Cellular Antioxidant Assay Kit (ab242300) is a cell-based assay for measuring antioxidant activity within adherent cells.
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- Nanomaterials (Basel, Switzerland) 13:2023An Analysis of the Toxicity, Antioxidant, and Anti-Cancer Activity of Cinnamon Silver Nanoparticles in Comparison with Extracts and Fractions of Cinnamomum Cassia at Normal and Cancer Cell Levels.Applications:Unspecified applicationReactive species:Unspecified reactive speciesY G El-Baz,A Moustafa,M A Ali,G E El-Desoky,S M Wabaidur,M M FaisalPubMed 36903823
Key facts
- Detection method
- Fluorescent
- Sample types
- Adherent cells
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Cellular Antioxidant Assay Kit (ab242300) is a cell-based assay for measuring antioxidant activity within adherent cells.
Key facts
- Detection method
- Fluorescent
- Sample types
- Adherent cells
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Cellular Antioxidant Assay Kit (ab242300) is a cell-based assay for measuring antioxidant activity within adherent cells.
Cells are first cultured in a 96-well black fluorescence cell culture plate until confluent. Then the cells are pre-incubated with a cell-permeable DCFH-DA fluorescence probe dye and the bioflavonoid Quercetin, or the antioxidant sample being tested. After a brief incubation, the cells are washed, and the reaction started by adding the Free Radical Initiator. The Free Radical Initiator creates free radicals that convert the probe to highly fluorescent DCF. The Quercetin inhibits the formation of free radicals, and thus DCF formation, in a concentration dependent manner.
Fluorescence is measured over time in a standard microplate fluorometer. This fluorescence correlates to the Quercetin's ability to quench free radicals. Test antioxidant values can be compared to Quercetin to determine antioxidant activity within the cell.
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Cellular Activity - Cellular Antioxidant Assay Kit (ab242300)
Cellular Antioxidant Activity of Quercetin in HeLa Cells.
60,000 HeLa cells were seeded and cultured in a 96-well plate until confluent. Cells were then pretreated with DCFH-DA and Quercetin for 60 minutes at 37°C. Free Radical Initiator was then added to the cells to begin the assay, which was read every five minutes for 1 hour at 37°C.
Functional Studies - Cellular Antioxidant Assay Kit (ab242300)
Functional Studies - Cellular Antioxidant Assay Kit (ab242300)
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