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Cellular Superoxide Detection Assay Kit (ab139477) is designed to directly monitor real time superoxide production in live cells using fluorescence microscopy and/or flow cytometry.

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Images

Flow Cytometry - Cellular Superoxide Detection Assay Kit (AB139477), expandable thumbnail

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Key facts

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based

What's included?

1 Kit
Components
ROS Inducer (Pyocyanin 1 µmole)
1 x 1 Vial
ROS Inhibitor (N-acetyl-L-cysteine)
2 x 10 mg
Superoxide Detection Reagent (Orange)
1 x 300 nmol
Wash Buffer Salts
1 x 1 Pack

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Cellular Superoxide Detection Assay Kit (ab139477) is designed to directly monitor real time superoxide production in live cells using fluorescence microscopy and/or flow cytometry.

Key facts

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based

Assay Platform

Flow cytometer, Fluorescence microscope

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Storage information

-80°C

Notes

Cellular Superoxide Detection Assay Kit (ab139477) is designed to directly monitor real time superoxide production in live cells using fluorescence microscopy and/or flow cytometry. A major component of the kit, Superoxide Detection Reagent (Orange), is a cell-permeable probe that reacts specifically with superoxide, generating an orange fluorescent product. The kit is not designed to detect reactive peroxide, hydroxyl, peroxynitrite, chlorine or bromine species, as the fluorescent probe included is relatively insensitive to these analytes. Upon staining, the fluorescent product generated can be visualized using a wide-field fluorescence microscope equipped with standard orange (e.g. 550/620 nm) or red (e.g. 650/670 nm) fluorescent cubes, or cytometrically using any flow cytometer equipped with a blue (488 nm) laser.

The Cellular Superoxide Detection Assay kit contains sufficient reagents for at least 200 microscopy assays or 50 flow cytometry assays using live cells (adherent or in suspension).

**Related products**Review the , or the full to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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1 product image

  • Flow Cytometry - Cellular Superoxide Detection Assay Kit (ab139477), expandable thumbnail

    Flow Cytometry - Cellular Superoxide Detection Assay Kit (ab139477)

    Flow cytometric analysis of Jurkat cells.

    Cellular Superoxide Detection Assay Kit (ab139477) Jurkat cells were induced with 100 μM pyocyanin (general ROS inducer, panel A), or 200 μM antimycin A (superoxide inducer, panel B), stained with Superoxide Detection Reagent (Orange) and analyzed using flow cytometry. Untreated cells (tinted profile) were used as a control. Cell debris were ungated. The numbers within the inserts reflect the mean orange fluorescence of the cells treated and control samples.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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