Cell Proliferation Staining Reagent - Deep Red Fluorescence - Cytopainter
4
(2 Reviews)
|
(15 Publications)
- Flow Cyt
Supplier Data
Flow Cytometry - Cell Proliferation Staining Reagent - Deep Red Fluorescence - Cytopainter (AB176736)
Emission and Excitation spectra for CytoPainter Cell Proliferation Staining Reagent - Red Fluorescence (ab176736).
- Flow Cyt
Supplier Data
Flow Cytometry - Cell Proliferation Staining Reagent - Deep Red Fluorescence - Cytopainter (AB176736)
Cell tracking assay with CytoPainter Cell Proliferation Staining Reagent - Red Fluorescence (ab176736). Jurkat cells (2x106 cells/mL) were stained with ab176736 on Day 0. The cells were passed serially at 1 : 1 ratio for 11 days. Fluorescence intensity was measured with a flow cytometer in FL1 channel on the day after passage. Successive generations were represented by different colors.
Product details
Cell Proliferation Staining Reagent - Deep Red Fluorescence | Cytopainter (ab176736) is a powerful tool to analyze heterogeneous cell populations where multicolor analysis is required. This reagent is designed to label cells at Em = 628-643 nm excited with a red laser at Ex = 633 nm.
The CytoLabeling Red Reagent dye contained in this kit is functionally similar to CFSE and can be used for the multicolor applications where either GFP or FITC-labeled antibody is used, since the CytoLabeling Reagents staining reagents have either excitation and emission spectra distinct from CFSE and its fluorescein analogs.
Main features of CytoLabeling Deep Red Reagent:
- It eliminates the dye efflux drawback associated with CFSE
- It is compatible with the cell culture medium in the staining cells prior analysis - no washing required
- It is retained up to 9 generations after staining
- Fluorescence is preserved upon formaldehyde fixation
This product can be used for imaging or flow cytometric analysis.
Review other dyes and kits for cell proliferation staining, or the live cell staining fluorescent dyes guide.
Uniform labeling of live cells with a dye containing a cell-retaining group to allow retention in proliferating cells. It enables multicolor labeling and functional analysis of live cells in combination with other fluorochromes. The dye is retained for up to 9 generations after staining.
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Publications (15)
Recent publications for all applications. Explore the full list and refine your search
International journal of molecular sciences 26: PubMed39859183
2025
Applications
Unspecified application
Species
Unspecified reactive species
F1000Research 12:324 PubMed39006307
2024
Applications
Unspecified application
Species
Unspecified reactive species
Cell reports. Medicine 5:101582 PubMed38781959
2024
Applications
Unspecified application
Species
Unspecified reactive species
F1000Research 12:172 PubMed38106655
2023
Applications
Unspecified application
Species
Unspecified reactive species
Frontiers in bioengineering and biotechnology 11:1159819 PubMed37346794
2023
Applications
Unspecified application
Species
Unspecified reactive species
Cancers 15: PubMed36980588
2023
Applications
Unspecified application
Species
Unspecified reactive species
Biomedicines 10: PubMed36140196
2022
Applications
Unspecified application
Species
Unspecified reactive species
International journal of biological macromolecules 215:691-704 PubMed35777518
2022
Applications
Unspecified application
Species
Unspecified reactive species
Cancers 14: PubMed35625969
2022
Applications
Unspecified application
Species
Unspecified reactive species
Cancers 14: PubMed35008299
2021
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com