water and oxygen, thereby protecting cells from the toxic effects of hydrogen peroxide (PubMed : 7882369). Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells (PubMed : 7882369).
See full target information Catalase
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Publications (133)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 32: PubMed40539450

2025

Protective effects of lipid mediators, obtained from docosahexaenoic acid via soybean lipoxygenase, on lipopolysaccharide‑induced acute lung injury through the NF‑κB and Nrf2/HO‑1 signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Su,Hack Sun Choi,Soon Kyu Kwon,Yunjon Han,Soon-Chang Cho,Jin Hyuk Shin,Yong-Suk Jang,Jong Hyun Choi,Jeong-Woo Seo

Scientific reports 15:10804 PubMed40155664

2025

Hepatotoxicity evaluation method through multiple-factor analysis using human pluripotent stem cell derived hepatic organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Dae-Seop Shin,Jung Yoon Yang,Ha Neul Jeong,Seon Ju Mun,Hyunwoo Kim,Myung Jin Son,Myung Ae Bae

Annals of translational medicine 13:1 PubMed40115065

2025

Amniotic fluid stem cell conditioned medium's role in Schwann cell proliferation, survival, and cellular antioxidant activity under normative and oxidative stress conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Chukwuweike Gwam,Nequesha Mohamed,Ayobami S Ogunsola,Marcel G Brown,Kaitlin A Henry,Xue Ma

Preventive nutrition and food science 30:47-55 PubMed40059920

2025

Standardized Fingerroot () Extract Decelerates the Development of Sarcopenia in Aged Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Changhee Kim,Minseong Kang,Yeeun Kim,Jae-Kwan Hwang

Antioxidants (Basel, Switzerland) 14: PubMed39857423

2025

Shrimp White Spot Viral Infections Are Attenuated by Organic Acids by Regulating the Expression of HO-1 Oxygenase and β-1,3-Glucan-Binding Protein.

Applications

Unspecified application

Species

Unspecified reactive species

Ioan Pet,Igori Balta,Nicolae Corcionivoschi,Tiberiu Iancu,Ducu Stef,Lavinia Stef,Iuliana Cretescu

Redox biology 77:103405 PubMed39490313

2024

Disruption of canonical AHR-mediated induction of hepatocyte PKM2 expression compromises antioxidant defenses and increases TCDD-induced hepatotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Karina Orlowska,Rance Nault,Jesmin Ara,John J LaPres,Jack Harkema,Elena Y Demireva,Huirong Xie,Rachel H Wilson,Christopher A Bradfield,Dianne Yap,Aditya Joshi,Cornelis J Elferink,Tim Zacharewski

Antioxidants (Basel, Switzerland) 13: PubMed39456521

2024

Astaxanthin Supplementation Effects in Right Ventricle of Rats Exposed to Chronic Intermittent Hypobaric Hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Eduardo Pena,Samia El Alam,Constanza Gonzalez,Isaac Cortés,Diego Aguilera,Karen Flores,Karem Arriaza

Nutrients 16: PubMed39339761

2024

Exploring the Wound Healing Potential of Hispidin.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Shan Liu,Mei-Chou Lai,Tang-Yao Hong,I-Min Liu

Pharmaceuticals (Basel, Switzerland) 17: PubMed39204180

2024

Novel Insights into Amlodipine-Induced Gingival Enlargement: A Clinical and Molecular Perspective.

Applications

Unspecified application

Species

Unspecified reactive species

Jana Mojsilović,Nemanja Jovičić,Sanja Vujović Ristić,Momir Stevanović,Sara Mijailović,Gvozden Rosić,Slobodan Janković,Marina Kostić

BMC oral health 24:892 PubMed39098894

2024

"The effects of non-surgical periodontal treatment plus zinc and magnesium supplementation on oxidative stress and antioxidants enzymes in type 2 diabetes patients: a quasi-experimental study".

Applications

Unspecified application

Species

Unspecified reactive species

José Carlos Alarcón-Moreno,Mónica Flores-Muñoz,María Sobeida L Blázquez-Morales,Miguel Eric García-Rivera,Juan Carlos Rodríguez-Alba,Claudio Rafael Castro-López,Francisco Javier Nachón-García,Víctor Hugo Muñoz-Muñoz,María Gabriela Nachón-García
View all publications
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Product promise

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