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AB112117

Cell Cycle Assay Kit (Fluorometric - Red)

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(4 Publications)

ab112117 is designed to monitor cell cycle progression and proliferation by using our proprietary Nuclear Red CCS1 in fixed cells.
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Flow Cytometry - Cell Cycle Assay Kit (Fluorometric - Red) (AB112117)
  • Flow Cyt

Supplier Data

Flow Cytometry - Cell Cycle Assay Kit (Fluorometric - Red) (AB112117)

DNA profile in growing Jurkat cells.
Jurkat cells were dye-loaded with Red Fluorescence Probe and RNase A for 30 minutes. The fluorescence intensity of Red Fluorescence Probe was measured with a flow cytometer, using the FL2 channel.

Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based (quantitative)

Assay Platform

Flow cytometer

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Product details

ab112117 is designed to monitor cell cycle progression and proliferation by using our proprietary Nuclear Red CCS1 in fixed cells. The dye passes through a permeabilized membrane and intercalates into cellular DNA. The signal intensity of Nuclear Red CCS1 is directly proportional to DNA content after RNA is degraded by RNase provided in the kit. The percentage of cells in a given sample that are in G0/G1, S and G2/M phases, as well as the cells in the sub-G1 phase prior to apoptosis can be monitored with a flow cytometer at Ex/Em = 490/620 nm (FL2 channel).

Visit our FAQs page for tips and troubleshooting.

Store ab112117 desiccated.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab112117-100Test", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"Assay Buffer", "number":"AB112117-CMP01", "productcode":"" }, { "size":"1 x 250 µL", "name":"100X RNase A", "number":"AB112117-CMP02", "productcode":"" }, { "size":"1 x 250 µL", "name":"Nuclear Red CCS1", "number":"AB112117-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadk6989 PubMed40203114

2025

Androgen receptor-regulated lncRNA PRCAT71 promotes AR signaling through the interaction with KHSRP in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yongyong Yang,Ting-You Wang,Qianru Li,Jiawen Lu,Yanan Ren,Adam B Weiner,Joshua Fry,Qi Liu,Chaehyun Yum,Rui Wang,Qingxiang Guo,Yu Wan,Zhe Ji,Xuesen Dong,Tamara L Lotan,Edward M Schaeffer,Rendong Yang,Qi Cao

Cell cycle (Georgetown, Tex.) 21:33-48 PubMed34974808

2022

Uridine relieves MSCs and chondrocyte senescence and exhibits the potential to treat osteoarthritis .

Applications

Unspecified application

Species

Unspecified reactive species

Jia Ye,Zhihui Jin,Sen Chen,Weichun Guo

Journal of cellular physiology 235:4335-4350 PubMed31621072

2019

miR-298 plays a pivotal role in colon cancer invasiveness by targeting PTEN.

Applications

Unspecified application

Species

Unspecified reactive species

Zahra Arabsorkhi,Ehsan Gharib,Javad Yaghmoorian Khojini,Mohammad-Erfan Farhadieh,Ehsan Nazemalhosseini-Mojarad,Mohammad Reza Zali

Scientific reports 8:2098 PubMed29391485

2018

Kinome and phosphoproteome of high-grade meningiomas reveal AKAP12 as a central regulator of aggressiveness and its possible role in progression.

Applications

FuncS

Species

Unspecified reactive species

Carolina Angelica Parada,Joshua Osbun,Sumanpreet Kaur,Youssef Yakkioui,Min Shi,Catherine Pan,Tina Busald,Yigit Karasozen,Luis Francisco Gonzalez-Cuyar,Robert Rostomily,Jing Zhang,Manuel Ferreira
View all publications
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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