Cathepsin B Activity Assay Kit (Fluorometric)

• FuncS

Lab

Functional Studies - Cathepsin B Activity Assay Kit (Fluorometric) (AB65300)

Cathepsin B activity in HL60 cell lysates (2.5x10e6 cells) with the addition of inhibitor.

• FuncS

PubMed

Functional Studies - Cathepsin B Activity Assay Kit (Fluorometric) (AB65300)

Functional studies - Cathespin B Activity Assay Kit.

Enzyme activity of Cathepsin B and Cathepsin D was determined using Cathepsin B/D activity assay kit (ab65300 and ab65302). Neuro2a cell lysates were centrifuged at 10,000 xg for 10 minutes at 4°C and supernatant was used for the assay. Cathepsin activity was performed at the 24 and 48 hours time points by cleavage of the fluorescence peptide substrate [DnP-DR-MCA, GKPILFFRLK(DnP)-DR substrate peptide labeled with MCA]. Data represent the mean ± SD (*p<0.05, **p<0.001).

Bong G.J., PLoS One 8(10), Fig 5F. doi:10.1371/journal.pone.0076466 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• Schematic Diagram

Supplier Data

Schematic Diagram - Cathepsin B Activity Assay Kit (Fluorometric) (AB65300)

Representative image of Cathepsin B Activity Assay Kit (Fluorometric) ab65300

Components shown from left to right :

- Cathepsin B Substrate

- Cathepsin Inhibitor

- Cathepsin Cell Lysis Buffer

- Cathepsin B Reaction Buffer

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.