Cellular ROS Assay Kit (Deep Red) ab186029 uses a Reactive oxygen species (ROS) sensor to quantify ROS in live cells.
- Sensitive one-step fluorimetric assay to detect intracellular ROS (especially superoxide and hydroxyl radical) in live cells within 1 hour incubation
- Suitable for microplate reader and fluorescence microscope
- Flexible formats: use with 96-well or 384-well microtiter plate
- Cited in over 55 publications
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- Aging cell 22:e139662023In need of age-appropriate cardiac models: Impact of cell age on extracellular matrix therapy outcomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesS Gulberk Ozcebe,Pinar ZorlutunaPubMed 37803909
- Immunology 171:60-762023Suppression of protein quality control system by TRIM30a sensitises tumour cells to NK cell-mediated immune surveillance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLukman O Afolabi,Jiacheng Bi,Liang Chen,Xiaolu Yang,Xiaochun WanPubMed 37753964
- Journal of translational medicine 21:5212023UCP1 alleviates renal interstitial fibrosis progression through oxidative stress pathway mediated by SIRT3 protein stability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Xiong,Zhiyong Xiong,Anni Song,Chuntao Lei,Chen Ye,Hua Su,Chun ZhangPubMed 37533052
- Journal of orthopaedic translation 40:116-1312023Gli1 depletion induces oxidative stress and apoptosis of nucleus pulposus cells via Fos in intervertebral disc degeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLibangxi Liu,Yuyao Zhang,Jiawei Fu,Xuezheng Ai,Dan Long,Xue Leng,Yang Zhang,Bo Huang,Changqing Li,Yue Zhou,Chencheng FengPubMed 37457313
- Cell reports. Medicine 4:1010022023N-acetylcysteine overcomes NF1 loss-driven resistance to PI3Kα inhibition in breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPriska Auf der Maur,Marcel P Trefny,Zora Baumann,Milica Vulin,Ana Luisa Correia,Maren Diepenbruck,Nicolas Kramer,Katrin Volkmann,Bogdan-Tiberius Preca,Pedro Ramos,Cedric Leroy,Tobias Eichlisberger,Katarzyna Buczak,Federica Zilli,Ryoko Okamoto,Roland Rad,Michael Rugaard Jensen,Christine Fritsch,Alfred Zippelius,Michael B Stadler,Mohamed Bentires-AljPubMed 37044095
- Molecules (Basel, Switzerland) 28:2023One-Pot Synthesis and Evaluation of Antioxidative Stress and Anticancer Properties of an Active Chromone Derivative.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChirattikan Maicheen,Chokchaloemwat Churnthammakarn,Nichapat Pongsroypech,Thitiphong Khamkhenshorngphanuch,Jiraporn Ungwitayatorn,Kanin Rangsangthong,Rathapon Asasutjarit,Sewan TheeramunkongPubMed 37049900
- Frontiers in pharmacology 13:10804122023Venlafaxine, an anti-depressant drug, induces apoptosis in MV3 human melanoma cells through JNK1/2-Nur77 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing Niu,Zhiying Wei,Jiao Fu,Shu Chen,Ru Wang,Yuya Wang,Ruihe ZhengPubMed 36686679
- Nature communications 13:75222022The dynamic clustering of insulin receptor underlies its signaling and is disrupted in insulin resistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlessandra Dall'Agnese,Jesse M Platt,Ming M Zheng,Max Friesen,Giuseppe Dall'Agnese,Alyssa M Blaise,Jessica B Spinelli,Jonathan E Henninger,Erin N Tevonian,Nancy M Hannett,Charalampos Lazaris,Hannah K Drescher,Lea M Bartsch,Henry R Kilgore,Rudolf Jaenisch,Linda G Griffith,Ibrahim I Cisse,Jacob F Jeppesen,Tong I Lee,Richard A YoungPubMed 36473871
- International journal of nanomedicine 17:4449-44682022Engineered Hybrid Treg-Targeted Nanosomes Restrain Lung Immunosuppression by Inducing Intratumoral CD8T Cell Immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKalliopi Domvri,Savvas Petanidis,Paul Zarogoulidis,Doxakis Anestakis,Charalampos Charalampidis,Drosos Tsavlis,Haidong Huang,Lutz Freitag,Wolfgang Hohenforst-Schmidt,Dimitris Matthaios,Theodora Katopodi,Konstantinos PorpodisPubMed 36172007
- Frontiers in pharmacology 13:9306142022Ferroptosis induced by iron overload promotes fibrosis in ovarian endometriosis and is related to subpopulations of endometrial stromal cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanqin Zhang,Xinyu Liu,Mengqi Deng,Chunyu Xu,Yubo Zhang,Di Wu,Fan Tang,Ruiye Yang,Jinwei MiaoPubMed 36120348
Key facts
- Sample types
- Suspension cells, Adherent cells
- Assay time
- 1h
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Cellular ROS Assay Kit (Deep Red) ab186029 uses a Reactive oxygen species (ROS) sensor to quantify ROS in live cells.
