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AB102515

Cholesterol/Cholesteryl Ester Assay Kit - Detection

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(20 Publications)

Cholesterol/Cholesteryl Ester Assay Kit ab102515 is used to quantify free cholesterol, cholesteryl esters, or both together. Readout using any colorimetric (450 nm) plate reader.
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Functional Studies - Cholesterol/Cholesteryl Ester Assay Kit - Detection (AB102515)
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Supplier Data

Functional Studies - Cholesterol/Cholesteryl Ester Assay Kit - Detection (AB102515)

Cholesterol Standard Curve : Cholesterol/Cholesteryl Ester was quantified according to the kit instructions. Background from the 0 standard reading (without cholesterol) has been subtracted from all readings.

Key facts

Detection method

Colorimetric

Sample types

Tissue Lysate, Cell Lysate

Assay type

Quantitative

Assay time

1h

Assay Platform

Microplate reader

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Product details

Cholesterol/Cholesteryl Ester Assay Kit ab102515 uses a simple method for sensitive quantification of free cholesterol, cholesteryl esters, or both using a colorimetric method.

This assay kit uses an alternative method compared to our most popular Cholesterol/ Cholesteryl Ester Assay Kit ab65359 (which relies on a cholesterol oxidase based method).

Cholesterol assay principle
In this cholesterol assay, free cholesterol is oxidized by cholesterol dehydrogenase to generate NADH which reacts with a sensitive probe resulting in strong absorbance at 450 nm.

The assay can detect free or total cholesterol depending upon whether cholesterol esterase is used to hydrolyze cholesterol esters present.

Cholesteryl ester can be determined by subtracting the value of free cholesterol from the total cholesterol (cholesterol plus cholesteryl esters).

Cholesteryl assay protocol summary
- add samples and standard to wells
- add reaction mix and incubate for 30-min at 37°C
- analyse with plate reader

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab102515-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Cholesterol Enzyme Mix", "number":"AB102515-CMP03", "productcode":"" }, { "size":"1 x 100 µL", "name":"Cholesterol Standard", "number":"AB102515-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Cholesterol Esterase", "number":"AB102515-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 2", "number":"AB102515-CMP01", "productcode":"" }, { "size":"1 x 1 Each", "name":"Developer Solution III", "number":"AB102515-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cholesterol/cholesteryl ester (CE) plays a mechanical role in the body by transporting lipids. Known also as 'cholesteryl esters' these molecules consist of cholesterol bound to long-chain fatty acids. CE exhibits a molecular mass that varies depending on the fatty acid chain length. These molecules are typically found within plasma and intracellular lipid droplets in tissues such as the liver and intestines. They mediate cholesterol transport and storage maintaining cellular lipid homeostasis.
Biological function summary

Cholesteryl esters facilitate cholesterol influx and efflux by serving as precursor molecules for various hormones and vitamin D. They do not exist in isolation but rather as part of larger lipid complexes including chylomicrons very-low-density lipoproteins (VLDL) and low-density lipoproteins (LDL). These lipid complexes play an essential role in delivering cholesterol to peripheral tissues and returning excess to the liver for excretion or reprocessing.

Pathways

Cholesteryl esters are involved in lipid metabolism processes such as the reverse cholesterol transport and the esterification of cholesterol through the action of lecithin-cholesterol acyltransferase (LCAT). This esterification process converts free cholesterol into cholesteryl esters facilitating their transport within lipoproteins. High-density lipoprotein (HDL) serves as a pathway partner circulating cholesteryl esters back to the liver where they undergo hydrolysis and re-utilization.

Cholesteryl esters are significant in the development of atherosclerosis and coronary artery disease. Dysregulation of CE metabolism can lead to the accumulation of lipid-rich plaques in blood vessel walls catalyzed by proteins like apolipoproteins such as ApoB-100 and ApoE. Excessive LDL and CE deposition in arterial walls are associated with plaque formation culminating in cardiovascular diseases. Understanding the role of these compounds and their associated pathways is important for developing treatments against lipid disorders.
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Product protocols

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Target data

See full target information Cholesterol
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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed36835419

