Cellular ROS Assay Kit (Deep Red)

Product details

Cellular ROS Assay Kit (Deep Red) ab186029 uses an ROS sensor to quantify ROS in live cells. The ROS Deep Red dye is cell-permeable and generates Deep Red fluorescence when it reacts with ROS. The fluorescence signal of the ROS Deep Red Dye can be measured by fluorescence microscopy, high-content imaging, microplate fluorometry, or flow cytometry.

ab186029 provides a sensitive fluorometric, one-step assay to detect intracellular ROS (especially superoxide and hydroxyl radical) in live cells within 1 hour. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format using either a fluorescence microplate reader at Ex/Em = 650/675 nm or a fluorescent microscope with with Cy5 filter.

Previously called Cellular Reactive Oxygen Species Detection Assay Kit (Deep Red Fluorescence).

Reactive oxygen species (ROS) are natural byproducts of the normal metabolism of oxygen and play important roles in cell signaling. However, during oxidative stress-related states, ROS levels can increase dramatically. The accumulation of ROS results in significant damage to cell structures. The role of oxidative stress in cardiovascular disease, diabetes, osteoporosis, stroke, inflammatory diseases, a number of neurodegenerative diseases and cancer has been well established. The ROS measurement will help to determine how oxidative stress modulates varied intracellular pathways.

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Review the oxidative stress marker and assay guide, or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.

To measure reactive oxygen species within cells, we recommend DCFDA / H2DCFDA - Cellular ROS Assay Kit ab113851. Alternative ROS assays are available in orange (ab186028), red (ab186027), and deep red (ab186029).  ab238535 is used to measure ROS in biofluids, culture supernatants and cell lysates.

For assays designed to differentiate ROS, superoxides, and reactive nitrogen species: to assay ROS and superoxides use ab139476; to assay ROS, superoxides, and reactive nitrogen species use ab139473; to assay superoxides use ab219943.