Catalase Activity Assay Kit (Colorimetric/Fluorometric) (ab83464) is a highly sensitive, simple and direct assay for measuring catalase activity in a variety of biological samples such as cell and tissue lysates or biological fluids.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Chemical biology & drug design 103:e144742024Hydromorphone hydrochloride preconditioning combined with postconditioning attenuates myocardial ischemia/reperfusion injury in rats by improving mitochondrial function and activating the PI3K/Akt signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiuji Qiu,Yan Yan,Guocheng Zhong,Zhiqi Hou,Yongcai Ye,Jiaying Lin,Dexing LuoPubMed 38342769
- Redox biology 67:1029312023The SGK3-Catalase antioxidant signaling axis drives cervical cancer growth and therapy resistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Wang,Jiannan Liu,Xingming Liao,Yasong Yi,Yijue Xue,Ling Yang,Hailing Cheng,Pixu LiuPubMed 37866161
- Annals of medicine 55:22589172023Cytotoxic effects of dose dependent inorganic magnesium oxide nanoparticles on the reproductive organs of rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGhada H Naguib,Gamal S Abd El-Aziz,Rayyan A Kayal,Abdulghani I Mira,Maher S Hajjaj,Hisham A Mously,Mohamed T HamedPubMed 37769030
- Journal of cellular and molecular medicine 27:3189-32012023Hyperbaric oxygen therapy suppresses hypoxia and reoxygenation injury to retinal pigment epithelial cells through activating peroxisome proliferator activator receptor-alpha signalling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTzong-Bor Sun,Kuo-Feng Huang,Ko-Chi Niu,Cheng-Hsien Lin,Wen-Pin Liu,Chao-Hung Yeh,Shu-Chun Kuo,Ching-Ping ChangPubMed 37731202
- BMC zoology 8:212023Evaluation of freshwater heavy metals accumulation effect on oxidative stress, Metallothionein biosynthesis and histopathology of Procambarus clarkii (Girard,1985) collected from three locations in the Delta region, Egypt.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMahy M Mona,Mai L Younis,Aalaa I AtlamPubMed 37718412
- Arthritis research & therapy 25:1262023Deubiquitinase USP16 induces gouty arthritis via Drp1-dependent mitochondrial fission and NLRP3 inflammasome activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingdong Wang,Hongbin QiuPubMed 37488647
- Pharmaceutics 15:2023Natural Antimicrobials Block the Host NF-κB Pathway and Reduce Infection Both In Vitro and In Vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIulia Adelina Bunduruș,Igori Balta,Eugenia Butucel,Todd Callaway,Cosmin Alin Popescu,Tiberiu Iancu,Ioan Pet,Lavinia Stef,Nicolae CorcionivoschiPubMed 37514180
- Molecules (Basel, Switzerland) 28:2023Phenolics Extracted from Mitigates Diabetic Cardiomyopathy by Modulating Oxidative Stress, Apoptotic Mediators and the Nfr-2/HO-1 Pathway in Alloxan-Induced Diabetic Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesUrooj Umar,Sibtain Ahmed,Asra Iftikhar,Maryam Iftikhar,Wafa Majeed,Atika Liaqat,Sana Shahzad,Mateen Abbas,Tahir Mehmood,Farooq AnwarPubMed 37513325
- Redox biology 64:1027802023Leucine zipper protein 1 prevents doxorubicin-induced cardiotoxicity in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDi Fan,Zhili Jin,Jianlei Cao,Yi Li,Tao He,Wei Zhang,Li Peng,Huixia Liu,Xiaoyan Wu,Ming Chen,Yongzhen Fan,Bo He,Wenxi Yu,Hairong Wang,Xiaorong Hu,Zhibing LuPubMed 37354826
- Antioxidants (Basel, Switzerland) 12:2023Effect of Crocetin on Basal Lipolysis in 3T3-L1 Adipocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrancisco J Cimas,Miguel Ángel De la Cruz-Morcillo,Carmen Cifuentes,Natalia Moratalla-López,Gonzalo L Alonso,Eduardo Nava,Sílvia LlorensPubMed 37371984
Key facts
- Detection method
Colorimetric/Fluorometric
- Sample types
Tissue Lysate, Plasma, Serum, Cell Lysate
- Assay type
Enzyme activity (quantitative)
- Reactive species
Mammals
- Assay time
40m
Associated Products
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Target data
Function
Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
Alternative names
Catalase, CAT
What's included?
