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Cell Migration/Chemotaxis Assay Kit (24-well, 5 µm) (ab235696) uses a Boyden chamber, where the cells migrate through a semi-permeable membrane under different stimuli.

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Functional Studies - Cell Migration/Chemotaxis Assay Kit (24-well, 5 µm) (AB235696), expandable thumbnail
  • Functional Studies - Cell Migration/Chemotaxis Assay Kit (24-well, 5 µm) (AB235696), expandable thumbnail

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Key facts

Detection method

Fluorescent

Sample types

Adherent cells, Suspension cells

Reactive species

Mouse, Human

Associated Products

Select an associated product type

4 products for Alternative Product

What's included?

12 Test
Components
Cell Dissociation Solution II
1 x 6 mL
Cell Dye I
1 x 1.5 mL
Cell Migration Chamber (12 x 5µm)
1 x 1 Unit
Control Migration Inducer
1 x 300 µL
Wash Buffer I
1 x 25 mL

Recommended products

Cell Migration/Chemotaxis Assay Kit (24-well, 5 µm) (ab235696) uses a Boyden chamber, where the cells migrate through a semi-permeable membrane under different stimuli.

Key facts

Detection method

Fluorescent

Sample types

Adherent cells, Suspension cells

Reactive species

Mouse, Human

Assay Platform

Microplate reader

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Cell Migration/Chemotaxis Assay Kit (24-well, 5 μm) (ab235696) utilizes a Boyden chamber, where the cells migrate through a semi-permeable membrane under different stimuli. Cell migration can be analyzed directly by reading fluorescence (Ex/Em = 530/590 nm) in a plate reader. Our assay is easy to use, sensitive and adaptable to high-throughput systems.

This product is manufactured by BioVision, an Abcam company and was previously called K910 EZCell™ Cell Migration/Chemotaxis Assay Kit (24-well, 5 μm). K910-12 is the same size as the 12 test size of ab235696.

Cell invasion is the ability of cells to migrate from one area to another through an extracellular matrix. Cell invasion is exhibited by both normal cells as well as cancerous cells in response to specific external signals, including chemical and mechanical stimuli. During invasion, extracellular matrix is enzymatically degraded by cellular proteases before cells migrate to the new location. Cell invasion is required for normal processes such as wound repair, vasculature formation and the inflammatory response as well as the abnormal invasion of tissues by tumor cells during metastasis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Functional Studies - Cell Migration/Chemotaxis Assay Kit (24-well, 5 µm) (ab235696), expandable thumbnail

    Functional Studies - Cell Migration/Chemotaxis Assay Kit (24-well, 5 µm) (ab235696)

    Standard Curve.

    Monocytes/macrophage cells were harvested, counted and serially diluted to obtain desired cell number. Cells were incubated according to the protocol.

  • Functional Studies - Cell Migration/Chemotaxis Assay Kit (24-well, 5 µm) (ab235696), expandable thumbnail

    Functional Studies - Cell Migration/Chemotaxis Assay Kit (24-well, 5 µm) (ab235696)

    Cell Invasion.

    Monocytes/macrophage cells were starved overnight and treated with Control (Cnt) Invasion Inducer for 24 or 48 hours or left untreated. Treatment with Control Invasion Inducer demonstrated a significant increase in invasion with time. Control reading was subtracted from inducer reading.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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