Cell Counting Kit 8 / CCK-8 Assay Kit / WST-8 Assay Kit ab228554 is a no-wash, mix-and-read, cell viability assay. The ready-to-use solution is added to cell cultures for a 1-4 hour incubation, with a colorimetric (460 nm) readout proportional to the number of living cells.
- CCK-8 / WST-8 tetrazolium is reduced by cellular dehydrogenases in living cells and has very low cytotoxicity
- The CCK-8 assay has higher sensitivity than other tetrazolium salt-based assays, such as MTT, MTS or XTT
- Highly cited in >200 publications
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- International journal of surgery (London, England) 110:3269-32842024An anti-infection and biodegradable TFRD-loaded porous scaffold promotes bone regeneration in segmental bone defects: experimental studies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaixiong Lin,Zige Li,Zhenze Xie,Shengyao Tang,Minling Huang,Junjie Feng,Yuhan Wei,Zhen Shen,Ruoyu Zhou,Yuanlan Feng,Huamei Chen,Yueyi Ren,Feng Huang,Xiaotong Wang,Ziwei JiangPubMed 38506734
- Experimental dermatology 33:e150062024Q-switched 1064 nm Nd: YAG laser restores skin photoageing by activating autophagy by TGFβ1 and ITGB1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuiyi Xiang,Xiaorong Jia,Xiaoxia Duan,Qi Xu,Ruiqi Zhang,Yunting He,Zhi YangPubMed 38284200
- Cellular and molecular biology (Noisy-le-Grand, France) 69:137-1422024The effect of thalidomide on the invasive ability of gastric cancer cells by regulating miR-524-5p/FSTL1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaowei Qin,Jinjie Zhang,Mengmeng Wang,Yafei Feng,Yanbo Zhao,Huipeng Zhang,Wei GuoPubMed 38279459
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 38:e234012024Ferrostatin-1 inhibits ferroptosis of vascular smooth muscle cells and alleviates abdominal aortic aneurysm formation through activating the SLC7A11/GPX4 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin He,Yunchuan Xiong,Yu Liu,Yaozhen Li,Haiyang Zhou,Kemin WuPubMed 38236196
- International journal of molecular sciences 25:2024A Novel Role for DOC2B in Ameliorating Palmitate-Induced Glucose Uptake Dysfunction in Skeletal Muscle Cells via a Mechanism Involving β-AR Agonism and Cofilin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinhee Hwang,Rekha Balakrishnan,Eunjin Oh,Rajakrishnan Veluthakal,Debbie C ThurmondPubMed 38203312
- Molecular neurobiology 61:5282-52942024Fluvoxamine Exerts Sigma-1R to Rescue Autophagy via Pom121-Mediated Nucleocytoplasmic Transport of TFEB.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChun-Yu Lin,Hsiang-En Wu,Eddie Feng-Ju Weng,Hsuan-Cheng Wu,Tsung-Ping Su,Shao-Ming WangPubMed 38180612
- Journal of experimental & clinical cancer research : CR 42:3462023Targeting of RRM2 suppresses DNA damage response and activates apoptosis in atypical teratoid rhabdoid tumor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLe Hien Giang,Kuo-Sheng Wu,Wei-Chung Lee,Shing-Shung Chu,Anh Duy Do,Chun A Changou,Huy Minh Tran,Tsung-Han Hsieh,Hsin-Hung Chen,Chia-Ling Hsieh,Shian-Ying Sung,Alice L Yu,Yun Yen,Tai-Tong Wong,Che-Chang ChangPubMed 38124207
- Aging 15:14172-141912023Gypenoside IX restores Akt/GSK-3β pathway and alleviates Alzheimer's disease-like neuropathology and cognitive deficits.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLing Lei,Yong Luo,Dongkun Kang,Fumin Yang,Dongli Meng,Jian-Zhi Wang,Rong Liu,Xiaochuan Wang,Hong-Lian LiPubMed 38095632
- Current issues in molecular biology 45:9868-98862023Hydroxycitric Acid Alleviated Lung Ischemia-Reperfusion Injury by Inhibiting Oxidative Stress and Ferroptosis through the Hif-1α Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZi-Long Lu,Cong-Kuan Song,Shi-Shi Zou,Shi-Ze Pan,Kai Lai,Ning Li,Qing GengPubMed 38132462
- Journal of immunology research 2023:89246032023Suppression of NK Cell Activation by JAK3 Inhibition: Implication in the Treatment of Autoimmune Diseases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWai Chung Wu,Carol Shiu,Tak Keung Tong,Shui On Leung,Chin Wai HuiPubMed 38106519
Key facts
- Detection method
- Colorimetric
- Sample types
- Suspension cells, Adherent cells
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Cell Counting Kit 8 / CCK-8 Assay Kit / WST-8 Assay Kit ab228554 is a no-wash, mix-and-read, cell viability assay. The ready-to-use solution is added to cell cultures for a 1-4 hour incubation, with a colorimetric (460 nm) readout proportional to the number of living cells.
