Cell Migration/Chemotaxis Assay Kit (96-well, 5 µm) (ab235693) uses a Boyden chamber, where the cells migrate through a semi-permeable membrane under different stimuli.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- International journal of molecular sciences 23:2022GSK343, an Inhibitor of Enhancer of Zeste Homolog 2, Reduces Glioblastoma Progression through Inflammatory Process Modulation: Focus on Canonical and Non-Canonical NF-κB/IκBα Pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah Adriana Scuderi,Alessia Filippone,Rossella Basilotta,Deborah Mannino,Giovanna Casili,Anna Paola Capra,Giulia Chisari,Lorenzo Colarossi,Serena Sava,Michela Campolo,Emanuela Esposito,Irene PaternitiPubMed 36430394
- Cell reports 35:1093032021Toll-like receptor 2 induces pathogenicity in Th17 cells and reveals a role for IPCEF in regulating Th17 cell migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKathryne E Marks,Stephanie Flaherty,Kristen M Patterson,Matthew Stratton,Gustavo J Martinez,Joseph M ReynoldsPubMed 34192530
- Cancer immunology research 9:200-2132020Targeted Deletion of CXCR2 in Myeloid Cells Alters the Tumor Immune Environment to Improve Antitumor Immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinming Yang,Chi Yan,Anna E Vilgelm,Sheau-Chiann Chen,Gregory D Ayers,Christopher A Johnson,Ann RichmondPubMed 33177110
Key facts
- Detection method
Fluorescent
- Sample types
Adherent cells, Suspension cells
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Cell Migration/Chemotaxis Assay Kit (96-well, 5 µm) (ab235693) uses a Boyden chamber, where the cells migrate through a semi-permeable membrane under different stimuli.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
Fluorescent
- Sample types
Adherent cells, Suspension cells
- Assay Platform
Microplate reader
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Cell Migration/Chemotaxis Assay Kit (96-well, 5 μm) (ab235693) utilizes a Boyden chamber, where the cells migrate through a semi-permeable membrane under different stimuli. Cell migration can be analyzed directly by reading fluorescence (Ex/Em = 530/590 nm) in a plate reader. The assay is easy to use, sensitive and adaptable to high-throughput systems.
This product is manufactured by BioVision, an Abcam company and was previously called K907 EZCell™ Cell Migration/Chemotaxis Assay Kit (96-well, 5 μm). K907-100 is the same size as the 100 test size of ab235693.
Cell migration is a process by which cells move from one location to another. Cell motility is observed in unicellular organisms, and is essential for the development and maintenance of multicellular organisms. Cells often migrate in response to specific external stimuli, including chemical & mechanical signals. Errors during this process have serious consequences, including intellectual disability, vascular disease, tumor formation and metastasis.
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Functional Studies - Cell Migration/Chemotaxis Assay Kit (96-well, 5 µm) (ab235693)
Cell Migration.
Monocytes/macrophage cells were starved overnight and treated with Control Migration Inducer for 24 h, 48 h or left untreated (Cnt: control cells). Treatment with Control Migration Inducer demonstrated a significant increase in migration with time as compared to control cells.
Functional Studies - Cell Migration/Chemotaxis Assay Kit (96-well, 5 µm) (ab235693)
Standard Curve.
Monocytes/macrophage cells were harvested, counted and serially diluted to obtain desired cell number. Cells were incubated according to the protocol with Cell Dye and RFU (Ex/Em = 530/590 nm) was measured.
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