Cell Migration/Chemotaxis Assay Kit (96-well, 5 µm) (ab235693) is a trans-well migration assay using a Boyden chamber, where cells migrate through a semi-permeable membrane under different stimuli.
- Ready-to-use buffers, dissociation solution, migration inducer, and cell dye
- Includes Boyden cell migration chamber
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- International journal of molecular sciences 23:2022GSK343, an Inhibitor of Enhancer of Zeste Homolog 2, Reduces Glioblastoma Progression through Inflammatory Process Modulation: Focus on Canonical and Non-Canonical NF-κB/IκBα Pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah Adriana Scuderi,Alessia Filippone,Rossella Basilotta,Deborah Mannino,Giovanna Casili,Anna Paola Capra,Giulia Chisari,Lorenzo Colarossi,Serena Sava,Michela Campolo,Emanuela Esposito,Irene PaternitiPubMed 36430394
- Cell reports 35:1093032021Toll-like receptor 2 induces pathogenicity in Th17 cells and reveals a role for IPCEF in regulating Th17 cell migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKathryne E Marks,Stephanie Flaherty,Kristen M Patterson,Matthew Stratton,Gustavo J Martinez,Joseph M ReynoldsPubMed 34192530
- Cancer immunology research 9:200-2132020Targeted Deletion of CXCR2 in Myeloid Cells Alters the Tumor Immune Environment to Improve Antitumor Immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinming Yang,Chi Yan,Anna E Vilgelm,Sheau-Chiann Chen,Gregory D Ayers,Christopher A Johnson,Ann RichmondPubMed 33177110
Key facts
- Detection method
- Fluorescent
- Sample types
- Adherent cells, Suspension cells
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Cell Migration/Chemotaxis Assay Kit (96-well, 5 µm) (ab235693) is a trans-well migration assay using a Boyden chamber, where cells migrate through a semi-permeable membrane under different stimuli.
- Ready-to-use buffers, dissociation solution, migration inducer, and cell dye
- Includes Boyden cell migration chamber
Key facts
- Detection method
- Fluorescent
- Sample types
- Adherent cells, Suspension cells
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Cell Migration/Chemotaxis Assay Kit (96-well, 5 μm) (ab235693) utilizes a Boyden chamber, where the cells migrate through a semi-permeable membrane under different stimuli. Cell migration can be analyzed directly by reading fluorescence (Ex/Em = 530/590 nm) in a plate reader. The assay is easy to use, sensitive and adaptable to high-throughput systems.
Cell migration / chemotaxis assay protocol summary
- Grow cells.
- Add cell culture media containing desired chemoattractant to the bottom part of the Boyden chamber (bottom chamber).
- Add cell culture media containing positive control Migration Inducer to control wells in the bottom chamber.
- Add cells to the top part of the Boyden chamber (top chamber) with any desired treatment.
- Incubate at 37°C in cell culture incubator for 2-48 h.
- Cells will migrate through the polycarbonate membrane from the top chamber to the bottom chamber.
- Remove the top chamber and remove media and remaining cells.
- Centrifuge the bottom chamber and wash cells with Wash Buffer, repeat.
- Add Cell Dye / Cell Disassociation Solution to bottom chamber and incubate for 60-min in cell culture incubator.
- Analyze with fluorometric (Ex/Em 530/590 nm) plate reader.
- Prepare a separate standard curve with known numbers of cells in a 96-well plate with Cell Dye, incubate for 1-hr, and analyze with fluorometric (Ex/Em 530/590 nm) plate reader to allow quantitation.
Other notes
This product was previously called K907 Biovision EZCell™ Cell Migration/Chemotaxis Assay Kit (96-well, 5 μm). Biovision was acquired by Abcam in 2021.
Cell migration is a process by which cells move from one location to another. Cell motility is observed in unicellular organisms, and is essential for the development and maintenance of multicellular organisms. Cells often migrate in response to specific external stimuli, including chemical & mechanical signals. Errors during this process have serious consequences, including intellectual disability, vascular disease, tumor formation and metastasis.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
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2 product images
Functional Studies - Cell Migration/Chemotaxis Assay Kit (96-well, 5 µm) (ab235693)
Cell Migration.
Monocytes/macrophage cells were starved overnight and treated with Control Migration Inducer for 24 h, 48 h or left untreated (Cnt: control cells). Treatment with Control Migration Inducer demonstrated a significant increase in migration with time as compared to control cells.
Functional Studies - Cell Migration/Chemotaxis Assay Kit (96-well, 5 µm) (ab235693)
Standard Curve.
Monocytes/macrophage cells were harvested, counted and serially diluted to obtain desired cell number. Cells were incubated according to the protocol with Cell Dye and RFU (Ex/Em = 530/590 nm) was measured.
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