Cell Proliferation Staining Reagent - Blue Fluorescence (Ex 405 nm) - Cytopainter
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Functional Studies - Cell Proliferation Staining Reagent - Blue Fluorescence (Ex 405 nm) - Cytopainter (AB176726)
Emission and Excitation spectra for CytoPainter Cell Proliferation Staining Reagent - Blue Fluorescence (Ex/Em = 403/454nm) (ab176726), CytoPainter Cell Proliferation Staining Reagent - Green Fluorescence (Ex/Em = 511/525nm) (ab176735) and CytoPainter Cell Proliferation Staining Staining Reagent - Red Fluorescence (Ex/Em = 628/643) (ab176736) in PBS buffer (pH=7.2).
Product details
Cell Proliferation Staining Reagent - Blue Fluorescence (Ex 405 nm) | Cytopainter (ab176726) is a powerful tool to analyze heterogeneous cell populations where multicolor analysis is required. This reagent is designed to label cells at Ex/Em = 403/454 nm excited with a violet laser at Ex = 405 nm.
The CytoLabeling Blue Reagent dye contained in this kit is functionally similar to CFSE and can be used for the multicolor applications where either GFP or FITC-labeled antibody is used, since the CytoLabeling Reagents staining reagents have either excitation and emission spectra distinct from CFSE and its fluorescein analogs.
Main features of CytoLabeling Blue Reagent:
- It eliminates the dye efflux drawback associated with CFSE
- It is compatible with the cell culture medium in the staining cells prior analysis - no washing required
- It is retained up to 9 generations after staining
- Fluorescence is preserved upon formaldehyde fixation
This product can be used for imaging or flow cytometric analysis.
Review other dyes and kits for cell proliferation staining, or the live cell staining fluorescent dyes guide,
Uniform labeling of live cells with a dye containing a cell-retaining group to allow retention in proliferating cells. It enables multicolor labeling and functional analysis of live cells in combination with other fluorochromes. The dye is retained for up to 9 generations after staining.
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Publications (1)
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NPJ precision oncology 8:179 PubMed39143103
2024
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