Cdc42 Activation Assay Kit

Product details

Cdc42 Activation Assay Kit (ab211163) uses PAK1 PBD Agarose beads to selectively isolate and pull-down the active form of Cdc42 from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Cdc42 is detected by western blot analysis using an anti-Cdc42 specific monoclonal antibody, which reacts with the human, mouse and rat protein.

Features: 1) non radioactive assay format; 2) fast results: 1 hour assay plus electrophoresis/blotting time; 3) includes Cdc42 positive control; 4) pink colored agarose beads for easy identification during washing and aspiration steps.

Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways. Cdc42, a 21 kDa protein, belongs to the family of Rho GTPases and regulates a variety of biological response pathways that include cell motility, cell division, gene transcription, and cell transformation. Like other small GTPases, Cdc42 regulates molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In its active (GTP-bound) state, Cdc42 binds specifically to the p21-binding domain (PBD) of p21-activated protein kinase (PAK) to control downstream signaling cascades.

Cdc42 is overexpressed in NSCLC (non-small cell lung cancer), colorectal adenocarcinoma, melanoma, breast cancer and testicular cancer.

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