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AB176735

Cell Proliferation Staining Reagent - Green Fluorescence - Cytopainter

5

(2 Reviews)

|

(12 Publications)

Cell Proliferation Staining Reagent - Green Fluorescence | Cytopainter (ab176735) is a powerful tool to analyze heterogeneous cell populations where multicolor analysis is required.
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Flow Cytometry - Cell Proliferation Staining Reagent - Green Fluorescence - Cytopainter (AB176735)
  • Flow Cyt

Supplier Data

Flow Cytometry - Cell Proliferation Staining Reagent - Green Fluorescence - Cytopainter (AB176735)

Cell tracking assay with CytoPainter Cell Proliferation Staining Reagent - Green Fluorescence (ab176735) and CFSE. Jurkat cells (2x106 cells/mL) were stained with ab176735 or CFSE (0.5 μM) on Day 0. The cells were passed serially at 1 : 1 ratio for 9 days. Fluorescence intensity was measured with a flow cytometer in FL1 channel on the day after passage. Successive generations were represented by different colors.

Functional Studies - Cell Proliferation Staining Reagent - Green Fluorescence - Cytopainter (AB176735)
  • FuncS

Supplier Data

Functional Studies - Cell Proliferation Staining Reagent - Green Fluorescence - Cytopainter (AB176735)

Emission and Excitation spectra for CytoPainter Cell Proliferation Staining Reagent – Green Fluorescence (ab176735).

Key facts

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based

Results type

Quantitative

Assay Platform

Microplate reader, Flow cytometer

Product details

Cell Proliferation Staining Reagent - Green Fluorescence | Cytopainter (ab176735) is a powerful tool to analyze heterogeneous cell populations where multicolor analysis is required. The CytoLabeling Green Reagent is designed to label cells at Em = 511-525 nm excited with a blue laser at Ex = 488 nm.

The CytoLabeling Green Reagent dye contained in this kit is functionally similar to CFSE and can be used for the multicolor applications where either GFP or FITC-labeled antibody is used.

Main features of CytoLabeling Green Reagent:

  • It eliminates the dye efflux drawback associated with CFSE
  • It is compatible with the cell culture medium in the staining cells prior analysis - no washing required
  • It is retained up to 9 generations after staining
  • Fluorescence is preserved upon formaldehyde fixation

This product can be used for imaging or flow cytometric analysis.

Review other dyes and kits for cell proliferation staining, or the live cell staining fluorescent dyes guide.

Uniform labeling of live cells with a dye containing a cell-retaining group to allow retention in proliferating cells. It enables multicolor labeling and functional analysis of live cells in combination with other fluorochromes. The dye is retained for up to 9 generations after staining.

What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

Publications (12)

Recent publications for all applications. Explore the full list and refine your search

EJNMMI physics 12:41 PubMed40249448

2025

Do cell culturing influence the radiosensitizing effect of gold nanoparticles: a Monte Carlo study.

Applications

Unspecified application

Species

Unspecified reactive species

J Antunes,T Pinheiro,I Marques,S Pires,M Filomena Botelho,J M Sampaio,A Belchior

Stem cell research & therapy 15:265 PubMed39183328

2024

Salivary gland organoid transplantation as a therapeutic option for radiation-induced xerostomia.

Applications

Unspecified application

Species

Unspecified reactive species

Seong Gyeong Jeon,Jaeseon Lee,Su Jeong Lee,Jaehwi Seo,Jinkyoung Choi,Dong Hyuck Bae,Duk-Hee Chun,Seung Young Ko,Hyun Soo Shin,Lina Joo,Sang-Hyuk Lee,Young Chang Lim,Woo Hee Choi,Jongman Yoo

F1000Research 12:324 PubMed39006307

2024

The identification of high-performing antibodies for Sequestosome-1 for use in Western blot, immunoprecipitation and immunofluorescence.

Applications

Unspecified application

Species

Unspecified reactive species

Riham Ayoubi,Walaa Alshafie,Irina Shlaifer,Kathleen Southern,Peter S McPherson,Carl Laflamme

International journal of molecular sciences 25: PubMed38396992

2024

Differential Expression of Stress Adaptation Genes in a Diatom under Different Culture Conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Elvira Bayramova,Darya Petrova,Artyom Marchenkov,Alexey Morozov,Yuri Galachyants,Yulia Zakharova,Yekaterina Bedoshvili,Yelena Likhoshway

Biomaterials science 12:1738-1749 PubMed38372031

2024

Gelatine-collagen photo-crosslinkable 3D matrixes for skin regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Gauthier Menassol,Boudewijn van der Sanden,Laetitia Gredy,Capucine Arnol,Thibaut Divoux,Donald K Martin,Olivier Stephan

F1000Research 12:172 PubMed38106655

2023

Identification of high-performing antibodies for Moesin for use in Western Blot, immunoprecipitation, and immunofluorescence.

Applications

Unspecified application

Species

Unspecified reactive species

Walaa Alshafie,Riham Ayoubi,Maryam Fotouhi,Kathleen Southern,Carl Laflamme

Communications biology 6:1157 PubMed37957280

2023

A fully 3D-printed versatile tumor-on-a-chip allows multi-drug screening and correlation with clinical outcomes for personalized medicine.

Applications

Unspecified application

Species

Unspecified reactive species

Eliana Steinberg,Roy Friedman,Yoel Goldstein,Nethanel Friedman,Ofer Beharier,Jonathan Abraham Demma,Gideon Zamir,Ayala Hubert,Ofra Benny

Cells 10: PubMed33807701

2021

Generation and Evaluation of Isogenic iPSC as a Source of Cell Replacement Therapies in Patients with Kearns Sayre Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Glen Lester Sequiera,Abhay Srivastava,Keshav Narayan Alagarsamy,Cheryl Rockman-Greenberg,Sanjiv Dhingra

Assay and drug development technologies : PubMed33471566

2021

Profiling the Neoplasm Microenvironment of Silica Nanomaterial-Derived Scaffolds of Single, 2-, and 3-Composite Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Victor Akpe,Shweta Murhekar,Tak H Kim,Christopher L Brown,Ian E Cock

Assay and drug development technologies 19:46-61 PubMed33443468

2021

Batch Effect Adjustment to Lower the Drug Attrition Rate of MCF-7 Breast Cancer Cells Exposed to Silica Nanomaterial-Derived Scaffolds.

Applications

Unspecified application

Species

Unspecified reactive species

Victor Akpe,Shweta Murhekar,Tak H Kim,Christopher L Brown,Ian E Cock
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