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Cell Proliferation Staining Reagent - Deep Red Fluorescence | Cytopainter (ab176736) is a powerful tool to analyze heterogeneous cell populations where multicolor analysis is required.


Images

Flow Cytometry - Cell Proliferation Staining Reagent - Deep Red Fluorescence - Cytopainter (AB176736), expandable thumbnail
  • Flow Cytometry - Cell Proliferation Staining Reagent - Deep Red Fluorescence - Cytopainter (AB176736), expandable thumbnail

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Key facts

Sample types
Suspension cells, Adherent cells
Assay type
Cell-based (quantitative)
Reactive species
Mammals

Associated Products

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2 products for Alternative Product

What's included?

500 Test
Components
CytoLabeling Red Reagent
1 x 1 Vial

Recommended products

Cell Proliferation Staining Reagent - Deep Red Fluorescence | Cytopainter (ab176736) is a powerful tool to analyze heterogeneous cell populations where multicolor analysis is required.

Key facts

Sample types
Suspension cells, Adherent cells
Assay type
Cell-based (quantitative)
Reactive species
Mammals
Assay Platform
Flow cytometer

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Cell Proliferation Staining Reagent - Deep Red Fluorescence | Cytopainter (ab176736) is a powerful tool to analyze heterogeneous cell populations where multicolor analysis is required. This reagent is designed to label cells at Em = 628-643 nm excited with a red laser at Ex = 633 nm.

The CytoLabeling Red Reagent dye contained in this kit is functionally similar to CFSE and can be used for the multicolor applications where either GFP or FITC-labeled antibody is used, since the CytoLabeling Reagents staining reagents have either excitation and emission spectra distinct from CFSE and its fluorescein analogs.

Main features of CytoLabeling Deep Red Reagent:

  • It eliminates the dye efflux drawback associated with CFSE
  • It is compatible with the cell culture medium in the staining cells prior analysis - no washing required
  • It is retained up to 9 generations after staining
  • Fluorescence is preserved upon formaldehyde fixation

This product can be used for imaging or flow cytometric analysis.

Review other dyes and kits for cell proliferation staining, or the live cell staining fluorescent dyes guide

Uniform labeling of live cells with a dye containing a cell-retaining group to allow retention in proliferating cells. It enables multicolor labeling and functional analysis of live cells in combination with other fluorochromes. The dye is retained for up to 9 generations after staining.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Flow Cytometry - Cell Proliferation Staining Reagent - Deep Red Fluorescence - Cytopainter (ab176736), expandable thumbnail

    Flow Cytometry - Cell Proliferation Staining Reagent - Deep Red Fluorescence - Cytopainter (ab176736)

    Emission and Excitation spectra for CytoPainter Cell Proliferation Staining Reagent - Red Fluorescence (ab176736).

  • Flow Cytometry - Cell Proliferation Staining Reagent - Deep Red Fluorescence - Cytopainter (ab176736), expandable thumbnail

    Flow Cytometry - Cell Proliferation Staining Reagent - Deep Red Fluorescence - Cytopainter (ab176736)

    Cell tracking assay with CytoPainter Cell Proliferation Staining Reagent - Red Fluorescence (ab176736). Jurkat cells (2x106 cells/mL) were stained with ab176736 on Day 0. The cells were passed serially at 1:1 ratio for 11 days. Fluorescence intensity was measured with a flow cytometer in FL1 channel on the day after passage. Successive generations were represented by different colors.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com