Recommended products
Catalase Activity Assay Kit (Colorimetric/Fluorometric) (ab83464) is a highly sensitive, simple and direct assay for measuring catalase activity in a variety of biological samples such as cell and tissue lysates or biological fluids.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Alternative names
Catalase, CAT
Key facts
- Detection method
Colorimetric/Fluorometric
- Sample types
Tissue Lysate, Plasma, Serum, Cell Lysate
- Assay type
Enzyme activity (quantitative)
- Reactive species
Mammals
- Assay time
40m
- Assay Platform
Microplate reader
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Catalase Activity Assay Kit (Colorimetric/Fluorometric) (ab83464) is a highly sensitive, simple and direct assay for measuring catalase activity in a variety of biological samples such as cell and tissue lysates or biological fluids.
In the catalase activity assay protocol, the catalase present in the sample reacts with hydrogen peroxide (H2O2) to produce water and oxygen. The unconverted H2O2 reacts with probe to produce a product that can be measured colorimetrically at OD 570 nm or fluorometrically at Ex/Em = 535/587 nm. Therefore, the catalase activity present in the sample is reversely proportional to the signal obtained. The kit can detect as little as 1 μU of catalase activity.
Catalase activity assay protocol summary:
- add samples and standards to wells
- add stop solution into sample control wells and incubate at 25°C for 5 min
- add H2O2 solution into wells and incubate for 30 min at 25°C
- add stop solution
- add developer mix and incubate for 10 min at 25°C
- analyze with microplate reader
Technical Note: This assay is not recommended for use in urine samples due to the presense of reducing agents.
This product is manufactured by BioVision, an Abcam company and was previously called K773 Catalase Activity Colorimetric/Fluorometric Assay Kit. K773-100 is the same size as the 100 test size of ab83464.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Catalase also known as CAT is an enzyme that catalyzes the decomposition of hydrogen peroxide into water and oxygen. This enzyme has a molecular weight of approximately 240 kDa and typically forms a tetramer. Catalase mainly resides in peroxisomes functioning as a peroxisome marker. It expresses abundantly in the liver kidneys and erythrocytes where it plays significant roles in cellular protection against oxidative damage. The presence of Catalase makes it an ideal candidate for use in peroxisome staining and peroxisome image analysis in research.
- Biological function summary
Catalase contributes to antioxidant defense by breaking down hydrogen peroxide preventing cellular damage. In peroxisomes it works alongside other peroxisomal enzymes to maintain cell health and metabolic regulation. Catalase does not form a complex but interacts closely with other enzymes like superoxide dismutase which dismutates superoxide radicals into less harmful substances. Increased Catalase activity levels can be measured using Catalase activity kits and activity assays allowing us to learn about peroxisome function.
- Pathways
Hydrogen peroxide removal by Catalase is vital in the reactive oxygen species (ROS) metabolic process and plays a part in the cellular response to oxidative stress. Catalase interacts with the glutathione peroxidase pathway safeguarding cells from oxidative stress-related damage. Superoxide dismutase works synergistically with Catalase transforming superoxide anions into hydrogen peroxide before its decomposition by Catalase. These activities highlight the essential role Catalase plays in protecting cells from oxidative stress damage.
- Associated diseases and disorders
Catalase relates to conditions like acatalasemia and diabetes. Acatalasemia a condition caused by a deficiency of Catalase increases the risk of developing diabetes and other oxidative stress-related diseases. Mutations in the CAT gene can lead to decreased Catalase activity contributing to the onset of these conditions. Additionally Catalase works with other proteins like glutathione peroxidase in mitigating the effects of oxidative stress with deficiencies potentially exacerbating complications in diabetes management.
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2 product images
Functional Studies - Catalase Activity Assay Kit (Colorimetric/Fluorometric) (ab83464)
Sample tests using ab83464.
Functional Studies - Catalase Activity Assay Kit (Colorimetric/Fluorometric) (ab83464)
H2O2 standard curve using ab83464.
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