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AB242300

Cellular Antioxidant Assay Kit

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(1 Publication)

Cellular Antioxidant Assay Kit (ab242300) is a cell-based assay for measuring antioxidant activity within adherent cells.
3 Images
Functional Studies - Cellular Antioxidant Assay Kit (AB242300)
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Supplier Data

Functional Studies - Cellular Antioxidant Assay Kit (AB242300)

Dose-Response Curve of Quercetin Standard

Functional Studies - Cellular Antioxidant Assay Kit (AB242300)
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Supplier Data

Functional Studies - Cellular Antioxidant Assay Kit (AB242300)

Example Data for Calculation of AUC for Antioxidant Sample and Negative Control

Cellular Activity - Cellular Antioxidant Assay Kit (AB242300)
  • Cellular Activity

Supplier Data

Cellular Activity - Cellular Antioxidant Assay Kit (AB242300)

Cellular Antioxidant Activity of Quercetin in HeLa Cells.

60,000 HeLa cells were seeded and cultured in a 96-well plate until confluent. Cells were then pretreated with DCFH-DA and Quercetin for 60 minutes at 37°C. Free Radical Initiator was then added to the cells to begin the assay, which was read every five minutes for 1 hour at 37°C.

Key facts

Detection method

Fluorescent

Sample types

Adherent cells

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Product details

Cellular Antioxidant Assay Kit (ab242300) is a cell-based assay for measuring antioxidant activity within adherent cells.

Cells are first cultured in a 96-well black fluorescence cell culture plate until confluent. Then the cells are pre-incubated with a cell-permeable DCFH-DA fluorescence probe dye and the bioflavonoid Quercetin, or the antioxidant sample being tested. After a brief incubation, the cells are washed, and the reaction started by adding the Free Radical Initiator. The Free Radical Initiator creates free radicals that convert the probe to highly fluorescent DCF. The Quercetin inhibits the formation of free radicals, and thus DCF formation, in a concentration dependent manner.

Fluorescence is measured over time in a standard microplate fluorometer. This fluorescence correlates to the Quercetin's ability to quench free radicals. Test antioxidant values can be compared to Quercetin to determine antioxidant activity within the cell.

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What's included?

{ "values": { "192Test": { "sellingSize": "192 Test", "publicAssetCode":"ab242300-192Test", "assetComponentDetails": [ { "size":"2 x 1 Unit", "name":"96-Well Cell Culture Microtiter Plate", "number":"AB242300-CMP01", "productcode":"" }, { "size":"1 x 100 µL", "name":"DCFH-DA Probe (1000X)", "number":"AB242300-CMP02", "productcode":"" }, { "size":"1 x 250 µL", "name":"Quercetin", "number":"AB242300-CMP04", "productcode":"" }, { "size":"1 x 200 mg", "name":"Free Radical Initiator", "number":"AB242300-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

See full target information Benzoic acid, 2-[3,6-bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]-
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nanomaterials (Basel, Switzerland) 13: PubMed36903823

2023

An Analysis of the Toxicity, Antioxidant, and Anti-Cancer Activity of Cinnamon Silver Nanoparticles in Comparison with Extracts and Fractions of Cinnamomum Cassia at Normal and Cancer Cell Levels.

Applications

Unspecified application

Species

Unspecified reactive species

Y G El-Baz,A Moustafa,M A Ali,G E El-Desoky,S M Wabaidur,M M Faisal
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