Cell Proliferation Staining Reagent - Green Fluorescence | Cytopainter (ab176735) is a powerful tool to analyze heterogeneous cell populations where multicolor analysis is required.
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- F1000Research 12:1722023Identification of high-performing antibodies for Moesin for use in Western Blot, immunoprecipitation, and immunofluorescence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWalaa Alshafie,Riham Ayoubi,Maryam Fotouhi,Kathleen Southern,Carl LaflammePubMed 38106655
- Communications biology 6:11572023A fully 3D-printed versatile tumor-on-a-chip allows multi-drug screening and correlation with clinical outcomes for personalized medicine.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEliana Steinberg,Roy Friedman,Yoel Goldstein,Nethanel Friedman,Ofer Beharier,Jonathan Abraham Demma,Gideon Zamir,Ayala Hubert,Ofra BennyPubMed 37957280
- Cells 10:2021Generation and Evaluation of Isogenic iPSC as a Source of Cell Replacement Therapies in Patients with Kearns Sayre Syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGlen Lester Sequiera,Abhay Srivastava,Keshav Narayan Alagarsamy,Cheryl Rockman-Greenberg,Sanjiv DhingraPubMed 33807701
- Assay and drug development technologies :2021Profiling the Neoplasm Microenvironment of Silica Nanomaterial-Derived Scaffolds of Single, 2-, and 3-Composite Systems.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVictor Akpe et. al.PubMed 33471566
- Assay and drug development technologies 19:46-612021Batch Effect Adjustment to Lower the Drug Attrition Rate of MCF-7 Breast Cancer Cells Exposed to Silica Nanomaterial-Derived Scaffolds.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVictor Akpe,Shweta Murhekar,Tak H Kim,Christopher L Brown,Ian E CockPubMed 33443468
- Neurobiology of disease 134:1046742019Fibrotic scar after experimental autoimmune encephalomyelitis inhibits oligodendrocyte differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStephanie L Yahn,Jiajun Li,Irene Goo,Han Gao,Roberta Brambilla,Jae K LeePubMed 31731043
- Nature 565:659-6632019Autophagic cell death restricts chromosomal instability during replicative crisis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoe Nassour et. al.PubMed 30675059
Key facts
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Cell-based (quantitative)
- Reactive species
- Mammals
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Cell Proliferation Staining Reagent - Green Fluorescence | Cytopainter (ab176735) is a powerful tool to analyze heterogeneous cell populations where multicolor analysis is required.
Key facts
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Cell-based (quantitative)
- Reactive species
- Mammals
- Assay Platform
- Microplate reader, Flow cytometer
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Cell Proliferation Staining Reagent - Green Fluorescence | Cytopainter (ab176735) is a powerful tool to analyze heterogeneous cell populations where multicolor analysis is required. The CytoLabeling Green Reagent is designed to label cells at Em = 511-525 nm excited with a blue laser at Ex = 488 nm.
The CytoLabeling Green Reagent dye contained in this kit is functionally similar to CFSE and can be used for the multicolor applications where either GFP or FITC-labeled antibody is used.
Main features of CytoLabeling Green Reagent:
- It eliminates the dye efflux drawback associated with CFSE
- It is compatible with the cell culture medium in the staining cells prior analysis - no washing required
- It is retained up to 9 generations after staining
- Fluorescence is preserved upon formaldehyde fixation
This product can be used for imaging or flow cytometric analysis.
Review other dyes and kits for cell proliferation staining, or the live cell staining fluorescent dyes guide
Uniform labeling of live cells with a dye containing a cell-retaining group to allow retention in proliferating cells. It enables multicolor labeling and functional analysis of live cells in combination with other fluorochromes. The dye is retained for up to 9 generations after staining.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Functional Studies - Cell Proliferation Staining Reagent - Green Fluorescence - Cytopainter (ab176735)
Emission and Excitation spectra for CytoPainter Cell Proliferation Staining Reagent – Green Fluorescence (ab176735).
Flow Cytometry - Cell Proliferation Staining Reagent - Green Fluorescence - Cytopainter (ab176735)
Cell tracking assay with CytoPainter Cell Proliferation Staining Reagent - Green Fluorescence (ab176735) and CFSE. Jurkat cells (2x106 cells/mL) were stained with ab176735 or CFSE (0.5 μM) on Day 0. The cells were passed serially at 1:1 ratio for 9 days. Fluorescence intensity was measured with a flow cytometer in FL1 channel on the day after passage. Successive generations were represented by different colors.
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