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Cell Cycle Assay Kit (Fluorometric - Green) (ab112116) is designed to monitor cell cycle progression and proliferation by using our proprietary Nuclear Green CCS1 in live cells.

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Images

Flow Cytometry - Cell Cycle Assay Kit (Fluorometric - Green) (AB112116), expandable thumbnail
  • Flow Cytometry - Cell Cycle Assay Kit (Fluorometric - Green) (AB112116), expandable thumbnail

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Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Reactive species

Mammals

Associated Products

Select an associated product type

1 product for Alternative Product

What's included?

100 Test
Components
Assay Buffer
1 x 50 mL
Nuclear Green CCS1
1 x 250 µL

Recommended products

Cell Cycle Assay Kit (Fluorometric - Green) (ab112116) is designed to monitor cell cycle progression and proliferation by using our proprietary Nuclear Green CCS1 in live cells.

Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Reactive species

Mammals

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Cell Cycle Assay Kit (Fluorometric - Green) (ab112116) is designed to monitor cell cycle progression and proliferation by using our proprietary Nuclear Green CCS1 in live cells. The percentage of cells in a given sample that are in G0/G1, S and G2/M phases, as well as the cells in the sub-G1 phase prior to apoptosis can be determined by flow cytometry. Cells stained with Nuclear Green CCS1 can be monitored with a flow cytometer at Ex/Em = 490 nm/520 nm (FL1 channel). Visit our for tips and troubleshooting.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Flow Cytometry - Cell Cycle Assay Kit (Fluorometric - Green) (ab112116), expandable thumbnail

    Flow Cytometry - Cell Cycle Assay Kit (Fluorometric - Green) (ab112116)

    Histogram of HeLa cells (black: untreated cells, red : apoptotic cells) stained with 0.1x Nuclear Green CCS1

  • Flow Cytometry - Cell Cycle Assay Kit (Fluorometric - Green) (ab112116), expandable thumbnail

    Flow Cytometry - Cell Cycle Assay Kit (Fluorometric - Green) (ab112116)

    DNA profile in growing and camptothecin treated Jurkat cells.

    Jurkat cells were treated without (red) or with 20 µM camptothecin (blue) in a 37°C, 5% CO2 incubator for ~8 hours, then dye loaded with 200X Green Fluorescence Probe for 60 minutes. The fluorescence intensity of 200X Green Fluorescence Probe was measured with a flow cytometer using the FL1 channel.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com