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AB176736

Cell Proliferation Staining Reagent - Deep Red Fluorescence - Cytopainter

4

(2 Reviews)

|

(15 Publications)

Cell Proliferation Staining Reagent - Deep Red Fluorescence | Cytopainter (ab176736) is a powerful tool to analyze heterogeneous cell populations where multicolor analysis is required.
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Flow Cytometry - Cell Proliferation Staining Reagent - Deep Red Fluorescence - Cytopainter (AB176736)
  • Flow Cyt

Supplier Data

Flow Cytometry - Cell Proliferation Staining Reagent - Deep Red Fluorescence - Cytopainter (AB176736)

Emission and Excitation spectra for CytoPainter Cell Proliferation Staining Reagent - Red Fluorescence (ab176736).

Flow Cytometry - Cell Proliferation Staining Reagent - Deep Red Fluorescence - Cytopainter (AB176736)
  • Flow Cyt

Supplier Data

Flow Cytometry - Cell Proliferation Staining Reagent - Deep Red Fluorescence - Cytopainter (AB176736)

Cell tracking assay with CytoPainter Cell Proliferation Staining Reagent - Red Fluorescence (ab176736). Jurkat cells (2x106 cells/mL) were stained with ab176736 on Day 0. The cells were passed serially at 1 : 1 ratio for 11 days. Fluorescence intensity was measured with a flow cytometer in FL1 channel on the day after passage. Successive generations were represented by different colors.

Key facts

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based (quantitative)

Assay Platform

Flow cytometer

Product details

Cell Proliferation Staining Reagent - Deep Red Fluorescence | Cytopainter (ab176736) is a powerful tool to analyze heterogeneous cell populations where multicolor analysis is required. This reagent is designed to label cells at Em = 628-643 nm excited with a red laser at Ex = 633 nm.

The CytoLabeling Red Reagent dye contained in this kit is functionally similar to CFSE and can be used for the multicolor applications where either GFP or FITC-labeled antibody is used, since the CytoLabeling Reagents staining reagents have either excitation and emission spectra distinct from CFSE and its fluorescein analogs.

Main features of CytoLabeling Deep Red Reagent:

  • It eliminates the dye efflux drawback associated with CFSE
  • It is compatible with the cell culture medium in the staining cells prior analysis - no washing required
  • It is retained up to 9 generations after staining
  • Fluorescence is preserved upon formaldehyde fixation

This product can be used for imaging or flow cytometric analysis.

Review other dyes and kits for cell proliferation staining, or the live cell staining fluorescent dyes guide.

Uniform labeling of live cells with a dye containing a cell-retaining group to allow retention in proliferating cells. It enables multicolor labeling and functional analysis of live cells in combination with other fluorochromes. The dye is retained for up to 9 generations after staining.

What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

Publications (15)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed39859183

2025

Exploring CAR T-Cell Dynamics: Balancing Potent Cytotoxicity and Controlled Inflammation in CAR T-Cells Derived from Systemic Sclerosis and Myositis Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Janin Dingfelder,Jule Taubmann,Franziska von Heydebrand,Michael Aigner,Christina Bergmann,Johannes Knitza,Soo Park,Joseph K Cheng,Thomas Van Blarcom,Georg Schett,Andreas Mackensen,Gloria Lutzny-Geier

F1000Research 12:324 PubMed39006307

2024

The identification of high-performing antibodies for Sequestosome-1 for use in Western blot, immunoprecipitation and immunofluorescence.

Applications

Unspecified application

Species

Unspecified reactive species

Riham Ayoubi,Walaa Alshafie,Irina Shlaifer,Kathleen Southern,Peter S McPherson,Carl Laflamme

Cell reports. Medicine 5:101582 PubMed38781959

2024

Single-cell multiomics profiling reveals heterogeneous transcriptional programs and microenvironment in DSRCTs.

Applications

Unspecified application

Species

Unspecified reactive species

Clémence Henon,Julien Vibert,Thomas Eychenne,Nadège Gruel,Léo Colmet-Daage,Carine Ngo,Marlène Garrido,Nicolas Dorvault,Maria Eugenia Marques Da Costa,Virginie Marty,Nicolas Signolle,Antonin Marchais,Noé Herbel,Asuka Kawai-Kawachi,Madison Lenormand,Clémence Astier,Roman Chabanon,Benjamin Verret,Rastislav Bahleda,Axel Le Cesne,Fatima Mechta-Grigoriou,Matthieu Faron,Charles Honoré,Olivier Delattre,Joshua J Waterfall,Sarah Watson,Sophie Postel-Vinay

F1000Research 12:172 PubMed38106655

2023

Identification of high-performing antibodies for Moesin for use in Western Blot, immunoprecipitation, and immunofluorescence.

Applications

Unspecified application

Species

Unspecified reactive species

Walaa Alshafie,Riham Ayoubi,Maryam Fotouhi,Kathleen Southern,Carl Laflamme

Frontiers in bioengineering and biotechnology 11:1159819 PubMed37346794

2023

Dual CSF1R inhibition and CD40 activation demonstrates anti-tumor activity in a 3D macrophage- HER2 breast cancer spheroid model.

Applications

Unspecified application

Species

Unspecified reactive species

Manuel Rodriguez-Perdigon,Laetitia Haeni,Barbara Rothen-Rutishauser,Curzio Rüegg

Cancers 15: PubMed36980588

2023

The Chorioallantoic Membrane Xenograft Assay as a Reliable Model for Investigating the Biology of Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Raphela A Ranjan,Julienne K Muenzner,Philipp Kunze,Carol I Geppert,Matthias Ruebner,Hanna Huebner,Peter A Fasching,Matthias W Beckmann,Tobias Bäuerle,Arndt Hartmann,Wolfgang Walther,Markus Eckstein,Ramona Erber,Regine Schneider-Stock

Biomedicines 10: PubMed36140196

2022

Multi-Functionalized Heteroduplex Antisense Oligonucleotides for Targeted Intracellular Delivery and Gene Silencing in HeLa Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mauro Sousa de Almeida,Barbara Rothen-Rutishauser,Michael Mayer,Maria Taskova

International journal of biological macromolecules 215:691-704 PubMed35777518

2022

Biological activity of multicomponent bio-hydrogels loaded with tragacanth gum.

Applications

Unspecified application

Species

Unspecified reactive species

Roberta Teixeira Polez,Maria Morits,Christopher Jonkergouw,Josphat Phiri,Juan José Valle-Delgado,Markus B Linder,Thaddeus Maloney,Orlando J Rojas,Monika Österberg

Cancers 14: PubMed35625969

2022

Multiphoton Microscopy Reveals DAPK1-Dependent Extracellular Matrix Remodeling in a Chorioallantoic Membrane (CAM) Model.

Applications

Unspecified application

Species

Unspecified reactive species

Philipp Kunze,Lucas Kreiss,Vendula Novosadová,Adriana V Roehe,Sara Steinmann,Jan Prochazka,Carol I Geppert,Arndt Hartmann,Sebastian Schürmann,Oliver Friedrich,Regine Schneider-Stock

Cancers 14: PubMed35008299

2021

A Gene Signature Derived from the Loss of CDKN1A (p21) Is Associated with CMS4 Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Santiago Bueno-Fortes,Julienne K Muenzner,Alberto Berral-Gonzalez,Chuanpit Hampel,Pablo Lindner,Alexandra Berninger,Kerstin Huebner,Philipp Kunze,Tobias Bäuerle,Katharina Erlenbach-Wuensch,José Manuel Sánchez-Santos,Arndt Hartmann,Javier De Las Rivas,Regine Schneider-Stock
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