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AB176737

Cell Proliferation Staining Reagent - Orange Fluorescence - Cytopainter

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(1 Publication)

Cell Proliferation Staining Reagent - Orange Fluorescence | Cytopainter (ab176737) is a powerful tool to analyze heterogeneous cell populations where multicolor analysis is required.

Key facts

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based

Results type

Quantitative

Assay Platform

Flow cytometer

Product details

Cell Proliferation Staining Reagent - Orange Fluorescence | Cytopainter (ab176737) is a powerful tool to analyze heterogeneous cell populations where multicolor analysis is required. This reagent is designed to label cells at Em = 542-556 nm excited with a blue laser at Ex = 488 nm or with a green laser at Ex = 531 nm.

The CytoLabeling Orange Reagent dye contained in this kit is functionally similar to CFSE and can be used for the multicolor applications where either GFP or FITC-labeled antibody is used, since the CytoLabeling Reagents staining reagents have either excitation and emission spectra distinct from CFSE and its fluorescein analogs.

Main features of CytoLabeling Orange Reagent:

  • It eliminates the dye efflux drawback associated with CFSE
  • It is compatible with the cell culture medium in the staining cells prior analysis - no washing required
  • It is retained up to 9 generations after staining
  • Fluorescence is preserved upon formaldehyde fixation

This product can be used for imaging or flow cytometric analysis.

Review other dyes and kits for cell proliferation staining, or the live cell staining fluorescent dyes guide.

Uniform labeling of live cells with a dye containing a cell-retaining group to allow retention in proliferating cells. It enables multicolor labeling and functional analysis of live cells in combination with other fluorochromes. The dye is retained for up to 9 generations after staining.

What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 8:524 PubMed28523003

2017

HPV16-E7-Specific Activated CD8 T Cells in E7 Transgenic Skin and Skin Grafts.

Applications

Unspecified application

Species

Unspecified reactive species

Seyed Davoud Jazayeri,Paula T Kuo,Graham Robert Leggatt,Ian H Frazer
View all publications
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