- Sensitive one-step fluorimetric assay to detect intracellular ROS (especially superoxide and hydroxyl radical) in live cells within 1 hour incubation
- Suitable for microplate reader and fluorescence microscope
- Flexible formats: use with 96-well or 384-well microtiter plate
- Cited in over 55 publications
Key facts
- Sample types
- Suspension cells, Adherent cells
- Assay time
- 1h
- Assay Platform
- Microplate reader, Fluor. microscope, Flow cyt.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Cellular ROS Assay Kit (Deep Red) ab186029 uses an ROS sensor to quantify ROS in live cells. The ROS Deep Red dye is cell-permeable and generates Deep Red fluorescence when it reacts with ROS. The fluorescence signal of the ROS Deep Red Dye can be measured by fluorescence microscopy, high-content imaging, microplate fluorometry, or flow cytometry.
ab186029 provides a sensitive fluorometric, one-step assay to detect intracellular ROS (especially superoxide and hydroxyl radical) in live cells within 1 hour. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format using either a fluorescence microplate reader at Ex/Em = 650/675 nm or a fluorescent microscope with with Cy5 filter.
Previously called Cellular Reactive Oxygen Species Detection Assay Kit (Deep Red Fluorescence).Reactive oxygen species (ROS) are natural byproducts of the normal metabolism of oxygen and play important roles in cell signaling. However, during oxidative stress-related states, ROS levels can increase dramatically. The accumulation of ROS results in significant damage to cell structures. The role of oxidative stress in cardiovascular disease, diabetes, osteoporosis, stroke, inflammatory diseases, a number of neurodegenerative diseases and cancer has been well established. The ROS measurement will help to determine how oxidative stress modulates varied intracellular pathways.**Related products**Review the , or the full to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.To measure reactive oxygen species within cells, we recommend . Alternative ROS assays are available in orange (Cellular ROS Assay Kit (Orange) ab186028), red (Cellular ROS Assay Kit (Red) ab186027), and deep red (ab186029). DCF ROS/RNS Assay Kit (biofluids, culture supernatant, cell lysates) ab238535 is used to measure ROS in biofluids, culture supernatants and cell lysates. For assays designed to differentiate ROS, superoxides, and reactive nitrogen species: to assay ROS and superoxides use ROS/Superoxide Detection Assay Kit (Cell-based) ab139476; to assay ROS, superoxides, and reactive nitrogen species use Cellular ROS/RNS Assay Kit ab139473; to assay superoxides use Mitochondrial Superoxide Assay Kit (Fluorometric) ab219943.
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Functional Studies - Cellular ROS Assay Kit (Deep Red) (ab186029)
Images of Hela cells stained with the Cellular Reactive Oxygen Species Detection Assay Kit (Deep Red Fluorescence) (ab186029) in a black wall/clear bottom 96-well plate. A: Untreated control cells. B: Cells treated with 100 μM TBHP for 30min before staining.
Functional Studies - Cellular ROS Assay Kit (Deep Red) (ab186029)
Detection of ROS in Jurkat Cells.
Jurkat cells were treated without (Blue) or with 100μM TBHP (Red) for 30min at 37 °C, and then loaded with ROS Deep Red in a 5% CO2, 37 °C incubator for 1 hour. The fluorescent intensities were measured with a flow cytometer using FL2 channel
Functional Studies - Cellular ROS Assay Kit (Deep Red) (ab186029)
Detection of ROS in Hela cells.
Hela cells were seeded overnight at 15,000 cells/90 μl/well in a black wall/clear bottom 96-well plate. The cells were untreated (control) or treated with1 mM H2O2 or 100 μM TBHP for 30 minutes at 37 °C. The ROS Deep Red assay solution (100 μl/well) was added and incubated in a 5% CO2, 37°C incubator for 1 hour. The fluorescence signal was monitored at Ex/Em = 650/675 nm (cut off = 665 nm) with bottom read mode.
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