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AB273296

Ceruloplasmin Activity Assay Kit (Colorimetric)

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Ceruloplasmin Activity Assay Kit (Colorimetric) (ab273296) uses an oxidase substrate, which gives an intensely colored product upon oxidation.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

Ceruloplasmin, Cuproxidase ceruloplasmin, Ferroxidase ceruloplasmin, Glutathione peroxidase ceruloplasmin, Glutathione-dependent peroxiredoxin ceruloplasmin, CP

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Functional Studies - Ceruloplasmin Activity Assay Kit (Colorimetric) (AB273296)
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Supplier Data

Functional Studies - Ceruloplasmin Activity Assay Kit (Colorimetric) (AB273296)

Ceruloplasmin Activity Assay.

Kinetic curves obtained from varying sample volumes of human serum. The kinetic slope for a 10 μl sample of an Ammonium Sulfate precipitate of frozen pooled human serum is 0.0189. Serum Ceruloplasmin Activity = 0.0189 (OD/min)/0.0214 (OD/nmol)/0.01ml X 2 = 176 mU/ml.

Functional Studies - Ceruloplasmin Activity Assay Kit (Colorimetric) (AB273296)
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Supplier Data

Functional Studies - Ceruloplasmin Activity Assay Kit (Colorimetric) (AB273296)

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Typical Standard Curve of chemically oxidized substrate. The Standard Curve slope (for the points below 1.0 OD) is 0.0214 OD/nmol.

Key facts

Detection method

Colorimetric

Sample types

Serum

Assay type

Enzyme activity

Results type

Quantitative

Range

5 - 500 mU/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Ceruloplasmin Activity Assay Kit (Colorimetric) (ab273296) utilizes an oxidase substrate, which gives an intensely colored product upon oxidation. The kit is fast, easy to use and suitable for high throughput applications and can measure ceruloplasmin activity in serum in the range between approximately 5 and 500 mU/ml.

Other Notes
This product was previously called K669 Biovision Ceruloplasmin Activity Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab273296-100Test", "assetComponentDetails": [ { "size":"1 x 1 mL", "name":"Ceruloplasmin Substrate", "number":"AB273296-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"Ceruloplasmin Assay Buffer", "number":"AB273296-CMP04", "productcode":"" }, { "size":"1 x 100 µL", "name":"H2O2 Standard", "number":"AB273296-CMP03", "productcode":"" }, { "size":"1 x 100 µL", "name":"Oxidizer I", "number":"AB273296-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"Ammonium Sulfate", "number":"AB273296-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Please refer to protocols
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Product protocols

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Target data

Multifunctional blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane (PubMed : 16150804). Copper ions provide a large number of enzymatic activites. Oxidizes highly toxic ferrous ions to the ferric state for further incorporation onto apo-transferrins, catalyzes Cu(+) oxidation and promotes the oxidation of biogenic amines such as norepinephrin and serotonin (PubMed : 14623105, PubMed : 4643313, PubMed : 5912351). Provides Cu(2+) ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1 (By similarity). Has glutathione peroxidase-like activity, can remove both hydrogen peroxide and lipid hydroperoxide in the presence of thiols (PubMed : 10481051). Also shows NO-oxidase and NO2 synthase activities that determine endocrine NO homeostasis (PubMed : 16906150).
See full target information CP
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Product promise

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