Monitoring cell cytotoxicity is one of the most essential tasks for studying cellular functions.
Images
Publications
Filter by
- ACS omega 8:22698-227072023Rhodamine B-Conjugated Fluorescent Block Copolymer Micelles for Efficient Chlorambucil Delivery and Intracellular Imaging.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBhagyashree Kulkarni,Somayah Qutub,Niveen M Khashab,Nikos HadjichristidisPubMed 37396240
- Pharmaceutics 15:2023Hydroxychloroquine Enhances Cytotoxic Properties of Extracellular Vesicles and Extracellular Vesicle-Mimetic Nanovesicles Loaded with Chemotherapeutics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSergey Brezgin,Anastasiya Kostyusheva,Natalia Ponomareva,Ekaterina Bayurova,Alla Kondrashova,Anastasia Frolova,Olga Slatinskaya,Landysh Fatkhutdinova,Georgy Maksimov,Mikhail Zyuzin,Ilya Gordeychuk,Alexander Lukashev,Sergey Makarov,Alexander Ivanov,Andrey A Zamyatnin,Vladimir Chulanov,Alessandro Parodi,Dmitry KostyushevPubMed 36839856
- Cellular immunology 376:1045352022Gram-positive Staphylococcus aureus LTA promotes distinct memory-like effects in murine bone marrow neutrophils.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTrim Lajqi,David Frommhold,Maylis Braun,Simon Alexander Kranig,Johannes Pöschl,Hannes HudallaPubMed 35537323
- Nature communications 13:18952022Modification of BRCA1-associated breast cancer risk by HMMR overexpression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrancesca Mateo,Zhengcheng He,Lin Mei,Gorka Ruiz de Garibay,Carmen Herranz,Nadia García,Amanda Lorentzian,Alexandra Baiges,Eline Blommaert,Antonio Gómez,Oriol Mirallas,Anna Garrido-Utrilla,Luis Palomero,Roderic Espín,Ana I Extremera,M Teresa Soler-Monsó,Anna Petit,Rong Li,Joan Brunet,Ke Chen,Susanna Tan,Connie J Eaves,Curtis McCloskey,Razq Hakem,Rama Khokha,Philipp F Lange,Conxi Lázaro,Christopher A Maxwell,Miquel Angel PujanaPubMed 35393420
- Biomedicines 10:2022Advantages of Fibrin Polymerization Method without the Use of Exogenous Thrombin for Vascular Tissue Engineering Applications.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVera G Matveeva,Evgenia A Senokosova,Viktoriia V Sevostianova,Mariam Yu Khanova,Tatiana V Glushkova,Tatiana N Akentieva,Larisa V Antonova,Leonid S BarbarashPubMed 35453539
- Animal reproduction 19:e202100582022Effect of glycerol addition time on the cryopreserved Korean native brindle cattle (Chikso) sperm quality.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Ma,Dae-Hyun Kim,Eun-Ju Jung,Woo-Jin Lee,Ju-Mi Hwang,Jeong-Won Bae,Dae-Jin Jung,Jun Koo Yi,Sang Moo Lee,Jae Jung Ha,Woo-Sung KwonPubMed 35432606
- Neuro-oncology 24:1101-11122022Deuterium magnetic resonance spectroscopy enables noninvasive metabolic imaging of tumor burden and response to therapy in low-grade gliomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCéline Taglang,Georgios Batsios,Joydeep Mukherjee,Meryssa Tran,Anne Marie Gillespie,Donghyun Hong,Sabrina M Ronen,Hema Artee Luchman,Russell O Pieper,Pavithra ViswanathPubMed 35091751
- Pharmaceutics 14:2022Safety, Stability, and Therapeutic Efficacy of Long-Circulating TQ-Incorporated Liposomes: Implication in the Treatment of Lung Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArif Khan,Mohammed A Alsahli,Mohammad A Aljasir,Hamzah Maswadeh,Mugahid A Mobark,Faizul Azam,Khaled S Allemailem,Faris Alrumaihi,Fahad A Alhumaydhi,Ameen S S Alwashmi,Ahmed A Almatroudi,Mahdi H Alsugoor,Masood A KhanPubMed 35057049
- International journal of molecular sciences 22:2021LPS Induces Opposing Memory-like Inflammatory Responses in Mouse Bone Marrow Neutrophils.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTrim Lajqi,Maylis Braun,Simon Alexander Kranig,David Frommhold,Johannes Pöschl,Hannes HudallaPubMed 34575963
- Journal of dairy science 104:9450-94642021Biological activities of the bioaccessible compounds after in vitro digestion of low-fat Akawi cheese made from blends of bovine and camel milk.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMutamed Ayyash,Abdelmoneim Abdalla,Mouza Alameri,Mohd Affan Baig,Jaleel Kizhakkayil,Gang Chen,Thom Huppertz,Afaf Kamal-EldinPubMed 34147215
Key facts
- Detection method
- Colorimetric
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Quantitative
- Reactive species
- Mammals
What's included?
Recommended products
Monitoring cell cytotoxicity is one of the most essential tasks for studying cellular functions.
Key facts
- Detection method
- Colorimetric
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Quantitative
- Reactive species
- Mammals
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Monitoring cell cytotoxicity is one of the most essential tasks for studying cellular functions. There are a variety of parameters that can be used. ab112118 uses a proprietary water-soluble dye that changes its absorption spectra upon cellular reduction. The absorption ratio change is directly proportional to the number of living cells. The characteristics of its high sensitivity, non-radioactivity and no-wash method make ab112118 suitable for high throughput screening of cell proliferation or cytotoxicity against a variety of compounds.
ab112118 does not require pre-mixing of components and has higher sensitivity compared to tetrazolium based colorimetric assays (such as MTT and XTT). It comes with reagents sufficient to run 1000 assays. The kit components are quite stable with minimal cytotoxicity, thus longer incubation times (such as 24-48 hours) are possible if required.
ab112118 is robust and convenient to use. It can be readily adapted for a wide variety of instrument platforms. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format.
Visit our FAQs page for tips and troubleshooting.
Cytotoxicity assay protocol summary:
- add assay solution to cells and shake for 30 s
- incubate for 1-24 hrs
- analyze with microplate reader
Explore our curated range of easy-to-use, efficient and fast cell viability assays.
ab112118 should be stored desiccated.
As low as 300 cells can be accurately quantified using ab112118.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Functional Studies - Cell Cytotoxicity Assay Kit (Colorimetric) (ab112118)
•HeLa: day before experiment cells seeded in 96 wp @ 1e4 cells/well and treated with 1μM, 10 μM and 50 μM Camptothecin (incubated for 24h); on the day of experiment cells incubated with Assay Solution for 2 hours.
•NIH3T3: day before experiment cells seeded in 96 wp @ 1e4 cells/well and treated with 1μM, 10 μM and 50 μM Camptothecin (incubated for 24h); on the day of experiment cells incubated with Assay Solution for 2 hours.
Functional Studies - Cell Cytotoxicity Assay Kit (Colorimetric) (ab112118)
CHO-K1 cell number response was measured with ab112118. CHO-K1 cells at 0 to 10,000 cells/well/100 µL were seeded overnight in a black wall/clear bottom 96-well plate. The cells were incubated with 20 µL/well of Assay Solution for 3 hours at 37 oC. The absorbance intensity was measured at 570 nm and 605 nm. The ratio of OD570/OD605 is proportional to the number of cells as indicated.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com