Choline/Acetylcholine Assay Kit ab65345 provides a simple and sensitive means for quantifying Choline and Acetylcholine by either a colorimetric or fluorometric method in samples such as blood, cells, culture media, fermentation media, etc.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Cell research 34:245-2572024MFSD7c functions as a transporter of choline at the blood-brain barrier.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuan Thi Anh Nguyen,Thanh Nha Uyen Le,Toan Q Nguyen,Hoa Thi Thuy Ha,Anna Artati,Nancy C P Leong,Dat T Nguyen,Pei Yen Lim,Adelia Vicanatalita Susanto,Qianhui Huang,Ling Fam,Lo Ngah Leong,Isabelle Bonne,Angela Lee,Jorge L Granadillo,Catherine Gooch,Dejie Yu,Hua Huang,Tuck Wah Soong,Matthew Wook Chang,Markus R Wenk,Jerzy Adamski,Amaury Cazenave-Gassiot,Long N NguyenPubMed 38302740
- Heliyon 9:e174342023Non-neuronal cholinergic system delays cardiac remodelling in type 1 diabetes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPujika Emani Munasinghe,Eng Leng Saw,Matthew Reily-Bell,Devin Tonkin,Yoshihiko Kakinuma,Martin Fronius,Rajesh KatarePubMed 37426799
- iScience 26:1069542023SARS-CoV-2 S1 protein causes brain inflammation by reducing intracerebral acetylcholine production.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNaomi Oka,Kazuya Shimada,Azusa Ishii,Nobuyuki Kobayashi,Kazuhiro KondoPubMed 37275532
- International journal of molecular sciences 24:2023Auditory or Audiovisual Stimulation Ameliorates Cognitive Impairment and Neuropathology in ApoE4 Knock-In Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHarry Jung,Yeonkyeong Lee,Sang-Hwa Lee,Jong-Hee SohnPubMed 36674449
- Scientific reports 12:209032022Acetylcholine deficit causes dysfunctional inhibitory control in an aging-dependent manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPaul Rafael Sabandal,Erick Benjamin Saldes,Kyung-An HanPubMed 36463374
- Cells 11:2022Impact of Neurons on Patient-Derived Cardiomyocytes Using Organ-On-A-Chip and iPSC Biotechnologies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlbin A Bernardin,Sarah Colombani,Antoine Rousselot,Virginie Andry,Yannick Goumon,Hélène Delanoë-Ayari,Côme Pasqualin,Bernard Brugg,Etienne D Jacotot,Jean-Luc Pasquié,Alain Lacampagne,Albano C MeliPubMed 36497024
- Cell reports 40:1113082022Enterobacter ludwigii protects DSS-induced colitis through choline-mediated immune tolerance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQianqian Li,Xuan Sun,Kaiyuan Yu,Junqiang Lv,Chunhui Miao,Jianming Yang,Song Wang,Zheng Fu,Yamin Sun,Hong Zhang,Zhi-Song Zhang,Evan T Keller,Zhi Yao,Quan WangPubMed 36044853
- Mucosal immunology 15:1283-12952022Microbial regulation of intestinal motility provides resistance against helminth infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMati Moyat,Luc Lebon,Olaf Perdijk,Lakshanie C Wickramasinghe,Mario M Zaiss,Ilaria Mosconi,Beatrice Volpe,Nadine Guenat,Kathleen Shah,Gillian Coakley,Tiffany Bouchery,Nicola L HarrisPubMed 35288644
- Cell stem cell 29:528-544.e92022A cholinergic neuroskeletal interface promotes bone formation during postnatal growth and exercise.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStephen Gadomski,Claire Fielding,Andrés García-García,Claudia Korn,Chrysa Kapeni,Sadaf Ashraf,Javier Villadiego,Raquel Del Toro,Olivia Domingues,Jeremy N Skepper,Tatiana Michel,Jacques Zimmer,Regine Sendtner,Scott Dillon,Kenneth E S Poole,Gill Holdsworth,Michael Sendtner,Juan J Toledo-Aral,Cosimo De Bari,Andrew W McCaskie,Pamela G Robey,Simón Méndez-FerrerPubMed 35276096
- Nature communications 13:5432022Cholinergic signals preserve haematopoietic stem cell quiescence during regenerative haematopoiesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesClaire Fielding,Andrés García-García,Claudia Korn,Stephen Gadomski,Zijian Fang,Juan L Reguera,José A Pérez-Simón,Berthold Göttgens,Simón Méndez-FerrerPubMed 35087060
Key facts
- Detection method
Colorimetric/Fluorometric
- Sample types
Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids
- Assay type
Quantitative
- Reactive species
Mammals
- Range
0.01 - 5 nmol/well
- Assay time
40m
- Sensitivity
> 0.01 nmol/well
Target data
What's included?
