JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB185438

Cathepsin B Inhibitor Screening Kit (Fluorometric)

Be the first to review this product! Submit a review

|

(4 Publications)

Abcam's Cathepsin B Inhibitor Screening Kit (ab185438) utilizes the ability of Cathepsin B to cleave the synthetic AFC based peptide substrate to release AFC, which can be easily quantified using a fluorometer or fluorescence microplate reader.

View Alternative Names

CPSB, CTSB, Cathepsin B, APP secretase, Cathepsin B1, APPS

5 Images
Functional Studies - Cathepsin B Inhibitor Screening Kit (Fluorometric) (AB185438)
  • FuncS

Lab

Functional Studies - Cathepsin B Inhibitor Screening Kit (Fluorometric) (AB185438)

Inhibition of Cathepsin B activity by the Cathepsin B inhibitor CTSB control.

Inhibition of Cathepsin B activity by the CTSB control

Functional Studies - Cathepsin B Inhibitor Screening Kit (Fluorometric) (AB185438)
  • FuncS

Supplier Data

Functional Studies - Cathepsin B Inhibitor Screening Kit (Fluorometric) (AB185438)

Inhibition of Cathepsin B activity by the Cathepsin B Inhibitor [F-F-FMK].

Inhibition of Cathepsin B activity by Cathepsin B Inhibitor Screening Kit was performed following kit protocol. This is example data only.

Functional Studies - Cathepsin B Inhibitor Screening Kit (Fluorometric) (AB185438)
  • FuncS

Lab

Functional Studies - Cathepsin B Inhibitor Screening Kit (Fluorometric) (AB185438)

Inhibition of Cathepsin B activity by the Cathepsin B inhibitor Chymostatin

Functional Studies - Cathepsin B Inhibitor Screening Kit (Fluorometric) (AB185438)
  • FuncS

Lab

Functional Studies - Cathepsin B Inhibitor Screening Kit (Fluorometric) (AB185438)

Inhibition of Cathepsin B activity by the Cathepsin B inhibitor E-64

Functional Studies - Cathepsin B Inhibitor Screening Kit (Fluorometric) (AB185438)
  • FuncS

Lab

Functional Studies - Cathepsin B Inhibitor Screening Kit (Fluorometric) (AB185438)

Inhibition of Cathepsin B activity by the Cathepsin B inhibitor Antipain dihydrochloride

Key facts

Detection method

Fluorescent

Sample types

Inhibitor compounds

Assay type

Semi-quantitative

Assay Platform

Microplate reader

style
left-column

Product details

Abcam's Cathepsin B Inhibitor Screening Kit (ab185438) utilizes the ability of Cathepsin B to cleave the synthetic AFC based peptide substrate to release AFC, which can be easily quantified using a fluorometer or fluorescence microplate reader. In the presence of a Cathepsin B-specific inhibitor, the cleavage of the substrate is reduced/abolished resulting in decrease or total loss of the AFC fluorescence. This high-throughput adaptable assay kit is simple, sensitive, and rapid tool to screen the potential inhibitors of Cathepsin B.

Visit our FAQs page for tips and troubleshooting.

This product is manufactured by BioVision, an Abcam company and was previously called K147 Cathepsin B (CTSB) Inhibitor Screening Kit (Fluorometric). K147-100 is the same size as the 100 test size of ab185438.

Cathepsin B (CTSB, EC 3.4.22.1) is a lysosomal cysteine proteinase that is suggested to participate in intracellular degradation and turnover of proteins. It has also been implicated in tumor invasion and metastasis.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

style
left-column

What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab185438-100Test", "assetComponentDetails": [ { "size":"1 x 20 µL", "name":"Cathepsin Inhibitor", "number":"AB185438-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"Cathepsin B Assay Buffer", "number":"AB185438-CMP04", "productcode":"" }, { "size":"1 x 100 µL", "name":"DTT I", "number":"AB185438-CMP02", "productcode":"" }, { "size":"1 x 5 µL", "name":"Human Cathepsin B", "number":"AB185438-CMP05", "productcode":"" }, { "size":"1 x 0.2 mL", "name":"Cathepsin B Substrate", "number":"AB185438-CMP03", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin B also known as CTSB or cathepsin beta is a lysosomal cysteine protease belonging to the papain family. This enzyme possesses a molecular weight of approximately 25 to 30 kDa. You can find cathepsin B expressed in various tissues with high levels observed in the liver kidney and spleen. It plays a mechanical role in protein degradation within lysosomes operating optimally in acidic environments. The enzyme has active and inactive forms depending on structural modifications and environmental conditions.
Biological function summary

Cathepsin B participates as a proteolytic enzyme involved in protein turnover and processing. It does not function in isolation but often associates with various other proteases forming complexes essential for efficient substrate breakdown. Cathepsin B assists in the activation of other enzymes by cleaving propeptides therefore facilitating the initiation of cascade reactions. This role is critical in maintaining cellular homeostasis and modulating physiological processes including cell migration and immune response modulation.

