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AB176767

Chloride Channel Assay Kit (Colorimetric)

5

(1 Review)

|

(2 Publications)

Chloride Channel Assay Kit ab176767 provides a sensitive and robust colorimetric method for studying chloride channels.
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Functional Studies - Chloride Channel Assay Kit (Colorimetric) (AB176767)
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Supplier Data

Functional Studies - Chloride Channel Assay Kit (Colorimetric) (AB176767)

Sodium iodide (Nal) dose response was measured with Abcam's Chloride Channel Assay Kit (Colorimetric) (ab176767) in a 96-well black wall/clear bottom plate. As low as 30 nM of Nal was detected with 10 minutes incubation time (n = 3).

Key facts

Detection method

Colorimetric

Sample types

Suspension cells, Adherent cells

Assay type

Quantitative

Assay Platform

Microplate reader

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Product details

Abcam's Chloride Channel Assay Kit (Colorimetric) (ab176767) provides a sensitive and robust colorimetric method for studying chloride channels. The assay is based on our proprietary iodide indicator (Iodide Sensor Blue dye) to measure iodide concentration, as low as 30 nM of iodide can be detected. Iodide Sensor Blue dye forms a blue complex with iodide, which has absorption spanning from the UV to 700 nm. Thus a few absorption wavelengths can be used for monitoring the iodide-dependent color change.

Abcam's Chloride Channel Assay Kit (Colorimetric) (ab176767) provides an optimized assay method for monitoring chloride channels. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format.

Chloride channels have a variety of important physiological and cellular functions that include regulation of pH, volume homeostasis, organic solute transport, cell migration, cell proliferation and differentiation. Chloride channels represent valuable drug targets. A number of chronic disease states such as cystic fibrosis and Bartter's syndrome are due to defects in chloride channel functions. However, the existing technologies for screening chloride channel modulators are a compromise between throughput, sensitivity and physiological relevance.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab176767-10x96Tests", "assetComponentDetails": [ { "size":"1 x 5 mL", "name":"10X Cell Lysis Buffer", "number":"AB176767-CMP04", "productcode":"" }, { "size":"1 x 50 mL", "name":"Iodide Sensor Blue dye", "number":"AB176767-CMP02", "productcode":"" }, { "size":"1 x 100 mL", "name":"I-Loading Buffer", "number":"AB176767-CMP01", "productcode":"" }, { "size":"1 x 0.5 mL", "name":"100X Iodide Sensor Enhancer", "number":"AB176767-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Chloride channels also known as Cl⁻ channels are integral membrane proteins which mediate the transport of chloride ions across cell membranes. These channels vary in function and structure but they generally allow the passive flow of Cl⁻ ions contributing to the electrochemical gradient maintenance. Chloride channels often have a molecular mass ranging from 70 kDa to 200 kDa depending on the type and species. They express widely including in neurons epithelial cells and muscle tissues reflecting their involvement in numerous physiological processes. Notably some chloride channels play critical roles in sensory pathways in the eye and ear.
Biological function summary

Chloride channels facilitate the regulation of cell volume neuronal excitability and epithelial fluid transport. They associate with various protein complexes such as those involving GABA and glycine receptors where they influence synaptic inhibition. Additionally several subtypes like CLC-0 CLC-1 and CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) participate directly in cellular signaling pathways. They function as key determinants of pH and ion homeostasis within cells and organelles.

Pathways

Chloride channels integrate into multiple major biological processes. Among these the GABAergic and glycinergic synaptic transmission pathways are prominent where chloride channels modulate inhibitory neurotransmission. Proteins like the GABA(A) receptor and glycine receptor closely interact with chloride channels further influencing inhibition at synaptic junctions. Furthermore in the kidney's nephron chloride channels like the CLC-K family regulate electrolyte balance illustrating their importance in renal function pathways.

Chloride channels link several health conditions prominently cystic fibrosis and myotonia congenita. Cystic fibrosis arises from mutations in the CFTR gene which codes for a chloride channel essential for fluid transport in epithelial tissues. This connection highlights the channel's role in disease pathophysiology. Moreover chloride channel dysfunctions particularly in the CLC family contribute to myotonia congenita a disorder characterized by muscle stiffness. This condition highlights the channel's importance in neuromuscular pathways and its association with proteins like the CLC-1 channel framed within the context of skeletal muscle activity.
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Product protocols

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Target data

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Circulation research 124:52-65 PubMed30582444

2018

CLIC4/Arf6 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Vahitha B Abdul-Salam,Giusy Russomanno,Chen Chien-Nien,Abdul S Mahomed,Luke A Yates,Martin R Wilkins,Lan Zhao,Magdalena Gierula,Oliver Dubois,Ute Schaeper,Jens Endruschat,Beata Wojciak-Stothard

Digestive and liver disease : official journal of 47:964-72 PubMed26277684

2015

Development and functional characterization of extrahepatic cholangiocyte lines from normal rats.

Applications

Unspecified application

Species

Unspecified reactive species

Julie Venter,Heather Francis,Fanyin Meng,Sharon DeMorrow,Lindsey Kennedy,Holly Standeford,Laura Hargrove,Nan Wu,Ying Wan,Gabriel Frampton,Matthew McMillin,Marco Marzioni,Eugenio Gaudio,Paolo Onori,Shannon Glaser,Gianfranco Alpini
View all publications
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