Cathepsin S Activity Assay Kit (ab65307) is a fluorescence-based assay that uses the preferred cathepsin-S substrate sequence VVR labeled with AFC (amino-4-trifluoromethyl coumarin).
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- American journal of translational research 16:544-5562024Dysregulation of kidney proteases in the pathogenesis of hypertension following unilateral nephrectomy in juvenile mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRasha Aly,Yunus E Dogan,Niharika Bala,Carlos Lugo,Seena Darwish,Lawrence Shoemaker,Abdel A AlliPubMed 38463588
- Cellular and molecular gastroenterology and hepatology 14:841-8762022Elafin Reverses Intestinal Fibrosis by Inhibiting Cathepsin S-Mediated Protease-Activated Receptor 2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Xie,Lindsey Fontenot,Andrea Chupina Estrada,Becca Nelson,Jiani Wang,David Q Shih,Wendy Ho,S Anjani Mattai,Florian Rieder,Dane D Jensen,Nigel W Bunnett,Hon Wai KoonPubMed 35840034
- Aging and disease 12:786-8002021Genome-Wide Screen and Validation of Microglia Pro-Inflammatory Mediators in Stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianhua Wu,Zhuoze Wu,Aodi He,Tongmei Zhang,Ping Zhang,Jing Jin,Sisi Li,Gaigai Li,Xinyan Li,Shiqi Liang,Lei Pei,Rong Liu,Qing Tian,Ximiao He,Youming Lu,Zhouping Tang,Hao LiPubMed 34094642
- International journal of molecular sciences 22:2021Rab27a Contributes to Cathepsin S Secretion in Lacrimal Gland Acinar Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRunzhong Fu,Maria C Edman,Sarah F Hamm-AlvarezPubMed 33562815
- The Journal of endocrinology 248:167-1792020Circulating cathepsin S improves glycaemic control in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHamzeh Karimkhanloo,Stacey N Keenan,Emily W Sun,David A Wattchow,Damien J Keating,Magdalene K Montgomery,Matthew J WattPubMed 33289685
- American journal of human genetics 107:527-5382020Myeloperoxidase Modulates Inflammation in Generalized Pustular Psoriasis and Additional Rare Pustular Skin Diseases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStefan Haskamp,Heiko Bruns,Madelaine Hahn,Markus Hoffmann,Anne Gregor,Sabine Löhr,Jonas Hahn,Christine Schauer,Mark Ringer,Cindy Flamann,Benjamin Frey,Adam Lesner,Christian T Thiel,Arif B Ekici,Stephan von Hörsten,Gunter Aßmann,Claudia Riepe,Maximilien Euler,Knut Schäkel,Sandra Philipp,Jörg C Prinz,Rotraut Mößner,Florina Kersting,Michael Sticherling,Abdelaziz Sefiani,Jaber Lyahyai,Wiebke Sondermann,Vinzenz Oji,Peter Schulz,Dagmar Wilsmann-Theis,Heinrich Sticht,Georg Schett,André Reis,Steffen Uebe,Silke Frey,Ulrike HüffmeierPubMed 32758447
- Biochemistry 59:1420-14272020Rational Design of a Humanized Antibody Inhibitor of Cathepsin B.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhefu Dai,Qinqin Cheng,Yong ZhangPubMed 32212642
- Scientific reports 10:14552020Cathepsin S activation contributes to elevated CX3CL1 (fractalkine) levels in tears of a Sjögren's syndrome murine model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRunzhong Fu,Hao Guo,Srikanth Janga,Minchang Choi,Wannita Klinngam,Maria C Edman,Sarah F Hamm-AlvarezPubMed 31996771
- Molecular pharmaceutics 16:3024-30392019Berunda Polypeptides: Biheaded Rapamycin Carriers for Subcutaneous Treatment of Autoimmune Dry Eye Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChangrim Lee,Hao Guo,Wannita Klinngam,Srikanth R Janga,Frances Yarber,Santosh Peddi,Maria C Edman,Nishant Tiwari,Siyu Liu,Stan G Louie,Sarah F Hamm-Alvarez,J Andrew MacKayPubMed 31095909
- Phytomedicine : international journal of phytother 62:1529642019Phloretin suppresses metastasis by targeting protease and inhibits cancer stemness and angiogenesis in human cervical cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi-Hsuan Hsiao et. al.PubMed 31153059
Key facts
- Detection method
- Fluorescent
- Sample types
- Tissue Extracts, Cell Lysate
- Assay type
- Enzyme activity
- Assay time
- 2h
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Target data
Function
Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules and MHC class II antigen presentation (PubMed:30612035). The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L.