- CCK-8 / WST-8 tetrazolium is reduced by cellular dehydrogenases in living cells and has very low cytotoxicity
- The CCK-8 assay has higher sensitivity than other tetrazolium salt-based assays, such as MTT, MTS or XTT
- Highly cited in >200 publications
Key facts
- Detection method
- Colorimetric
- Sample types
- Suspension cells, Adherent cells
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Cell Counting Kit 8 / CCK-8 Assay Kit / WST-8 Assay Kit ab228554 provides a convenient and robust way of performing a cell viability assay.
How the CCK-8 assay works
The CCK-8 assay uses a water-soluble tetrazolium salt to quantify the number of live cells by producing an orange formazan dye upon bio-reduction in the presence of an electron carrier. WST-8 solution is added directly to the test cells, no pre-mixing of components is required. WST-8 tetrazolium salt is reduced by cellular dehydrogenases to an orange formazan product that is soluble in tissue culture medium.
The amount of formazan produced is directly proportional to the number of living cells by monitoring absorbance increase at 460 nm. The excellent stability and little cytotoxicity of WST-8 solution make the kit useful for the assays that require long incubation (such as 24 to 48 hours).
CCK8 assay protocol summary
- - Prepare cells in a 96-well plate (100 µL/well).
- - Add 10 µL of WST-8 Solution to each well.
- - Incubate at 37°C for 1 - 4 hours.
- - Monitor absorbance at OD = 460 nm.
How other researchers are using CCK8 Kit ab228554
Cell Counting Kit 8 (WST-8 / CCK8) has been used in a variety of sample type including:
- - Human Umbilical Vein Endothelial Cells (HUVECs) and Bone marrow stromal cells (BMSCs)1
- - Human peripheral blood mononuclear cells (PBMCs)2
- - Esophageal carcinoma cell lines (ESCC)3
References: 1- Lin H et al. 2024; 2- Wu WC et al. 2023; 3- Wei D et al. 2023
Related and recommended products
See other alternative kits to assess cell viability and proliferation:
- - XTT Assay Kit XTT Assay Kit ab232856
- - MTS Assay Kit (Cell Proliferation) (Colorimetric) MTS Assay Kit (Cell Proliferation) (Colorimetric) ab197010
- - MTT Assay Kit (Cell Proliferation) MTT Assay Kit (Cell Proliferation) ab211091
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Functional Studies - Cell Counting Kit 8 (WST-8 / CCK8) (ab228554)
Cytotoxicity tests of HeLa cells in response to (A) SDS and (B) Staurosporine treatment were measured with The cell Counting Kit 8 (WST-8) (ab228554). HeLa cells at 10,000 cells/well/100 μL were seeded overnight in a black wall/clear bottom 96-well plate. Cells were treated with serially diluted SDS for 2 hours or Staurosporine for 4 hours. The absorbance was measured at 460 nm using a plate reader.
Functional Studies - Cell Counting Kit 8 (WST-8 / CCK8) (ab228554)
Cell Counting Assay.
Cell number was determined with the cell Counting Kit 8 (WST-8) (ab228554). (A) HeLa cells at 0 to 10,000 cells/well/100 μL, and (B) Jurkat cells at 0 to 100,000 cells/well/100 μL were added in a clear bottom 96-well plate. The absorbance was measured at 460 nm using a plate reader.
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