ab112117 is designed to monitor cell cycle progression and proliferation by using our proprietary Nuclear Red CCS1 in fixed cells.
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- Journal of cellular physiology 235:4335-43502019miR-298 plays a pivotal role in colon cancer invasiveness by targeting PTEN.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZahra Arabsorkhi,Ehsan Gharib,Javad Yaghmoorian Khojini,Mohammad-Erfan Farhadieh,Ehsan Nazemalhosseini-Mojarad,Mohammad Reza ZaliPubMed 31621072
- Scientific reports 8:20982018Kinome and phosphoproteome of high-grade meningiomas reveal AKAP12 as a central regulator of aggressiveness and its possible role in progression.Applications:FuncSReactive species:Unspecified reactive speciesCarolina Angelica Parada,Joshua Osbun,Sumanpreet Kaur,Youssef Yakkioui,Min Shi,Catherine Pan,Tina Busald,Yigit Karasozen,Luis Francisco Gonzalez-Cuyar,Robert Rostomily,Jing Zhang,Manuel FerreiraPubMed 29391485
Key facts
- Detection method
- Fluorescent
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Cell-based (quantitative)
- Reactive species
- Mammals
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ab112117 is designed to monitor cell cycle progression and proliferation by using our proprietary Nuclear Red CCS1 in fixed cells.
Key facts
- Detection method
- Fluorescent
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Cell-based (quantitative)
- Reactive species
- Mammals
- Assay Platform
- Flow cytometer
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
ab112117 is designed to monitor cell cycle progression and proliferation by using our proprietary Nuclear Red CCS1 in fixed cells. The dye passes through a permeabilized membrane and intercalates into cellular DNA. The signal intensity of Nuclear Red CCS1 is directly proportional to DNA content after RNA is degraded by RNase provided in the kit. The percentage of cells in a given sample that are in G0/G1, S and G2/M phases, as well as the cells in the sub-G1 phase prior to apoptosis can be monitored with a flow cytometer at Ex/Em = 490/620 nm (FL2 channel).
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Store ab112117 desiccated.
Product promise
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Flow Cytometry - Cell Cycle Assay Kit (Fluorometric - Red) (ab112117)
DNA profile in growing Jurkat cells.
Jurkat cells were dye-loaded with Red Fluorescence Probe and RNase A for 30 minutes. The fluorescence intensity of Red Fluorescence Probe was measured with a flow cytometer, using the FL2 channel.
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