2023

ERRα Up-Regulates Invadopodia Formation by Targeting HMGCS1 to Promote Endometrial Cancer Invasion and Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Shuting Tang,Jincheng Ma,Pingping Su,Huifang Lei,Yao Tong,Liangzhi Cai,Shuxia Xu,Xiaodan Mao,Pengming Sun

Frontiers in genetics 13:931017 PubMed35957687

2022

Re-Expression of Tafazzin Isoforms in TAZ-Deficient C6 Glioma Cells Restores Cardiolipin Composition but Not Proliferation Rate and Alterations in Gene Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Gayatri Jagirdar,Matthias Elsner,Christian Scharf,Stefan Simm,Katrin Borucki,Daniela Peter,Michael Lalk,Karen Methling,Michael Linnebacher,Mathias Krohn,Carmen Wolke,Uwe Lendeckel

Cell death & disease 13:488 PubMed35597782

2022

FASN promotes lymph node metastasis in cervical cancer via cholesterol reprogramming and lymphangiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Qiqiao Du,Pan Liu,Chunyu Zhang,Tianyu Liu,Wei Wang,Chunliang Shang,Jieyu Wu,Yuandong Liao,Yili Chen,Jiaming Huang,Hao Tan,Yunhe Zhao,Meng Xia,Junxiu Liu,Shuzhong Yao

International journal of molecular sciences 22: PubMed34360754

2021

Knockdown of PEX16 Induces Autophagic Degradation of Peroxisomes.

Applications

Unspecified application

Species

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Xiaofan Wei,Yunash Maharjan,Debra Dorotea,Raghbendra-Kumar Dutta,Donghyun Kim,Hyunsoo Kim,Yizhu Mu,Channy Park,Raekil Park

JCI insight 6: PubMed34236046

2021

Zebrafish mutants provide insights into Apolipoprotein B functions during embryonic development and pathological conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Hanoch Templehof,Noga Moshe,Inbal Avraham-Davidi,Karina Yaniv

Cancer genomics & proteomics 18:385-405 PubMed33994363

2021

Metabolic Response to the Mitochondrial Toxin 1-Methyl-4-phenylpyridinium (MPP+) in LDH-A/B Double-knockout LS174T Colon Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Nzinga Mack,Elizabeth Mazzio,Ramesh Badisa,Karam F A Soliman

Cell metabolism 33:939-956.e8 PubMed33770509

2021

Placental superoxide dismutase 3 mediates benefits of maternal exercise on offspring health.

Applications

Unspecified application

Species

Unspecified reactive species

Joji Kusuyama,Ana Barbara Alves-Wagner,Royce H Conlin,Nathan S Makarewicz,Brent G Albertson,Noah B Prince,Shio Kobayashi,Chisayo Kozuka,Magnus Møller,Mette Bjerre,Jens Fuglsang,Emily Miele,Roeland J W Middelbeek,Yang Xiudong,Yang Xia,Léa Garneau,Jayonta Bhattacharjee,Céline Aguer,Mary Elizabeth Patti,Michael F Hirshman,Niels Jessen,Toshihisa Hatta,Per Glud Ovesen,Kristi B Adamo,Eva Nozik-Grayck,Laurie J Goodyear

EMBO reports 21:e48901 PubMed32157776

2020

TMEM135 regulates primary ciliogenesis through modulation of intracellular cholesterol distribution.

Applications

Unspecified application

Species

Unspecified reactive species

Yunash Maharjan,Joon No Lee,Seong Ae Kwak,Raghbendra Kumar Dutta,Channy Park,Seong-Kyu Choe,Raekil Park

Theranostics 9:6157-6174 PubMed31534543

2019

CAV1 - GLUT3 signaling is important for cellular energy and can be targeted by Atorvastatin in Non-Small Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Azhar Ali,Elena Levantini,Chee Wai Fhu,Jun Ting Teo,John G Clohessy,Julian L Goggi,Chan-Shuo Wu,Leilei Chen,Tan Min Chin,Daniel G Tenen

Cell 177:722-736.e22 PubMed30955890

2019

Insulin Receptor Associates with Promoters Genome-wide and Regulates Gene Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Melissa L Hancock,Rebecca C Meyer,Meeta Mistry,Radhika S Khetani,Alexandre Wagschal,Taehwan Shin,Shannan J Ho Sui,Anders M Näär,John G Flanagan
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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