Cholesterol Assay Kit - HDL and LDL/VLDL

• Biochemical assay

Lab

Biochemical assay - Cholesterol Assay Kit - HDL and LDL/VLDL (AB65390)

Diagram showing the principles of the Cholesterol assay method.

• FuncS

PubMed

Functional Studies - Cholesterol Assay Kit - HDL and LDL/VLDL (AB65390)

Wild type (WT) or farnesoid X receptor knock-out (FRX-KO) mice were treated with either vehicle (Veh) or a high fat-containing diet (HFD) for 16 weeks. Serum HDL (top) and LDL/VLDL cholesterol (bottom) from 5-7 mice/group was measured using ab65390 following protocol instructions. An asterisk (*) means P<0.05 between WT and FXR-KO vehicle group. A pound (#) indicates P<0.05 between Wt and FXR-KO-HFD group. Image obtained from Li G et al., PLoS One, 2012; 7(4) : e35895

Li G et al., PLoS One, 7, e35895, 2012 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• FuncS

Lab

Functional Studies - Cholesterol Assay Kit - HDL and LDL/VLDL (AB65390)

Rat serum samples were processed according to the protocol. The quantity of total (dilution range 1 : 10-1 : 100), HDL (dilution range 1 : 1-1 : 10), LDL/VLDL (dilution range 1 : 1-1 : 10) and free cholesterol (neat) was measured colourimetrically, in duplicates, after 40 minutes.

• FuncS

Supplier Data

Functional Studies - Cholesterol Assay Kit - HDL and LDL/VLDL (AB65390)

Measurement of total cholesterol, HDL, LDL/VLDL from serum samples. Total Cholesterol (blue), HDL (green), and LDL/VLDL (cream) cholesterol were measured following the kit protocol.

• FuncS

Lab

Functional Studies - Cholesterol Assay Kit - HDL and LDL/VLDL (AB65390)

Signal from standard curve was measured colourimetrically over a period of time. Background signal subtracted and each point on the curve represents duplicate values (+/- SD).

• Biochemical assay

PubMed

Biochemical assay - Cholesterol Assay Kit - HDL and LDL/VLDL (AB65390)

Triglyceride assay kit ab65336 and cholesterol assay kit ab65390 used with mouse serum.

Zuo et al. used Triglyceride assay kit ab65336 and Cholesterol assay kit ab65390 to investigate the impact of hepatocyte-specific AAV-mediated knockout of ANGPTL3 in mice.

Measurements of serum triglyceride (TG) and total cholesterol (TC) in mice at 1–4 weeks after AAV9-hAAT-Anc/Angptl3 administration. The control (Ctrl) mice received no treatments.

Blood samples collected from the animals were allowed for clotting at room temperature for 10 min. Serum was isolated by centrifugation at 5000 rpm for 10 min and stored at -80 °C in small aliquots. Determination of TG and TC was performed using the triglyceride and cholesterol assay kit (Abcam, ab65336 and ab65390, respectively) according to the manufacture’s instruction.

• Biochemical assay

PubMed

Biochemical assay - Cholesterol Assay Kit - HDL and LDL/VLDL (AB65390)

Cholesterol Assay Kit - HDL and LDL/VLDL ab65390 used with mouse serum.

Lee et al. used Cholesterol Assay Kit - HDL and LDL/VLDL ab65390 to investigate how thyme extract might mitigate high fat diet induced metabolic derangements and improve the gut environment.

Effects of thyme leaf extract (TLE) on serum indicators of liver function, lipid parameters, and blood glucose tolerance level in high-fat diet (HFD)-induced obese mice : Serum total cholesterol; serum HDL cholesterol; LDL cholesterol. ND, normal diet; HFD, high fat diet; PC, fructooligosaccharides fed + high fat; TLE50 and TLE100, thyme leaf extract high fat.

After a 12 h fasting period, mice were humanely euthanized by isoflurane exposure, and blood samples were collected. Serum was isolated by centrifuging the blood at 1500Ч g for 15 min and was stored at 80 °C. Biochemical analyses of the serum and liver samples were conducted using commercial ELISA kits : Abcam; Cholesterol, ab65390, Cambridge, UK.

• Biochemical assay

PubMed

Biochemical assay - Cholesterol Assay Kit - HDL and LDL/VLDL (AB65390)

Triglyceride assay kit ab65336, Cholesterol assay kit ab65390, and Free Fatty Acid assay kit ab65341 used with rat serum and rat liver.

Li et al. used Triglyceride assay kit ab65336, Cholesterol assay kit ab65359, and Free Fatty Acid assay kit ab65341 to investigate the impact of using L. reuteri treatment to ameliorate the impact of biorhythm disorder-ignited dyslipidemia in long-term darkness treated rats.

The concentrations of triglycerides (TG), total cholesterol (CHOL), HDL-cholesterol (HDL-C), and free fatty acids (NEFA) in rat sera and liver tissues were detected using Triglyceride Quantification Assay Kit (ab65336), HDL and LDL/VLDL Cholesterol Assay Kit (ab65390). and Free Fatty Acid Quantification Assay Kit (ab65341), respectively. All procedures were carried out following the recommended instructions provided by the kit manufacturers.

• Schematic Diagram

Supplier Data

Schematic Diagram - Cholesterol Assay Kit - HDL and LDL/VLDL (AB65390)

Representative image of Cholesterol Assay Kit - HDL and LDL/VLDL ab65390

Components shown from left to right :

- OxiRed Probe

- Cholesterol Esterase

- Cholesterol Standard

- Enzyme Mix I

- Assay Buffer 2

- 2X LDL/VLDL Precipitation Buffer

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.