Ceruloplasmin Activity Assay Kit (Colorimetric) (ab273296) uses an oxidase substrate, which gives an intensely colored product upon oxidation.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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Key facts
- Detection method
Colorimetric
- Sample types
Serum
- Assay type
Enzyme activity (quantitative)
- Range
5 - 500 mU/mL
Target data
Function
Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane. Provides Cu(2+) ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1. May also play a role in fetal lung development or pulmonary antioxidant defense (By similarity).
Alternative names
Ceruloplasmin, Ferroxidase, CP
What's included?
Recommended products
Ceruloplasmin Activity Assay Kit (Colorimetric) (ab273296) uses an oxidase substrate, which gives an intensely colored product upon oxidation.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Alternative names
Ceruloplasmin, Ferroxidase, CP
Key facts
- Detection method
Colorimetric
- Sample types
Serum
- Assay type
Enzyme activity (quantitative)
- Range
5 - 500 mU/mL
- Assay Platform
Microplate
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
Please refer to protocols
Notes
Ceruloplasmin Activity Assay Kit (Colorimetric) (ab273296) utilizes an oxidase substrate, which gives an intensely colored product upon oxidation. The kit is fast, easy to use and suitable for high throughput applications and can measure ceruloplasmin activity in serum in the range between approximately 5 and 500 mU/ml.
This product is manufactured by BioVision, an Abcam company and was previously called K669 Ceruloplasmin Activity Colorimetric Assay Kit. K669-100 is the same size as the 100 test size of ab273296.
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2 product images
Functional Studies - Ceruloplasmin Activity Assay Kit (Colorimetric) (ab273296)
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Typical Standard Curve of chemically oxidized substrate. The Standard Curve slope (for the points below 1.0 OD) is 0.0214 OD/nmol.
Functional Studies - Ceruloplasmin Activity Assay Kit (Colorimetric) (ab273296)
Ceruloplasmin Activity Assay.
Kinetic curves obtained from varying sample volumes of human serum. The kinetic slope for a 10 μl sample of an Ammonium Sulfate precipitate of frozen pooled human serum is 0.0189. Serum Ceruloplasmin Activity = 0.0189 (OD/min)/0.0214 (OD/nmol)/0.01ml X 2 = 176 mU/ml.
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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