Cell Migration/Chemotaxis Assay Kit (24-well, 5 µm) (ab235696) uses a Boyden chamber, where the cells migrate through a semi-permeable membrane under different stimuli.
Fluorescent
Adherent cells, Suspension cells
Mouse, Human
Select an associated product type
Cell Migration/Chemotaxis Assay Kit (24-well, 5 µm) (ab235696) uses a Boyden chamber, where the cells migrate through a semi-permeable membrane under different stimuli.
Fluorescent
Adherent cells, Suspension cells
Mouse, Human
Microplate reader
Blue Ice
-20°C
-20°C
-20°C
Cell Migration/Chemotaxis Assay Kit (24-well, 5 μm) (ab235696) utilizes a Boyden chamber, where the cells migrate through a semi-permeable membrane under different stimuli. Cell migration can be analyzed directly by reading fluorescence (Ex/Em = 530/590 nm) in a plate reader. Our assay is easy to use, sensitive and adaptable to high-throughput systems.
This product is manufactured by BioVision, an Abcam company and was previously called K910 EZCell™ Cell Migration/Chemotaxis Assay Kit (24-well, 5 μm). K910-12 is the same size as the 12 test size of ab235696.
Cell invasion is the ability of cells to migrate from one area to another through an extracellular matrix. Cell invasion is exhibited by both normal cells as well as cancerous cells in response to specific external signals, including chemical and mechanical stimuli. During invasion, extracellular matrix is enzymatically degraded by cellular proteases before cells migrate to the new location. Cell invasion is required for normal processes such as wound repair, vasculature formation and the inflammatory response as well as the abnormal invasion of tissues by tumor cells during metastasis.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Standard Curve.
Monocytes/macrophage cells were harvested, counted and serially diluted to obtain desired cell number. Cells were incubated according to the protocol.
Cell Invasion.
Monocytes/macrophage cells were starved overnight and treated with Control (Cnt) Invasion Inducer for 24 or 48 hours or left untreated. Treatment with Control Invasion Inducer demonstrated a significant increase in invasion with time. Control reading was subtracted from inducer reading.
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