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AB65307

Cathepsin S Activity Assay Kit (Fluorometric)

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(27 Publications)

Cathepsin S Activity Assay Kit (ab65307) is a fluorescence-based assay that uses the preferred cathepsin-S substrate sequence VVR labeled with AFC (amino-4-trifluoromethyl coumarin).

View Alternative Names

Cathepsin S, CTSS

3 Images
Functional Studies - Cathepsin S Activity Assay Kit (Fluorometric) (AB65307)
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Lab

Functional Studies - Cathepsin S Activity Assay Kit (Fluorometric) (AB65307)

Cathepsin S Activity measured in cell lysates (each with cells concentration of 4e7 cells/mL) after 1 hour of incubation with and without inhibitor (I).

Functional Studies - Cathepsin S Activity Assay Kit (Fluorometric) (AB65307)
  • FuncS

Lab

Functional Studies - Cathepsin S Activity Assay Kit (Fluorometric) (AB65307)

Cathepsin S Activity measured in tissue lysates (each with protein concentration of 4 mg/mL) after 1 hour of incubation.

Schematic Diagram - Cathepsin S Activity Assay Kit (Fluorometric) (AB65307)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Cathepsin S Activity Assay Kit (Fluorometric) (AB65307)

Representative image of Cathepsin S Activity Assay Kit (Fluorometric) ab65307

Components shown from left to right :

- Cathepsin S Substrate

- Cathepsin Inhibitor

- Cathepsin S Lysis Buffer

- Cathepsin S Assay Buffer

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Fluorescent

Sample types

Tissue Extracts, Cell Lysate

Assay type

Enzyme activity

Assay time

2h

Assay Platform

Microplate reader

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Complementary Products

Assay Kits

AB65300

Cathepsin B Activity Assay Kit (Fluorometric)

Functional Studies - Cathepsin B Activity Assay Kit (Fluorometric) (AB65300)

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Assay Kits

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Cathepsin D Activity Assay Kit (Fluorometric)

Functional Studies - Cathepsin D Activity Assay Kit (Fluorometric) (AB65302)

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Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

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Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

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Assay Kits

AB126780

Cathepsin G Activity Assay Kit (Colorimetric)

Functional Studies - Cathepsin G Activity Assay Kit (Colorimetric) (AB126780)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Cathepsin S Activity Assay Kit (ab65307) is a fluorescence-based assay that uses the preferred cathepsin-S substrate sequence VVR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin-S will cleave the synthetic substrate VVR-AFC to release free AFC. The released AFC can easily be quantified using a fluorometer or fluorescence plate reader.

Other Notes
This product was previously called K144 Biovision Cathepsin S Activity Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.

Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab65307-100Test", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"Cathepsin S Substrate", "number":"AB65307-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"Cathepsin S Lysis Buffer", "number":"AB65307-CMP01", "productcode":"" }, { "size":"1 x 20 µL", "name":"Cathepsin Inhibitor", "number":"AB65307-CMP02", "productcode":"" }, { "size":"1 x 5 mL", "name":"Cathepsin S Assay Buffer", "number":"AB65307-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin S sometimes referred to as CTSS is a cysteine protease enzyme weighing about 24-29 kDa part of the papain family. It occurs mainly in lysosomes and is expressed in a variety of cells including antigen-presenting cells like macrophages dendritic cells and B lymphocytes. Cathepsin S functions to cleave proteins at lysines playing a central role in protein degradation and antigen processing. It distinguishes itself from other cathepsins by maintaining activity in an alkaline environment and is essential for the processing of invariant chain (Ii) in MHC class II molecules.
Biological function summary

Cathepsin S participates in immune system modulation and matrix degradation. It stands out in its role in the immune response cleaving antigenic proteins for presentation by MHC class II therefore influencing T-cell activation. Although synonymous with lysosomal activity it also operates extracellularly contributing to tissue remodeling and inflammation. Cathepsin S is not part of a larger complex but interacts with MHC class II impacting antigen presentation.

Pathways

This protease is critical in the immune system and extracellular matrix degradation pathways. In the context of the immune pathway CTSS interacts with other proteases such as Cathepsin L and Cathepsin B to perform antigen trimming and processing. Moreover CTSS supports the activation of T lymphocytes by ensuring the correct presentation of peptide fragments on MHC class II molecules. In matrix degradation CTSS indirectly influences collagen breakdown and interaction with other matrix metalloproteinases.

