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Cellular ROS Assay Kit (Red) ab186027 uses a ROS sensor to quantify ROS (Reactive Oxygen Species) in live cells.

Cellular ROS Assay Kit (Red) designed for success:
- Accurate and fast assay with one-step fluorimetric assay to detect intracellular ROS in live cells with 1-2 hours incubation
- Can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation
- Cited in >50 publications

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Images

Functional Studies - Cellular ROS Assay Kit (Red) (AB186027), expandable thumbnail
  • Functional Studies - Cellular ROS Assay Kit (Red) (AB186027), expandable thumbnail

Publications

Key facts

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based (quantitative)

Assay time

60m

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What's included?

200 Test
Components
Assay Buffer
1 x 20 mL
DMSO
1 x 200 µL
ROS Red Dye (Lyophilized)
1 x 1 Vial

Recommended products

Cellular ROS Assay Kit (Red) ab186027 uses a ROS sensor to quantify ROS (Reactive Oxygen Species) in live cells.

Cellular ROS Assay Kit (Red) designed for success:
- Accurate and fast assay with one-step fluorimetric assay to detect intracellular ROS in live cells with 1-2 hours incubation
- Can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation
- Cited in >50 publications

Key facts

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based (quantitative)

Assay time

60m

Assay Platform

Microplate reader, Fluorescence microscope

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Cellular ROS Assay Kit (Red) ab186027 uses a ROS sensor to quantify ROS (Reactive Oxygen Species) in live cells. The red dye used in the ROS assay protocol is cell-permeable and generates red fluorescence when it reacts with ROS. The kit is an optimized "mix and read" assay format that is compatible with HTS liquid handling instruments.

ab186027 provides an ultrasensitive fluorometric one-step ROS assay. The ROS assay can be performed in a convenient 96-well or 384-well microtiter-plate format. Its signal can be easily read by a fluorescence microplate reader at Ex/Em = 520/605 nm and can be used to either quantify ROS levels in cells or to screen ROS inhibitors.

Previously called Cellular Reactive Oxygen Species Detection Assay Kit (Red Fluorescence).Reactive oxygen species (ROS) are natural byproducts of the normal metabolism of oxygen and play important roles in cell signaling. However, during oxidative stress-related states, ROS levels can increase dramatically. The accumulation of ROS results in significant damage to cell structures. The role of oxidative stress in cardiovascular disease, diabetes, osteoporosis, stroke, inflammatory diseases, a number of neurodegenerative diseases and cancer has been well established. The ROS measurement will help to determine how oxidative stress modulates varied intracellular pathways.**Related products**Review the , or the full to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.To measure reactive oxygen species within cells, we recommend . Alternative ROS assays are available in orange (Cellular ROS Assay Kit (Orange) ab186028), red (ab186027), and deep red (Cellular ROS Assay Kit (Deep Red) ab186029). DCF ROS/RNS Assay Kit (biofluids, culture supernatant, cell lysates) ab238535 is used to measure ROS in biofluids, culture supernatants and cell lysates. For assays designed to differentiate ROS, superoxides, and reactive nitrogen species: to assay ROS and superoxides use ROS/Superoxide Detection Assay Kit (Cell-based) ab139476; to assay ROS, superoxides, and reactive nitrogen species use Cellular ROS/RNS Assay Kit ab139473; to assay superoxides use Mitochondrial Superoxide Assay Kit (Fluorometric) ab219943.

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2 product images

  • Functional Studies - Cellular ROS Assay Kit (Red) (ab186027), expandable thumbnail

    Functional Studies - Cellular ROS Assay Kit (Red) (ab186027)

    Bar graph showing relative fluorescence of Reactive Oxygen Species induced in various quantities of Hela cells at various concentrations of H2O2 after 30 min. exposure time.

  • Functional Studies - Cellular ROS Assay Kit (Red) (ab186027), expandable thumbnail

    Functional Studies - Cellular ROS Assay Kit (Red) (ab186027)

    Bar graph showing relative fluorescence of Reactive Oxygen Species induced in various quantities of Hela cells at various concentrations of H2O2 after 120 min. exposure time.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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