Pathways

Cathepsin B significantly contributes to the lysosomal protein degradation pathway and the autophagy-lysosome pathway. It interacts with proteins such as B protein which are important for cellular recycling and energy homeostasis. Its activity supports the MHC class II antigen processing pathway playing an essential part in immune system functionality. Cathepsin B also exhibits interactions with Ctsb protein inhibitors which regulate its proteolytic activity within these pathways.

Cathepsin B has strong associations with cancer progression and neurological disorders such as Alzheimer's disease. Its overexpression and dysregulation are linked to tumor metastasis where it degrades extracellular matrix components promoting invasion. Ctsb protein interactions with corey mazo proteins influence tumor microenvironment and immune evasion. In Alzheimer's disease cathepsin B can influence amyloid beta peptide processing connected to neurodegenerative pathology progression. These roles highlight the potential for cathepsin B as a therapeutic target in addressing these conditions.
style
left-column

Product protocols

style
left-column

Target data

Thiol protease which is believed to participate in intracellular degradation and turnover of proteins (PubMed : 12220505). Cleaves matrix extracellular phosphoglycoprotein MEPE (PubMed : 12220505). Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen (By similarity). Has also been implicated in tumor invasion and metastasis (PubMed : 3972105).
See full target information CTSB
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 11:9821-9832 PubMed34815788

2021

CST6 protein and peptides inhibit breast cancer bone metastasis by suppressing CTSB activity and osteoclastogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxun Li,Yajun Liang,Cheng Lian,Fangli Peng,Yansen Xiao,Yunfei He,Chengxin Ma,Yuan Wang,Peiyuan Zhang,Yuhan Deng,Yunpeng Su,Cheng Luo,Xiangyin Kong,Qingcheng Yang,Tong Liu,Guohong Hu

Journal of medicinal chemistry 65:2905-2925 PubMed34242027

2021

Peptidomimetic α-Acyloxymethylketone Warheads with Six-Membered Lactam P1 Glutamine Mimic: SARS-CoV-2 3CL Protease Inhibition, Coronavirus Antiviral Activity, and Biological Stability.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Bai,Alexandr Belovodskiy,Mostofa Hena,Appan Srinivas Kandadai,Michael A Joyce,Holly A Saffran,Justin A Shields,Muhammad Bashir Khan,Elena Arutyunova,Jimmy Lu,Sardeev K Bajwa,Darren Hockman,Conrad Fischer,Tess Lamer,Wayne Vuong,Marco J van Belkum,Zhengxian Gu,Fusen Lin,Yanhua Du,Jia Xu,Mohammad Rahim,Howard S Young,John C Vederas,D Lorne Tyrrell,M Joanne Lemieux,James A Nieman

Experimental & molecular medicine 50:1-14 PubMed30185799

2018

Down-regulation of cathepsin S and matrix metalloproteinase-9 via Src, a non-receptor tyrosine kinase, suppresses triple-negative breast cancer growth and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Jaya Gautam,Suhrid Banskota,Hyunji Lee,Yu-Jeong Lee,Yong Hyun Jeon,Jung-Ae Kim,Byeong-Seon Jeong

Biological & pharmaceutical bulletin 40:1240-1246 PubMed28502922

2017

Design, Synthesis, and Structure-Activity Relationship Study of Epoxysuccinyl-Peptide Derivatives as Cathepsin B Inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoye Zhang,Xiaohong Yang,Hongqiang Wang,Song Li,Kun Guo,Dan Jiang,Junhai Xiao,Di Liang
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB197012

Cathepsin L Inhibitor Assay Kit (Fluorometric)

Functional Studies - Cathepsin L Inhibitor Assay Kit (Fluorometric) (AB197012)

  • 0
  • 0
Assay Kits

AB65300

Cathepsin B Activity Assay Kit (Fluorometric)

Functional Studies - Cathepsin B Activity Assay Kit (Fluorometric) (AB65300)

  • 0
  • 0
Assay Kits

AB65302

Cathepsin D Activity Assay Kit (Fluorometric)

Functional Studies - Cathepsin D Activity Assay Kit (Fluorometric) (AB65302)

  • 4
  • 3
Assay Kits

AB272205

Human Cathepsin B ELISA Kit

Recombinant|SimpleStep

ELISA - Human Cathepsin B ELISA Kit (AB272205)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com