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AB65345

Choline/Acetylcholine Assay Kit

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(70 Publications)

Choline/Acetylcholine Assay Kit ab65345 provides a simple and sensitive means for quantifying Choline and Acetylcholine by either a colorimetric (570 nm) or fluorometric (Ex/Em 535/587 nm) method in samples such as blood, cells, culture media, fermentation media.

- Cited in >60 publications
- Kit includes standard curve for quantitation
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
6 Images
Functional Studies - Choline/Acetylcholine Assay Kit (AB65345)
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Lab

Functional Studies - Choline/Acetylcholine Assay Kit (AB65345)

Choline measured fluorometrically in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample

Functional Studies - Choline/Acetylcholine Assay Kit (AB65345)
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PubMed

Functional Studies - Choline/Acetylcholine Assay Kit (AB65345)

Acetylcholine levels from mouse brain tissue was measured using Acetylcholine assay kit (ab65345). Normal mice were anesthetized with thiopental sodium at 20minutes after the end of electroacupuncture (EA) treatment to determine levels of ACh (Figure A). Immunohistochemical staining mAChR (muscarinic acetylcholine receptor) M3 in mouse cerebral cortex tissue 20 minutes after the end of EA treatment (Image B).

Image from Kim J.H et al., PLoS One 8(2), Fig 4. doi: 10.1371/journal.pone.0056736. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Functional Studies - Choline/Acetylcholine Assay Kit (AB65345)
  • FuncS

Lab

Functional Studies - Choline/Acetylcholine Assay Kit (AB65345)

Choline measured fluorometrically in biological fluids showing quantity (nmol) per mL of tested sample

Functional Studies - Choline/Acetylcholine Assay Kit (AB65345)
  • FuncS

Lab

Functional Studies - Choline/Acetylcholine Assay Kit (AB65345)
Functional Studies - Choline/Acetylcholine Assay Kit (AB65345)
  • FuncS

Lab

Functional Studies - Choline/Acetylcholine Assay Kit (AB65345)

Choline measured fluorometrically in cell lysates showing quantity (pmol) per 1 mln of tested cells

Other - Choline/Acetylcholine Assay Kit (AB65345)
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Supplier Data

Other - Choline/Acetylcholine Assay Kit (AB65345)

Representative image of Choline/Acetylcholine Assay Kit ab65345

Components shown from left to right :

- OxiRed Probe

- Acetycholinesterase Enzyme

- Choline Standard

- Enzyme Mix IV

- Assay Buffer 6

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids

Results type

Quantitative

Sensitivity

> 0.01 nmol/well

Range

0.01 - 5 nmol/well

Assay time

40m

Assay Platform

Microplate reader

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Primary Antibodies

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KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

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Product details

Choline/Acetylcholine Assay Kit (ab65345) provides a simple and sensitive means for quantifying Choline and Acetylcholine by either a colorimetric or fluorometric method in samples such as blood, cells, culture media, fermentation media, etc.

In the choline / acetylcholine assay protocol, free choline is oxidized to betaine, via the intermediate betaine aldehyde. The reaction generates products which react with the Choline Probe to generate color (λ= 570 nm), and fluorescence (Ex/Em 535/587 nm).

Acetylcholine can be converted to choline by adding acetylcholinesterase to the reaction to measure total choline (choline + acetyl choline). The amount of acetyl choline is calculated by subtracting choline from total choline.

There is no need for pretreatment or purification of samples. The kit can detect ~10 pmol-5 nmol of choline or acetylcholine.

Cells should be cultured in choline chloride free medium.

Choline / acetylcholine assay protocol summary:
- add samples and standards to wells
- add choline reaction mix with, or without, acetylcholinesterase, and incubate for 30 min at room temp
- analyze with microplate reader

If you need to purchase additional Choline Probe for this product, please see .

