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Catalase Activity Assay Kit (Colorimetric/Fluorometric) (ab83464) is a highly sensitive, simple and direct assay for measuring catalase activity in a variety of biological samples such as cell and tissue lysates or biological fluids.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

Detection method

Colorimetric/Fluorometric

Sample types

Tissue Lysate, Urine, Plasma, Serum, Cell Lysate

Assay type

Enzyme activity (quantitative)

Reactive species

Mammals

Assay time

40m

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Images

Target data

What's included?

2000 Test
Components
Assay Buffer VIII
20 x 25 mL
Catalase Positive Control
20 x 20 µL
Developer Solution V
20 x 1 Vial
Hydrogen Peroxide Solution III
20 x 25 µL
OxiRed Probe
20 x 200 µL
Stop Solution III
20 x 1 mL

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Catalase Activity Assay Kit (Colorimetric/Fluorometric) (ab83464) is a highly sensitive, simple and direct assay for measuring catalase activity in a variety of biological samples such as cell and tissue lysates or biological fluids.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Tissue Lysate, Urine, Plasma, Serum, Cell Lysate

Assay type

Enzyme activity (quantitative)

Reactive species

Mammals

Assay time

40m

Assay Platform

Microplate reader

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Catalase Activity Assay Kit (Colorimetric/Fluorometric) (ab83464) is a highly sensitive, simple and direct assay for measuring catalase activity in a variety of biological samples such as cell and tissue lysates or biological fluids.

In the catalase activity assay protocol, the catalase present in the sample reacts with hydrogen peroxide (H2O2) to produce water and oxygen. The unconverted H2O2 reacts with probe to produce a product that can be measured colorimetrically at OD 570 nm or fluorometrically at Ex/Em = 535/587 nm. Therefore, the catalase activity present in the sample is reversely proportional to the signal obtained. The kit can detect as little as 1 μU of catalase activity.

Catalase activity assay protocol summary:
- add samples and standards to wells
- add stop solution into sample control wells and incubate at 25°C for 5 min
- add H2O2 solution into wells and incubate for 30 min at 25°C
- add stop solution
- add developer mix and incubate for 10 min at 25°C
- analyze with microplate reader

This product is manufactured by BioVision, an Abcam company and was previously called K773 Catalase Activity Colorimetric/Fluorometric Assay Kit. K773-100 is the same size as the 100 test size of ab83464.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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