This Cell Cycle Analysis Kit ab287852 (previously known as EZCell Cell Cycle Analysis Kit, K920) provides a quick and easy method to detect the number of cells in a cell population, which are at a specific stage of the cell cycle.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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Key facts
- Detection method
Flow cytometry-fluorescent
- Sample types
Suspension cells, Adherent cells
- Assay type
Cell-based
- Reactive species
Mammals
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Flow Cyt | Reactivity Reacts | Dilution info - | Notes - |
What's included?
Recommended products
This Cell Cycle Analysis Kit ab287852 (previously known as EZCell Cell Cycle Analysis Kit, K920) provides a quick and easy method to detect the number of cells in a cell population, which are at a specific stage of the cell cycle.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
Flow cytometry-fluorescent
- Sample types
Suspension cells, Adherent cells
- Assay type
Cell-based
- Reactive species
Mammals
- Assay Platform
Flow cytometer
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
This Cell Cycle Analysis Kit ab287852 (previously known as EZCell Cell Cycle Analysis Kit, K920) provides a quick and easy method to detect the number of cells in a cell population, which are at a specific stage of the cell cycle. The kit utilizes a nuclear dye, the binding of which to nucleic acids in the cell results in fluorescence signal, which is proportional to cellular DNA content. The percentages of cells in different phases of the cell cycle (G0/G1, S, and G2/M) can be quantified by flow cytometry. The method is non-radioactive, rapid and accurate and can be used for high throughput cell cycle analysis with contemporary flow cytometer instruments.
Key features of this kit:
• Highly sensitive flow cytometry-based method to detect and monitor cells at various stages of the cell cycle
• Simple & High throughput-adaptable
• Easy tool for screening compounds that affect cell growth and division
This product is manufactured by BioVision, an Abcam company and was previously called K920 EZCellTM Cell Cycle Analysis Kit. K920-100 is the same size as the 100 test size of ab287852.
Product promise
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2 product images
Functional Studies - Cell Cycle Analysis Kit (ab287852)
Cell cycle analysis of 3T3 cells using ab287852 in 10% FBS culture medium: 3T3 cells were seeded at 2 x 105 cells/well into a 6-well tissue culture plate with 10% FBS culture medium on day 1. On day 2, medium was removed and cells synchronized with 0.1% FBS culture medium for 24 hr. On day 3, 3T3 cells were incubated in culture medium with either 0.1% FBS, 10% FBS, or 10% FBS containing 20 μM of SKPin C1 (G1 or G2/M phase blocker) for an additional 24 hr. On day 4, cells were harvested, fixed and stained with the Cell Cycle Analysis Kit according to the protocol. Fluorescence intensity was detected and recorded on a BD Flow Cytometer in FL-2 channel.
Functional Studies - Cell Cycle Analysis Kit (ab287852)
Cell cycle analysis of 3T3 cells using ab287852 in 10% FBS culture medium containing 20 μM of SKPin C1: 3T3 cells were seeded at 2 x 105 cells/well into a 6-well tissue culture plate with 10% FBS culture medium on day 1. On day 2, medium was removed and cells synchronized with 0.1% FBS culture medium for 24 hr. On day 3, 3T3 cells were incubated in culture medium with either 0.1% FBS, 10% FBS, or 10% FBS containing 20 μM of SKPin C1 (G1 or G2/M phase blocker) for an additional 24 hr. On day 4, cells were harvested, fixed and stained with the Cell Cycle Analysis Kit according to the protocol. Fluorescence intensity was detected and recorded on a BD Flow Cytometer in FL-2 channel.
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