JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB235698

Cell Transformation Assay Kit (Colorimetric)

Be the first to review this product! Submit a review

|

(3 Publications)

Cell Transformation Assay Kit ab235698 is an add-and-read assay and is faster and more sensitive than the traditional soft-agar transformation assay. Instead of relying on colony counting, the assay uses conversion of the tetrazolium salt (WST) to formazan by cellular mitochondrial dehydrogenases, with signal proportional to the number of living cells. Readout is by colorimetric (450 nm) plate reader.
1 Images
Functional Studies - Cell Transformation Assay Kit (Colorimetric) (AB235698)
  • FuncS

Supplier Data

Functional Studies - Cell Transformation Assay Kit (Colorimetric) (AB235698)

Typical assay data.

(A) HeLa (human epithelial cell line from cervix adenocarcinoma) cell-dose curve; (B) Equal numbers of NIH/3T3 (mouse embryo fibroblast cell line) and HeLa cells were seeded for the Cell Transformation Assay. After 6 days, the cell numbers were measured. Standard and Samples readings were taken 4 hours after adding WST Working Solution; (C) Image of HeLa cell colonies. HeLa cells were cultured for 6 days according to the kit protocol.

Key facts

Detection method

Colorimetric

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based

Results type

Quantitative

Assay Platform

Microplate reader

style
left-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
style
left-column

Product details

Transformation of normal cells into neoplastic (malignant) cells is the first step in tumorigenesis. In vitro assays of cellular oncotransformation, which measures phenotypic alterations to cultured cells that mirror those observed in tumors, are a critical tool in studying the mechanisms of carcinogenesis. Cell transformation assays are frequently employed in toxicology to evaluate the carcinogenic potential of a particular compound. The Soft-Agar Assay is a traditional method for screening cell transformation in vitro. In this assay, transformed cultured cells gain the ability to proliferate through the soft agarose gel without attaching to a surface and tend to form spheroid-like clumps of cells (colonies). However, this method is lengthy (3-4 weeks incubation), laborious (counting colonies) and inconsistent (due to subjective counting). Cell Transformation Assay Kit (Colorimetric)(ab235698) is faster, stable and more sensitive than the traditional Soft-Agar Assay. The kit is based on the conversion of the tetrazolium salt (WST) to formazan by cellular mitochondrial dehydrogenases. The generated signal is directly proportional to the number of living cells. This one-step method is non-radioactive and simple (just add-and-read, does not require harvesting cells, and solubilization steps). The assay is high-throughput adaptable and has a wide linear range from 10,000-400,000 cells. The entire Cell Transformation Assay can be finished within 7-8 days.

This product is manufactured by BioVision, an Abcam company and was previously called K921 Cell Transformation Assay Kit (Colorimetric). K921-100 is the same size as the 100 test size of ab235698.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

style
left-column

What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab235698-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"WST Reagent III", "number":"AB235698-CMP05", "productcode":"" }, { "size":"1 x 1 mL", "name":"Staining Solution", "number":"AB235698-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Agarose Powder", "number":"AB235698-CMP01", "productcode":"" }, { "size":"2 x 1.5 mL", "name":"10X DMEM Solution", "number":"AB235698-CMP03", "productcode":"" }, { "size":"1 x 1.8 mL", "name":"Electro Coupling Solution", "number":"AB235698-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cells 13: PubMed39404414

2024

Kisspeptin Alleviates Human Hepatic Fibrogenesis by Inhibiting TGFβ Signaling in Hepatic Stellate Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kavita Prasad,Dipankar Bhattacharya,Shams Gamal Eldin Shams,Kimberly Izarraras,Tia Hart,Brent Mayfield,Maryjka B Blaszczyk,Zhongren Zhou,Utpal B Pajvani,Scott L Friedman,Moshmi Bhattacharya

Journal of experimental & clinical cancer research : CR 41:37 PubMed35081981

2022

Human embryonic mesenchymal lung-conditioned medium promotes differentiation to myofibroblast and loss of stemness phenotype in lung adenocarcinoma cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Jordi Canals,Alfons Navarro,Cristina Vila,Josep M Canals,Tania Díaz,Melissa Acosta-Plasencia,Coralí Cros-Font,Bing Han,Yangyi He,Mariano Monzó

Nature communications 12:4624 PubMed34330897

2021

AKT3-mediated IWS1 phosphorylation promotes the proliferation of EGFR-mutant lung adenocarcinomas through cell cycle-regulated U2AF2 RNA splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Georgios I Laliotis,Evangelia Chavdoula,Maria D Paraskevopoulou,Abdul Kaba,Alessandro La Ferlita,Satishkumar Singh,Vollter Anastas,Keith A Nair,Arturo Orlacchio,Vasiliki Taraslia,Ioannis Vlachos,Marina Capece,Artemis Hatzigeorgiou,Dario Palmieri,Christos Tsatsanis,Salvatore Alaimo,Lalit Sehgal,David P Carbone,Vincenzo Coppola,Philip N Tsichlis
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com