Alternative names
Cathepsin S, CTSS
What's included?
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Cathepsin S Activity Assay Kit (ab65307) is a fluorescence-based assay that uses the preferred cathepsin-S substrate sequence VVR labeled with AFC (amino-4-trifluoromethyl coumarin).
Key facts
- Detection method
- Fluorescent
- Sample types
- Tissue Extracts, Cell Lysate
- Assay type
- Enzyme activity
- Assay time
- 2h
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Cathepsin S Activity Assay Kit (ab65307) is a fluorescence-based assay that uses the preferred cathepsin-S substrate sequence VVR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin-S will cleave the synthetic substrate VVR-AFC to release free AFC. The released AFC can easily be quantified using a fluorometer or fluorescence plate reader.
This product is manufactured by BioVision, an Abcam company and was previously called K144 Cathepsin S Activity Fluorometric Assay Kit. K144-100 is the same size as the 100 test size of ab65307.
Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cathepsin S sometimes referred to as CTSS is a cysteine protease enzyme weighing about 24-29 kDa part of the papain family. It occurs mainly in lysosomes and is expressed in a variety of cells including antigen-presenting cells like macrophages dendritic cells and B lymphocytes. Cathepsin S functions to cleave proteins at lysines playing a central role in protein degradation and antigen processing. It distinguishes itself from other cathepsins by maintaining activity in an alkaline environment and is essential for the processing of invariant chain (Ii) in MHC class II molecules.
- Biological function summary
Cathepsin S participates in immune system modulation and matrix degradation. It stands out in its role in the immune response cleaving antigenic proteins for presentation by MHC class II therefore influencing T-cell activation. Although synonymous with lysosomal activity it also operates extracellularly contributing to tissue remodeling and inflammation. Cathepsin S is not part of a larger complex but interacts with MHC class II impacting antigen presentation.
- Pathways
This protease is critical in the immune system and extracellular matrix degradation pathways. In the context of the immune pathway CTSS interacts with other proteases such as Cathepsin L and Cathepsin B to perform antigen trimming and processing. Moreover CTSS supports the activation of T lymphocytes by ensuring the correct presentation of peptide fragments on MHC class II molecules. In matrix degradation CTSS indirectly influences collagen breakdown and interaction with other matrix metalloproteinases.
- Associated diseases and disorders
Cathepsin S is associated with autoimmune diseases like rheumatoid arthritis where it contributes to tissue inflammation and joint damage. It also relates to atherosclerosis promoting instability in atherosclerotic plaques which could result in heart disease. In rheumatoid arthritis interactions with cytokines like TNF-alpha amplify inflammatory responses while in atherosclerosis CTSS links with other proteases to degrade extracellular matrix components leading to vulnerable lesions.
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Functional Studies - Cathepsin S Activity Assay Kit (Fluorometric) (ab65307)
Cathepsin S Activity measured in tissue lysates (each with protein concentration of 4 mg/mL) after 1 hour of incubation.
Functional Studies - Cathepsin S Activity Assay Kit (Fluorometric) (ab65307)
Cathepsin S Activity measured in cell lysates (each with cells concentration of 4e7 cells/mL) after 1 hour of incubation with and without inhibitor (I).
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