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Abcam's Cell Viability assay kits are a set of tools for monitoring cell viability and cellular functions.

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Functional Studies - Cell Viability Assay Kit (Fluorometric - Dual Green/Red) (AB112121), expandable thumbnail

Key facts

Detection method
Fluorescent
Sample types
Suspension cells, Adherent cells
Assay type
Quantitative
Reactive species
Mammals

What's included?

2 x 96 Tests
Components
Assay Buffer
1 x 20 mL
CellGreen fluorescent dye
2 x 1 Vial
DMSO
1 x 100 µL
Propidium Iodide
1 x 40 µL

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Abcam's Cell Viability assay kits are a set of tools for monitoring cell viability and cellular functions.

Key facts

Detection method
Fluorescent
Sample types
Suspension cells, Adherent cells
Assay type
Quantitative
Reactive species
Mammals
Assay Platform
Microplate reader, Fluor. microscope, Flow cyt.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Abcam's Cell Viability assay kits are a set of tools for monitoring cell viability and cellular functions. ab112121 uses two non-fluorescent indicators: The CellGreen fluorescent dye for viable cells and a cell-impermeable DNA-binding dye for the cells with compromised membranes. The non-fluorescent Green Indicator Dye can easily permeate intact live cells and is hydrolyzed by intracellular esterase to generate the strongly fluorescent hydrophilic CellGreen fluorescent dye which is well-retained in the cell cytoplasm. The esterase activity is proportional to the number of viable cells. The DNA-binding dye is quite polar and impermeable for viable cells that have intact membranes. It becomes fluorescent only upon binding to the DNA of dead cells. Cells grown in black-wall plates can be stained and quantified in less than two hours.

ab112121 is more robust and accurate than the other viability assays. It can be readily adapted for a wide variety of fluorescence platforms such as microplate assays, fluorescence microscope, and flow cytometry. The kit provides all the essential components with an optimized assay protocol. It is suitable for both proliferating and non-proliferating cells (either suspension or adherent cells). ab112121 comes with sufficient reagents to perform either 200 assays (96-well format) or 800 assays (a 384-well format).

ab112121 should be stored desiccated.Assay kit comes with sufficient reagents to perform either 200 assays (96-well format) or 800 assays (a 384-well format).**Related assays**Review the to learn about kits to perform a , and .

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1 product image

  • Functional Studies - Cell Viability Assay Kit (Fluorometric - Dual Green/Red) (ab112121), expandable thumbnail

    Functional Studies - Cell Viability Assay Kit (Fluorometric - Dual Green/Red) (ab112121)

    CHO-K1 cell number response was measured with ab112121. CHO-K1 cells at 0 to 5,000 cells/well/100 µL were seeded overnight in a black wall/clear bottom 96-well plate. The cells were incubated with 100 µL/well of Green dye-loading solution for 1 hour at 37 °C. The fluorescence intensity was measured at Ex/Em = 490/ 525 nm. The fluorescence intensity was linear (R2 = 1) to the cell number as indicated. The detection limit was 30 cells/well (n=6).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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