Recommended products
Choline/Acetylcholine Assay Kit ab65345 provides a simple and sensitive means for quantifying Choline and Acetylcholine by either a colorimetric or fluorometric method in samples such as blood, cells, culture media, fermentation media, etc.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
Colorimetric/Fluorometric
- Sample types
Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids
- Assay type
Quantitative
- Reactive species
Mammals
- Range
0.01 - 5 nmol/well
- Assay time
40m
- Assay Platform
Microplate reader
- Sensitivity
> 0.01 nmol/well
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Choline/Acetylcholine Assay Kit (ab65345) provides a simple and sensitive means for quantifying Choline and Acetylcholine by either a colorimetric or fluorometric method in samples such as blood, cells, culture media, fermentation media, etc.
In the choline / acetylcholine assay protocol, free choline is oxidized to betaine, via the intermediate betaine aldehyde. The reaction generates products which react with the Choline Probe to generate color (λ= 570 nm), and fluorescence (Ex/Em 535/587 nm).
Acetylcholine can be converted to choline by adding acetylcholinesterase to the reaction to measure total choline (choline + acetyl choline). The amount of acetyl choline is calculated by subtracting choline from total choline.
There is no need for pretreatment or purification of samples. The kit can detect ~10 pmol-5 nmol of choline or acetylcholine.
Cells should be cultured in choline chloride free medium.
Choline / acetylcholine assay protocol summary:
- add samples and standards to wells
- add choline reaction mix with, or without, acetylcholinesterase, and incubate for 30 min at room temp
- analyze with microplate reader
If you need to purchase additional Choline Probe for this product, please see .
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Choline also known by its alternate name trimethylammonium ethanol is a water-soluble essential nutrient. It serves as a precursor for the neurotransmitter acetylcholine which is critical for numerous neural functions. Acetylcholine a small molecule with a molecular mass of 146.21 g/mol is synthesized in nerve terminals from choline and acetyl-CoA by the enzyme choline acetyltransferase. This target is widely expressed across both the central and peripheral nervous systems and its availability and uptake in nerve cells are important for maintaining optimal levels of acetylcholine.
- Biological function summary
Choline and its derivative acetylcholine play important roles in cell membrane integrity and neurotransmission. Acetylcholine a constituent of the cholinergic system functions as a neurotransmitter involved in muscle activation cognitive processes and modulation of arousal. It does not typically form part of a larger complex but works in synaptic clefts to transmit signals between neurons. The presence of acetylcholine depends on the availability of choline the substrate used for its synthesis.
- Pathways
Choline and acetylcholine are integral to the cholinergic signaling pathway. Acetylcholine interacts with muscarinic and nicotinic receptors to transmit neural signals. These receptors are vital parts of the pathway that modulates parasympathetic nervous system responses. Choline kinase involved in phosphorylating choline to phosphocholine links the choline metabolism pathway to cell signaling and fatty acid biosynthesis.
- Associated diseases and disorders
Choline and acetylcholine are associated with conditions like Alzheimer's disease and myasthenia gravis. Alzheimer's disease involves decreased levels of acetylcholine due to cholinergic neuron degeneration impacting cognitive functions. In myasthenia gravis an autoimmune disorder antibodies target acetylcholine receptors leading to muscle weakness. Both conditions highlight the importance of acetylcholine's relation to choline acetyltransferase as this enzyme's activity is pivotal for maintaining acetylcholine levels essential for normal nervous system function.
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5 product images
Image from Kim J.H et al., PLoS One 8(2), Fig 4. doi: 10.1371/journal.pone.0056736. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Functional Studies - Choline/Acetylcholine Assay Kit (ab65345)
Acetylcholine levels from mouse brain tissue was measured using Acetylcholine assay kit (ab65345). Normal mice were anesthetized with thiopental sodium at 20minutes after the end of electroacupuncture (EA) treatment to determine levels of ACh (Figure A). Immunohistochemical staining mAChR (muscarinic acetylcholine receptor) M3 in mouse cerebral cortex tissue 20 minutes after the end of EA treatment (Image B).
Functional Studies - Choline/Acetylcholine Assay Kit (ab65345)
Functional Studies - Choline/Acetylcholine Assay Kit (ab65345)
Choline measured fluorometrically in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample
Functional Studies - Choline/Acetylcholine Assay Kit (ab65345)
Choline measured fluorometrically in cell lysates showing quantity (pmol) per 1 mln of tested cells
Functional Studies - Choline/Acetylcholine Assay Kit (ab65345)
Choline measured fluorometrically in biological fluids showing quantity (nmol) per mL of tested sample
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