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AB176726

Cell Proliferation Staining Reagent - Blue Fluorescence (Ex 405 nm) - Cytopainter

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(1 Publication)

Cell Proliferation Staining Reagent - Blue Fluorescence (Ex 405 nm) | Cytopainter (ab176726) is a powerful tool to analyze heterogeneous cell populations where multicolor analysis is required.
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Functional Studies - Cell Proliferation Staining Reagent - Blue Fluorescence (Ex 405 nm) - Cytopainter (AB176726)
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Functional Studies - Cell Proliferation Staining Reagent - Blue Fluorescence (Ex 405 nm) - Cytopainter (AB176726)

Emission and Excitation spectra for CytoPainter Cell Proliferation Staining Reagent - Blue Fluorescence (Ex/Em = 403/454nm) (ab176726), CytoPainter Cell Proliferation Staining Reagent - Green Fluorescence (Ex/Em = 511/525nm) (ab176735) and CytoPainter Cell Proliferation Staining Staining Reagent - Red Fluorescence (Ex/Em = 628/643) (ab176736) in PBS buffer (pH=7.2).

Key facts

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based

Results type

Quantitative

Assay Platform

Flow cytometer

Product details

Cell Proliferation Staining Reagent - Blue Fluorescence (Ex 405 nm) | Cytopainter (ab176726) is a powerful tool to analyze heterogeneous cell populations where multicolor analysis is required. This reagent is designed to label cells at Ex/Em = 403/454 nm excited with a violet laser at Ex = 405 nm.

The CytoLabeling Blue Reagent dye contained in this kit is functionally similar to CFSE and can be used for the multicolor applications where either GFP or FITC-labeled antibody is used, since the CytoLabeling Reagents staining reagents have either excitation and emission spectra distinct from CFSE and its fluorescein analogs.

Main features of CytoLabeling Blue Reagent:

  • It eliminates the dye efflux drawback associated with CFSE
  • It is compatible with the cell culture medium in the staining cells prior analysis - no washing required
  • It is retained up to 9 generations after staining
  • Fluorescence is preserved upon formaldehyde fixation

This product can be used for imaging or flow cytometric analysis.

Review other dyes and kits for cell proliferation staining, or the live cell staining fluorescent dyes guide,

Uniform labeling of live cells with a dye containing a cell-retaining group to allow retention in proliferating cells. It enables multicolor labeling and functional analysis of live cells in combination with other fluorochromes. The dye is retained for up to 9 generations after staining.

What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

NPJ precision oncology 8:179 PubMed39143103

2024

Probing the killing potency of tumor-infiltrating lymphocytes on microarrayed colorectal cancer tumoroids.

Applications

Unspecified application

Species

Unspecified reactive species

Devanjali Dutta,L Francisco Lorenzo-Martín,François Rivest,Nicolas Broguiere,Lucie Tillard,Simone Ragusa,Nathalie Brandenberg,Sylke Höhnel,Damien Saugy,Sylvie Rusakiewicz,Krisztian Homicsko,George Coukos,Matthias P Lutolf
View all publications
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