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AB287852

Cell Cycle Analysis Kit

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(8 Publications)

Cell Cycle Analysis Kit ab287852 provides a quick and easy method to detect the number of cells in a cell population, which are at a specific stage of the cell cycle.

- Suitable for cell cycle analysis using flow cytometry
-Individual kit components are also available for purchase with a minimum order of 20 units.
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Functional Studies - Cell Cycle Analysis Kit (AB287852)
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Supplier Data

Functional Studies - Cell Cycle Analysis Kit (AB287852)

Cell cycle analysis of 3T3 cells using ab287852 in 10% FBS culture medium : 3T3 cells were seeded at 2 x 105 cells/well into a 6-well tissue culture plate with 10% FBS culture medium on day 1. On day 2, medium was removed and cells synchronized with 0.1% FBS culture medium for 24 hr. On day 3, 3T3 cells were incubated in culture medium with either 0.1% FBS, 10% FBS, or 10% FBS containing 20 μM of SKPin C1 (G1 or G2/M phase blocker) for an additional 24 hr. On day 4, cells were harvested, fixed and stained with the Cell Cycle Analysis Kit according to the protocol. Fluorescence intensity was detected and recorded on a BD Flow Cytometer in FL-2 channel.

Functional Studies - Cell Cycle Analysis Kit (AB287852)
  • FuncS

Supplier Data

Functional Studies - Cell Cycle Analysis Kit (AB287852)

Cell cycle analysis of 3T3 cells using ab287852 in 10% FBS culture medium containing 20 μM of SKPin C1 : 3T3 cells were seeded at 2 x 105 cells/well into a 6-well tissue culture plate with 10% FBS culture medium on day 1. On day 2, medium was removed and cells synchronized with 0.1% FBS culture medium for 24 hr. On day 3, 3T3 cells were incubated in culture medium with either 0.1% FBS, 10% FBS, or 10% FBS containing 20 μM of SKPin C1 (G1 or G2/M phase blocker) for an additional 24 hr. On day 4, cells were harvested, fixed and stained with the Cell Cycle Analysis Kit according to the protocol. Fluorescence intensity was detected and recorded on a BD Flow Cytometer in FL-2 channel.

Key facts

Detection method

Flow cytometry-fluorescent

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based

Assay Platform

Flow cytometer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This Cell Cycle Analysis Kit ab287852 (previously known as EZCell Cell Cycle Analysis Kit, K920) provides a quick and easy method to detect the number of cells in a cell population, which are at a specific stage of the cell cycle. The kit utilizes a nuclear dye, the binding of which to nucleic acids in the cell results in fluorescence signal, which is proportional to cellular DNA content. The percentages of cells in different phases of the cell cycle (G0/G1, S, and G2/M) can be quantified by flow cytometry. The method is non-radioactive, rapid and accurate and can be used for high throughput cell cycle analysis with contemporary flow cytometer instruments.

Key features of this kit:

• Highly sensitive flow cytometry-based method to detect and monitor cells at various stages of the cell cycle
• Simple & High throughput-adaptable
• Easy tool for screening compounds that affect cell growth and division

This product is manufactured by BioVision, an Abcam company and was previously called K920 EZCellTM Cell Cycle Analysis Kit. K920-100 is the same size as the 100 test size of ab287852.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab287852-100Test", "assetComponentDetails": [ { "size":"2 x 1 mL", "name":"Nuclear Dye I", "number":"AB287852-CMP03", "productcode":"" }, { "size":"2 x 250 µL", "name":"Enzyme A Solution", "number":"AB287852-CMP02", "productcode":"" }, { "size":"1 x 50 mL", "name":"10X Cell Cycle Assay Buffer", "number":"AB287852-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

PLoS computational biology 21:e1012847 PubMed40053523

2025

iSoMAs: Finding isoform expression and somatic mutation associations in human cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Tan,Valer Gotea,Sushil K Jaiswal,Nancy E Seidel,David O Holland,Kevin Fedkenheuer,Abdel G Elkahloun,Sara R Bang-Christensen,Laura Elnitski

BMC complementary medicine and therapies 25:59 PubMed39962423

2025

Cytotoxic and immunomodulatory properties of Tinospora cordifolia, Boerhaavia diffusa, Berberis aristata, and Ocimum basilicum extracts against HPV-positive cervical cancer cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Harsha Vaghasia,Rahul Patel,Jignesh Prajapati,Krupa Shah,Meenu Saraf,Rakesh M Rawal

Scientific reports 15:2123 PubMed39820554

2025

Loss of Sirtuin 7 impairs cell motility and proliferation and enhances S-phase cell arrest after 5-fluorouracil treatment in head and neck cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Halasa,Syeda Afshan,Anna Wawruszak,Agata Borkowska,Klaudia Brodaczewska,Alicja Przybyszewska-Podstawka,Joanna Kalafut,Marzena Baran,Adolfo Rivero-Müller,Andrzej Stepulak,Matthias Nees

Journal of medicinal chemistry 67:20100-20117 PubMed39530508

2024

Prioritization of Eleven-Nineteen-Leukemia Inhibitors as Orally Available Drug Candidates for Acute Myeloid Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Xuejiao Shirley Guo,Sandeep Atla,Satyanarayana Nyalata,Yugendar R Alugubelli,Peng-Hsun Chase Chen,Shiqing Xu,Wenshe Ray Liu

Nucleic acids research 52:13003-13018 PubMed39413208

2024

SNF2L suppresses nascent DNA gap formation to promote DNA synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Anthony Nelligan,Huzefa Dungrawala

Cancer research 84:3004-3022 PubMed38959335

2024

Asparagine Dependency Is a Targetable Metabolic Vulnerability in TP53-Altered Castration-Resistant Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Young A Yoo,Songhua Quan,William Yang,Qianyu Guo,Yara Rodríguez,Zachary R Chalmers,Mary F Dufficy,Barbara Lackie,Vinay Sagar,Kenji Unno,Mihai I Truica,Navdeep S Chandel,Sarki A Abdulkadir

PeerJ 12:e17356 PubMed38766485

2024

Hsa_circ_0009096/miR-370-3p modulates hepatic stellate cell proliferation and fibrosis during biliary atresia pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhouguang Wu,Bin Wang,Siqi Chen,Taoyan Zuo,Wenjie Zhang,Zhen Cheng,Jingru Fu,Jiafeng Gong

Scientific reports 14:3809 PubMed38360831

2024

Silver nanoparticle functionalized by glutamine and conjugated with thiosemicarbazide induces apoptosis in colon cancer cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Hadi Taati,Helia Sangani,Arash Davoudi,Samira Safabakhsh Kouchesfahani,Mohammad Hedayati,Sana Tarashandeh Hemmati,Taraneh Ghasemipour,Shahrzad Aghajani,Mahan Farah Andooz,Maryam Amanollahi,Fakhrieh Kalavari,Ali Salehzadeh
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com