Cholesterol Uptake Assay Kit

• FuncS

Supplier Data

Functional Studies - Cholesterol Uptake Assay Kit (AB236212)

U-18666A causes a dose-dependent increase in NBD Cholesterol uptake in Caco-2 cells, as measured on a fluorescent plate reader

• FuncS

Supplier Data

Functional Studies - Cholesterol Uptake Assay Kit (AB236212)

U-18666A increases cholesterol uptake in Jurkat cells as measured by flow cytometry.

Jurkat cells were seeded at a density of 5x10⁵ cells/mL and incubated overnight in serum-free RPMI with U-18666A or vehicle and 20 μg/mL NBD Cholesterol in a cell culture incubator at 37°C. The next day, cells were transferred to a v-bottom plate for washing and reading on a flow cytometer. Cholesterol uptake was evaluated in the live cell gate using FlowJo analysis software. U-18666A at both 2.5 μM (green) and 1.25 μM (blue) showed a significant (p<0.05, t-test) shift in mean fluorescence as compared to the vehicle control (black) and the untreated (red) cells.

• FuncS

Supplier Data

Functional Studies - Cholesterol Uptake Assay Kit (AB236212)

Blocking intracellular cholesterol transport with U-18666A increases NBD cholesterol uptake