Cell Invasion Assay (Basement Membrane), 96-well, 8 µm (ab235697) uses a Boyden chamber coated with Basement Membrane Extract (BME), where the cells invade the matrix and then migrate through a semi-permeable membrane in the Boyden chamber in response to stimulants or inhibitory compounds.
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- Cell death & disease 14:3192023Interferon-gamma is quintessential for NOS2 and COX2 expression in ER breast tumors that lead to poor outcome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRobert Y S Cheng,Lisa A Ridnour,Adelaide L Wink,Ana L Gonzalez,Elise L Femino,Helene Rittscher,Veena Somasundaram,William F Heinz,Leandro Coutinho,M Cristina Rangel,Elijah F Edmondson,Donna Butcher,Robert J Kinders,Xiaoxian Li,Stephen T C Wong,Daniel W McVicar,Stephen K Anderson,Milind Pore,Stephen M Hewitt,Timothy R Billiar,Sharon A Glynn,Jenny C Chang,Stephen J Lockett,Stefan Ambs,David A WinkPubMed 37169743
- Histology and histopathology 38:1321-13262023LncRNA RUNX1-IT1 is downregulated in gastric cancer and suppresses the maturation of miR-20a by binding to its precursor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Bao,Boxiang Du,Yunhu Guo,Huaguo Zhang,Zhongqi MaoPubMed 36722424
- BMC molecular and cell biology 23:262022Effects of the media conditioned by various macrophage subtypes derived from THP-1 cells on tunneling nanotube formation in pancreatic cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChia-Wei Lee,Chia-Chen Kuo,Chi-Jung Liang,Huei-Jyuan Pan,Chia-Ning Shen,Chau-Hwang LeePubMed 35794526
- Heliyon 8:e098812022Hepatitis B virus X protein and hepatitis C virus core protein cooperate to repress E-cadherin expression via DNA methylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyunyoung Yoon,Kyung Lib JangPubMed 35832344
- Frontiers in oncology 12:9183402022Vitamin D Exerts Significant Antitumor Effects by Suppressing Vasculogenic Mimicry in Breast Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKhuloud Bajbouj,Abeer Al-Ali,Jasmin Shafarin,Lina Sahnoon,Ahmad Sawan,Ahmed Shehada,Walaaeldin Elkhalifa,Maha Saber-Ayad,Jibran Sualeh Muhammad,Adel B Elmoselhi,Salman Y Guraya,Mawieh HamadPubMed 35747793
- International journal of oncology 60:2021Downregulation of METTL6 mitigates cell progression, migration, invasion and adhesion in hepatocellular carcinoma by inhibiting cell adhesion molecules.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmina Bolatkan,Ken Asada,Syuzo Kaneko,Kruthi Suvarna,Noriko Ikawa,Hidenori Machino,Masaaki Komatsu,Shuichiro Shiina,Ryuji HamamotoPubMed 34913069
- BMC pharmacology & toxicology 22:622021Famotidine promotes inflammation by triggering cell pyroptosis in gastric cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJin Huang,Pingsheng Fan,Miao Liu,Chengtao Weng,Gaofei Fan,Tengyue Zhang,Xiaohong Duan,Yang Wu,Lili Tang,Guohong Yang,Yabei LiuPubMed 34686215
- BMC pharmacology & toxicology 22:442021Rabeprazole inhibits inflammatory reaction by inhibition of cell pyroptosis in gastric epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Xie,Long Fan,Liya Xiong,Peiyu Chen,Hongli Wang,Huan Chen,Junhong Zhao,Zhaohui Xu,Lanlan Geng,Wanfu Xu,Sitang GongPubMed 34266494
- Journal of Cancer 12:404-4162021USP43 directly regulates ZEB1 protein, mediating proliferation and metastasis of colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDao-Xiong Ye,Si-Si Wang,Ying Huang,Xiao-Jie Wang,Pan ChiPubMed 33391437
- Cancer cell international 20:3462020USP18 directly regulates Snail1 protein through ubiquitination pathway in colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFakun Huang,Chengying Zheng,Longkai Huang,Changqing Lin,Jiaxing WangPubMed 32742193
Key facts
- Detection method
Fluorescent
- Sample types
Suspension cells, Adherent cells
- Reactive species
Mouse, Human
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Cell Invasion Assay (Basement Membrane), 96-well, 8 µm (ab235697) uses a Boyden chamber coated with Basement Membrane Extract (BME), where the cells invade the matrix and then migrate through a semi-permeable membrane in the Boyden chamber in response to stimulants or inhibitory compounds.
Key facts
- Detection method
Fluorescent
- Sample types
Suspension cells, Adherent cells
- Reactive species
Mouse, Human
- Assay Platform
Microplate reader
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Cell Invasion Assay (Basement Membrane), 96-well, 8 μm (ab235697) utilizes a Boyden chamber coated with Basement Membrane Extract (BME), where the cells invade the matrix and then migrate through a semi-permeable membrane in the Boyden chamber in response to stimulants or inhibitory compounds. The percent cell invasion can be analyzed directly in a plate reader. Our assay is easy to use, sensitive and adaptable to high-throughput systems.
This product is manufactured by BioVision, an Abcam company and was previously called K912 EZCellTM Cell Invasion Assay (Basement Membrane), 96-well, 8 μm. K912-100 is the same size as the 100 test size of ab235697.
Cell invasion is the ability of cells to migrate from one area to another through an extracellular matrix. Cell invasion is exhibited by both normal cells as well as cancerous cells in response to specific external signals, including chemical & mechanical stimuli. During invasion, extracellular matrix is enzymatically degraded by cellular proteases before cells migrate to the new location. Cell invasion is required for normal processes such as wound repair, vasculature formation and the inflammatory response as well as the abnormal invasion of tissues by tumor cells during metastasis.
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2 product images
Functional Studies - Cell Invasion Assay (Basement Membrane), 96-well, 8 µm (ab235697)
Standard Curve.
HT-1080 cells were harvested, counted and serially diluted to obtain desired cell number. Cells were incubated according to the protocol with Cell Invasion Dye and fluorescence (Em/Ex 530/590) was measured.
Functional Studies - Cell Invasion Assay (Basement Membrane), 96-well, 8 µm (ab235697)
Cell Invasion.
3T3-NIH and HT-1080 cells were starved overnight and treated with Control Invasion Inducer or remain untreated (No Treatment). Treatment with Control Invasion Inducer demonstrated a significant increase in invasion of HT 1080 cells as compared to 3T3-NIH control cells.
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