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Cell Migration/Chemotaxis Assay Kit (96-well, 8 ?m) (ab235673) measures cell migration in response to stimuli in adherent and suspension cells.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.


Images

Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

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What's included?

100 Test
Components
Cell Chamber (96 x 8µm)
1 x 1 Unit
Cell Dissociation Solution I
1 x 15 mL
Cell Dye I
1 x 1.5 mL
Control Migration Inducer
1 x 300 µL
Wash Buffer II
1 x 50 mL

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Cell Migration/Chemotaxis Assay Kit (96-well, 8 ?m) (ab235673) measures cell migration in response to stimuli in adherent and suspension cells.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Assay Platform

Microplate reader

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Cell Migration/Chemotaxis Assay Kit (96-well, 8 μm) (ab235673) measures cell migration in response to stimuli in adherent and suspension cells. It allows you to screen, study, or characterize compounds that influence chemotaxis/cell migration. It utilizes a Boyden chamber, where the cells migrate through a semi-permeable membrane under different stimuli. Cell migration can be analyzed directly by reading fluorescence (Ex/Em = 530/590 nm) in a plate reader. Our assay is easy to use, sensitive and adaptable to high-throughput systems.

8 μm semi-permeable membrane maybe too large to use with tiny cell types, such as the cell types which diameter less than 8 μm.

This product is manufactured by BioVision, an Abcam company and was previously called K906 EZCell™ Cell Migration/Chemotaxis Assay Kit (96-well, 8 μm). K906-100 is the same size as the 100 test size of ab235673.

Cell invasion is the ability of cells to migrate from one area to another through an extracellular matrix. Cell invasion is exhibited by both normal cells as well as cancerous cells in response to specific external signals, including chemical and mechanical stimuli. During invasion, extracellular matrix is enzymatically degraded by cellular proteases before cells migrate to the new location. Cell invasion is required for normal processes such as wound repair, vasculature formation and the inflammatory response as well as the abnormal invasion of tissues by tumor cells during metastasis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Functional Studies - Cell Migration/Chemotaxis Assay Kit (96-well, 8 µm) (ab235673), expandable thumbnail

    Functional Studies - Cell Migration/Chemotaxis Assay Kit (96-well, 8 µm) (ab235673)

    Standard Curve.

    HT-1080 cells were harvested, counted and serially diluted to obtain desired cell number. Cells were incubated according to the protocol.

  • Functional Studies - Cell Migration/Chemotaxis Assay Kit (96-well, 8 µm) (ab235673), expandable thumbnail

    Functional Studies - Cell Migration/Chemotaxis Assay Kit (96-well, 8 µm) (ab235673)

    Cell Invasion.

    3T3-NIH and HT-1080 cells were starved overnight and treated with Control (Cnt) Invasion Inducer or remain untreated (No Treatment). Treatment with Control Invasion Inducer demonstrated a significant increase in invasion of HT 1080 cells as compare to 3T3-NIH control cells.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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