Cell Counting Kit 8 ab228554 is a no-wash assay with one incubation of 1-4 hours. WST-8 / CCK8 tetrazolium in solution is very stable with low cytotoxicity, and is reduced by cellular dehydrogenases with colorimetric (460 nm) readout proportional to the number of living cells.
Images
Publications
- International journal of surgery (London, England) 110:3269-32842024An anti-infection and biodegradable TFRD-loaded porous scaffold promotes bone regeneration in segmental bone defects: experimental studies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaixiong Lin,Zige Li,Zhenze Xie,Shengyao Tang,Minling Huang,Junjie Feng,Yuhan Wei,Zhen Shen,Ruoyu Zhou,Yuanlan Feng,Huamei Chen,Yueyi Ren,Feng Huang,Xiaotong Wang,Ziwei JiangPubMed 38506734
- Experimental dermatology 33:e150062024Q-switched 1064 nm Nd: YAG laser restores skin photoageing by activating autophagy by TGFβ1 and ITGB1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuiyi Xiang,Xiaorong Jia,Xiaoxia Duan,Qi Xu,Ruiqi Zhang,Yunting He,Zhi YangPubMed 38284200
Key facts
- Detection method
Colorimetric
- Sample types
Suspension cells, Adherent cells
Abcam Recommends
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
What's included?
Recommended products
Cell Counting Kit 8 ab228554 is a no-wash assay with one incubation of 1-4 hours. WST-8 / CCK8 tetrazolium in solution is very stable with low cytotoxicity, and is reduced by cellular dehydrogenases with colorimetric (460 nm) readout proportional to the number of living cells.
Key facts
- Detection method
Colorimetric
- Sample types
Suspension cells, Adherent cells
- Assay Platform
Microplate reader
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Cell Counting Kit 8 (WST-8 / CCK8) (ab228554) provides a convenient and robust way of performing a cell viability assay. The kit uses a water-soluble tetrazolium salt to quantify the number of live cells by producing an orange formazan dye upon bio-reduction in the presence of an electron carrier.
WST-8 / CCK8 solution is added directly to the test cells with no pre-mixing of components required.
WST-8 / CCK8 tetrazolium salt is reduced by cellular dehydrogenases to an orange formazan product that is soluble in tissue culture medium. The amount of formazan produced is directly proportional to the number of living cells and is measured by absorbance at 460 nm.
The excellent stability and little cytotoxicity of the WST-8 / CCK8 solution make the kit useful for assays that require long incubation (such as 24 to 48 hours).
The detection sensitivity is higher than with other tetrazolium salt-based assays such as MTT, XTT or MTS etc.
Review the to learn about other kits to perform a , or .
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Functional Studies - Cell Counting Kit 8 (WST-8 / CCK8) (ab228554)
Cytotoxicity tests of HeLa cells in response to (A) SDS and (B) Staurosporine treatment were measured with The cell Counting Kit 8 (WST-8) (ab228554). HeLa cells at 10,000 cells/well/100 μL were seeded overnight in a black wall/clear bottom 96-well plate. Cells were treated with serially diluted SDS for 2 hours or Staurosporine for 4 hours. The absorbance was measured at 460 nm using a plate reader.
Functional Studies - Cell Counting Kit 8 (WST-8 / CCK8) (ab228554)
Cell Counting Assay.
Cell number was determined with the cell Counting Kit 8 (WST-8) (ab228554). (A) HeLa cells at 0 to 10,000 cells/well/100 μL, and (B) Jurkat cells at 0 to 100,000 cells/well/100 μL were added in a clear bottom 96-well plate. The absorbance was measured at 460 nm using a plate reader.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com