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A 2 component chemiluminescent substrate for the detection of horseradish peroxidase (HRP) in western blotting.

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Publications

Reactivity data

Application

WB

Reactivity

Reacts

Dilution info

-

Notes

Incubate membrane in chemiluminescent substrate for 1 minute then rinse off.

What's included?

1 Kit
Components
luminol reagent.
1 x 20 mL
reaction buffer.
2 x 20 mL

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A 2 component chemiluminescent substrate for the detection of horseradish peroxidase (HRP) in western blotting.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

A 2 component chemiluminescent substrate for the detection of horseradish peroxidase (HRP) in western blotting.

The Abcam Chemiluminescent HRP Substrate (membrane) is very sensitive and great care must be taken to optimize the individual assay components (antibodies, conjugates, solid phase, etc.) to minimize background reactivity associated with nonspecific immunochemical reactivities

Prepare 1x substrate by mixing 1 ml of the chemiluminescent reagent with 2 ml of the chemiluminescent reaction buffer. Mix well and protect from light. More or less of the 1x substrate can be prepared using the same ratio.

Stability: Stable for a minimum of 18 months when stored at 2-8ºC in the original container and protected from light. The 1x diluted reagent is stable for several hours at room temperature when protected from light.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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