Cell Invasion Assay (Collagen I) ab235887 uses a Boyden chamber coated with collagen I; cells invade the matrix and then migrate through a semipermeable membrane in the Boyden chamber in response to a treatment. The percent cell invasion is measured with a plate reader.
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Key facts
- Detection method
Fluorescent
- Sample types
Suspension cells, Adherent cells
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Cell Invasion Assay (Collagen I) ab235887 uses a Boyden chamber coated with collagen I; cells invade the matrix and then migrate through a semipermeable membrane in the Boyden chamber in response to a treatment. The percent cell invasion is measured with a plate reader.
Key facts
- Detection method
Fluorescent
- Sample types
Suspension cells, Adherent cells
- Assay Platform
Microplate reader
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
This kit provides enough materials for 12 assays, i.e. to run 12 wells of assay, although it comes in a 24 well plate format.
Cell Invasion Assay (Collagen I), 24-well, 8 μm (ab235887) utilizes a Boyden chamber coated with collagen I, where the cells invade the matrix and then migrate through a semipermeable membrane in the Boyden chamber in response to stimulants or inhibitory compounds. The percent cell invasion can be analyzed directly in a plate reader. Our assay is easy to use, sensitive and adaptable to high-throughput systems.
This product is manufactured by BioVision, an Abcam company and was previously called K917 EZCellTM Cell Invasion Assay Kit (Collagen I), 12-well, 8 μm. K917-12 is the same size as the 12 test size of ab235887.
Cell invasion is the ability of cells to migrate from one area to another through an extracellular matrix. Cell invasion is exhibited by both normal cells as well as cancerous cells in response to specific external signals, including chemical & mechanical stimuli. During invasion, extracellular matrix is enzymatically degraded by cellular proteases before cells migrate to the new location. Cell invasion is required for normal processes such as wound repair, vasculature formation and the inflammatory response as well as the abnormal invasion of tissues by tumor cells during metastasis.
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Functional Studies - Cell Invasion Assay Kit (Collagen I), 24-well, 8 µm (ab235887)
HT-1080 cells were harvested, counted and serially diluted to obtain desired cell number. Cells were incubated according to the protocol with Cell Invasion Dye and fluorescence (Em/Ex 485/530) was measured.
Functional Studies - Cell Invasion Assay Kit (Collagen I), 24-well, 8 µm (ab235887)
Cell Invasion.
3T3-NIH and HT-1080 cells were starved overnight and treated with Control Invasion Inducer or remain untreated (No Treatment). Treatment with Control Invasion Inducer demonstrated a significant increase in invasion of HT 1080 cells as compared to 3T3-NIH control cells.
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