Cathepsin S is associated with autoimmune diseases like rheumatoid arthritis where it contributes to tissue inflammation and joint damage. It also relates to atherosclerosis promoting instability in atherosclerotic plaques which could result in heart disease. In rheumatoid arthritis interactions with cytokines like TNF-alpha amplify inflammatory responses while in atherosclerosis CTSS links with other proteases to degrade extracellular matrix components leading to vulnerable lesions.
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Product protocols

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Target data

Thiol protease (PubMed : 30612035). Key protease responsible for the removal of the invariant chain from MHC class II molecules and MHC class II antigen presentation (PubMed : 30612035). The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L (PubMed : 30612035). Elicits itch by mediating cleavage and activation of MRGPRX1 (By similarity).
See full target information CTSS
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Publications (27)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 122:e2425309122 PubMed40100622

2025

ACOD1-mediated lysosomal membrane permeabilization contributes to -induced macrophage death.

Applications

Unspecified application

Species

Unspecified reactive species

Ziwei Yang,Li Zhang,Samantha Ottavi,Jacob B Geri,Andrew Perkowski,Xiuju Jiang,Daniel Pfau,Ruslana Bryk,Jeffrey Aubé,Matthew Zimmerman,Véronique Dartois,Carl Nathan

Viruses 17: PubMed39861814

2025

Activity of Various Cathepsin Proteases and Enrichment of Klotho Protein in the Urine and Urinary Extracellular Vesicles After SARS-CoV-2 Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Niharika Bala,Ramish H Rafay,Sarah C Glover,Abdel A Alli

Cancer science 115:2036-2048 PubMed38613358

2024

Radiosensitizing effect of a novel CTSS inhibitor by enhancing BRCA1 protein stability in triple-negative breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Eun Choi,Kyung-Hwa Jeon,Hanhee Lee,Gil-Im Mun,Jeong-Ahn Kim,Jae-Ho Shin,Youngjoo Kwon,Younghwa Na,Yun-Sil Lee

Experimental and therapeutic medicine 27:142 PubMed38476910

2024

Fluorofenidone attenuates renal fibrosis by inhibiting lysosomal cathepsin‑mediated NLRP3 inflammasome activation.

Applications

Unspecified application

Species

Unspecified reactive species

Linfeng Zheng,Wenjuan Mei,Jing Zhou,Xin Wei,Zhijuan Huang,Xiaozhen Lin,Li Zhang,Wei Liu,Qian Wu,Jinhong Li,Yan Yan

American journal of translational research 16:544-556 PubMed38463588

2024

Dysregulation of kidney proteases in the pathogenesis of hypertension following unilateral nephrectomy in juvenile mice.

Applications

Unspecified application

Species

Unspecified reactive species

Rasha Aly,Yunus E Dogan,Niharika Bala,Carlos Lugo,Seena Darwish,Lawrence Shoemaker,Abdel A Alli

International journal of molecular sciences 24: PubMed37569859

2023

Augmentation of Cathepsin Isoforms in Diabetic db/db Mouse Kidneys Is Associated with an Increase in Renal MARCKS Expression and Proteolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammed F Gholam,Niharika Bala,Yunus E Dogan,Abdel A Alli

Cellular and molecular gastroenterology and hepatology 14:841-876 PubMed35840034

2022

Elafin Reverses Intestinal Fibrosis by Inhibiting Cathepsin S-Mediated Protease-Activated Receptor 2.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Xie,Lindsey Fontenot,Andrea Chupina Estrada,Becca Nelson,Jiani Wang,David Q Shih,Wendy Ho,S Anjani Mattai,Florian Rieder,Dane D Jensen,Nigel W Bunnett,Hon Wai Koon

Aging and disease 12:786-800 PubMed34094642

2021

Genome-Wide Screen and Validation of Microglia Pro-Inflammatory Mediators in Stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Jianhua Wu,Zhuoze Wu,Aodi He,Tongmei Zhang,Ping Zhang,Jing Jin,Sisi Li,Gaigai Li,Xinyan Li,Shiqi Liang,Lei Pei,Rong Liu,Qing Tian,Ximiao He,Youming Lu,Zhouping Tang,Hao Li

International journal of molecular sciences 22: PubMed33562815

2021

Rab27a Contributes to Cathepsin S Secretion in Lacrimal Gland Acinar Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Runzhong Fu,Maria C Edman,Sarah F Hamm-Alvarez

The Journal of endocrinology 248:167-179 PubMed33289685

2020

Circulating cathepsin S improves glycaemic control in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hamzeh Karimkhanloo,Stacey N Keenan,Emily W Sun,David A Wattchow,Damien J Keating,Magdalene K Montgomery,Matthew J Watt
View all publications
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