Skip to main content

Cholesterol Assay Kit ab65390 is used to quantify total cholesterol, free cholesterol, and cholesterol esters, and includes a method to separate HDL and LDL/VLDL fractions. The assay has one 60 min incubation, with colorimetric (570 nm) or fluorometric (535/587 nm) readout.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

Detection method

Colorimetric/Fluorometric

Sample types

Tissue Lysate, Urine, Plasma, Serum, Other biological fluids

Assay type

Quantitative

Assay time

1h

Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...

Images

What's included?

100 Test
Components
2X LDL/VLDL Precipitation Buffer
1 x 10 mL
Assay Buffer II
1 x 25 mL
Cholesterol Esterase
1 x 1 Vial
Cholesterol Standard
1 x 100 µL
Enzyme Mix I
1 x 1 Vial
OxiRed Probe
1 x 200 µL

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Cholesterol Assay Kit ab65390 is used to quantify total cholesterol, free cholesterol, and cholesterol esters, and includes a method to separate HDL and LDL/VLDL fractions. The assay has one 60 min incubation, with colorimetric (570 nm) or fluorometric (535/587 nm) readout.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Tissue Lysate, Urine, Plasma, Serum, Other biological fluids

Assay type

Quantitative

Assay time

1h

Assay Platform

Microplate reader

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Cholesterol Assay Kit ab65390 uses a simple method to quantify total cholesterol, free cholesterol, and cholesterol esters in mammalian samples. It also includes an easy method to separate HDL and LDL / VLDL cholesterol.

In the cholesterol assay protocol, cholesterol oxidase acts on free cholesterol to produce a chemical which reacts with a probe to generate color (570 nm) and fluorescence (Ex/Em = 538/587 nm). Cholesterol esterase is used to hydrolyze cholesteryl ester into free cholesterol.

If cholesterol esterase is included in the assay, total cholesterol is measured. If it is not included, free cholesterol is measured. The amount of cholesterol ester can be calculated by subtracting free cholesterol from total cholesterol.

Note: this kit is primarily designed for use with serum and plasma samples. It has been successfully used with liver samples (including in Chang et al. 2019, PMID: 30670059, Yu et al. 2018, PMID: 29867579 and Sadh et al. 2017, PMID: 28800633); sample preparation in liver (and other tissue) samples may need optimization and modification by the user for successful use.

Cholesterol assay protocol summary:
- use complete sample for cholesterol measurement; or to separate HDL and LDL/VLDL, mix sample with precipitation buffer, incubate for 10 min, centrifuge for 10 min at 2,000 g, keep HDL fraction supernatant, repeat centrifugation and resuspend LDL/VLDL fraction pellet
- for assay, add samples and standards to wells
- add total cholesterol reaction mix (with esterase) or free cholesterol reaction mix (without esterase) and incubate for 60 min at 37°C
- analyze with microplate reader

Other cholesterol assay kits include: - - - - Review our to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader. **How other researchers have used Cholesterol Assay Kit ab65390** This cholesterol assay kit has been used in publications in a variety of sample types, including: - Human: serum1 - Mouse: plasma and liver tissue2, plasma3, serum4,1 - Rat: liver tissue5, serum1, plasma6 - Fish: *Z*. *temminckii* serum*7* References: 1 - Lin C et al 2018; 2 - Change RC et al 2019; 3 - Hinder et al 2019, Havighorst A et al 2019, Guo X et al 2017, Wen et al 2018, Fernandes GW et al 2018, Ahnstedt H et al 2018, Dumas SN and Ntambi JM 2018; 4 - Liu J et al 2018, Cui XB et al 2019, Rohm et al 2018; 5 - Yu S et al 2018; 6 - Park JB 2017; 7 - Chae Y et al 2018

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com