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AB284540

CD38 (Cyclase) Activity Assay Kit (Fluorometric)

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CD38 (Cyclase) Activity Assay Kit (Fluorometric) ab284540 (previously known as K2042) can be used to detect its activity in biological samples.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

CD38, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, 2'-phospho-ADP-ribosyl cyclase, 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-cyclic-ADP-ribose transferase, ADP-ribosyl cyclase 1, Cyclic ADP-ribose hydrolase 1, T10, ADPRC 1, cADPR hydrolase 1

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Functional Studies - CD38 (Cyclase) Activity Assay Kit (Fluorometric) (AB284540)
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Supplier Data

Functional Studies - CD38 (Cyclase) Activity Assay Kit (Fluorometric) (AB284540)

CD38 (Cyclase) Activity Assay Kit (Fluorometric).

CD38 activity assayed in rat spleen lysate (2 μg) and B-cell lysate (2.7 μg). Assays were performed following the kit protocol.

Functional Studies - CD38 (Cyclase) Activity Assay Kit (Fluorometric) (AB284540)
  • FuncS

Supplier Data

Functional Studies - CD38 (Cyclase) Activity Assay Kit (Fluorometric) (AB284540)

CD38 (Cyclase) Activity Assay Kit (Fluorometric).

CD38 Standard Curve. Assays were performed following the kit protocol.

Functional Studies - CD38 (Cyclase) Activity Assay Kit (Fluorometric) (AB284540)
  • FuncS

Supplier Data

Functional Studies - CD38 (Cyclase) Activity Assay Kit (Fluorometric) (AB284540)

CD38 (Cyclase) Activity Assay Kit (Fluorometric).

Reaction Kinetic curve of CD38 Positive Control, spleen lysate and B-cell lysate. Assays were performed following the kit protocol.

Key facts

Detection method

Fluorescent

Sample types

Tissue, Suspension cells, Adherent cells

Assay type

Enzyme activity

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This CD38 (Cyclase) Activity Assay Kit (Fluorometric) ab284540 (previously known as K2042) can be used to detect its activity in biological samples. The kit utilizes the activity of CD38 (Cyclase) to catalyze the conversion of a CD38 substrate to a fluorescent product (Ex/Em = 300/410 nm).

This assay kit provides a rapid, simple and sensitive method to detect CD38 (Cyclase) activity in a variety of sample types.

General range: 0 - 200 pmol/well

This product is manufactured by BioVision, an Abcam company and was previously called K2042 CD38 (Cyclase) Activity Assay Kit (Fluorometric). K2042-100 is the same size as the 100 test size of ab284540.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab284540-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"CD38 Positive Control", "number":"AB284540-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"CD38 Lysis Buffer", "number":"AB284540-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"CD38 Assay Buffer", "number":"AB284540-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"CD38 (Cyclase) Substrate", "number":"AB284540-CMP05", "productcode":"" }, { "size":"1 x 20 µL", "name":"CD38 Standard", "number":"AB284540-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Synthesizes cyclic ADP-ribose (cADPR), a second messenger for glucose-induced insulin secretion (PubMed : 7961800, PubMed : 8253715). Synthesizes the Ca(2+) mobilizer nicotinate-adenine dinucleotide phosphate, NAADP(+), from 2'-phospho-cADPR and nicotinic acid, as well as from NADP(+) and nicotinic acid. At both pH 5.0 and pH 7.4 preferentially transforms 2'-phospho-cADPR into NAADP(+), while preferentially cleaving NADP(+) to cADPR and ADPRP rather than into NADDP(+) (PubMed : 16690024). Has cADPR hydrolase activity (PubMed : 7961800, PubMed : 8253715).
See full target information CD38
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