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "2000Test": { "sellingSize": "2000 Test", "publicAssetCode":"ab65345-2000Test", "assetComponentDetails": [ { "size":"20 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB65345-CMP04" }, { "size":"20 x 1 Vial", "name":"Enzyme Mix IV", "number":"AB65345-CMP03" }, { "size":"20 x 1 Vial", "name":"Choline Standard", "number":"AB65345-CMP05" }, { "size":"20 x 25 mL", "name":"Assay Buffer 6", "number":"AB65345-CMP02" }, { "size":"20 x 1 Vial", "name":"Acetylcholinesterase Enzyme", "number":"AB65345-CMP01" } ] }, "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab65345-100Test", "assetComponentDetails": [ { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB65345-CMP04", "productcode":"AB309437" }, { "size":"1 x 1 Vial", "name":"Enzyme Mix IV", "number":"AB65345-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Choline Standard", "number":"AB65345-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 6", "number":"AB65345-CMP02", "productcode":"AB323964" }, { "size":"1 x 1 Vial", "name":"Acetylcholinesterase Enzyme", "number":"AB65345-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Choline also known by its alternate name trimethylammonium ethanol is a water-soluble essential nutrient. It serves as a precursor for the neurotransmitter acetylcholine which is critical for numerous neural functions. Acetylcholine a small molecule with a molecular mass of 146.21 g/mol is synthesized in nerve terminals from choline and acetyl-CoA by the enzyme choline acetyltransferase. This target is widely expressed across both the central and peripheral nervous systems and its availability and uptake in nerve cells are important for maintaining optimal levels of acetylcholine.
Biological function summary

Choline and its derivative acetylcholine play important roles in cell membrane integrity and neurotransmission. Acetylcholine a constituent of the cholinergic system functions as a neurotransmitter involved in muscle activation cognitive processes and modulation of arousal. It does not typically form part of a larger complex but works in synaptic clefts to transmit signals between neurons. The presence of acetylcholine depends on the availability of choline the substrate used for its synthesis.

Pathways

Choline and acetylcholine are integral to the cholinergic signaling pathway. Acetylcholine interacts with muscarinic and nicotinic receptors to transmit neural signals. These receptors are vital parts of the pathway that modulates parasympathetic nervous system responses. Choline kinase involved in phosphorylating choline to phosphocholine links the choline metabolism pathway to cell signaling and fatty acid biosynthesis.

Choline and acetylcholine are associated with conditions like Alzheimer's disease and myasthenia gravis. Alzheimer's disease involves decreased levels of acetylcholine due to cholinergic neuron degeneration impacting cognitive functions. In myasthenia gravis an autoimmune disorder antibodies target acetylcholine receptors leading to muscle weakness. Both conditions highlight the importance of acetylcholine's relation to choline acetyltransferase as this enzyme's activity is pivotal for maintaining acetylcholine levels essential for normal nervous system function.
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Product protocols

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Target data

See full target information Choline
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Publications (70)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 11:e42673 PubMed40034321

2025

Endogenous extraction yielded high quality sulforaphane from broccoli sprouts unveils potent antioxidant and anti-Alzheimer's activities.

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Shyam Kokkattunivarthil Uthaman,Wan Seok Kang,Ju-Young Park,Sunoh Kim,Duc Dat Le,Suk-Jung Oh,Karthik Murugesh,Laura Minju Oh,Mina Lee,Jin Woo Park

International journal of molecular sciences 26: PubMed39941068

2025

CNEURO-201, an Anti-amyloidogenic Agent and σ1-Receptor Agonist, Improves Cognition in the Mouse Model of Alzheimer's Disease by Multiple Actions in the Pathology.

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Humberto Martínez-Orozco,Alberto Bencomo-Martínez,Juan Pablo Maya-Arteaga,Pedro Francisco Rubio-De Anda,Fausto Sanabria-Romero,Zyanya Gloria Mena Casas,Isaac Rodríguez-Vargas,Ana Gabriela Hernández-Puga,Marquiza Sablón-Carrazana,Roberto Menéndez-Soto Del Valle,Chryslaine Rodríguez-Tanty,Sofía Díaz-Cintra

RSC advances 14:40267-40286 PubMed39717818

2024

Cognitive-enhancing effect of fruit on scopolamine-induced cognitive impairment in rats: metabolite profiling, , and investigations.

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Hagar M Hussein,Mostafa A Abdel Kawy,Basma M Eltanany,Laura Pont,Fernando Benavente,Ahmed M Fayez,Radwan Alnajjar,Ahmed A Al-Karmalawy,Azza R Abdelmonem,Engy Mohsen

Nutrients 16: PubMed39519611

2024

Fermented Gold Kiwi Improves Gastrointestinal Motility and Functional Constipation: An Animal Study and Human Randomized Clinical Test.

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Jihye Choi,Hwal Choi,Yuseong Jang,Hyeon-Gi Paik,Hyuck-Se Kwon,Seon Mi Shin,Jeung Seung Lee,Bumseok Kim,Jungkee Kwon

Clinics (Sao Paulo, Brazil) 79:100485 PubMed39316895

2024

Exploration of the mechanism underlying the therapeutic effect of electroacupuncture at chengshan acupoint on post-hemorrhoidectomy anal pain: Insights from the mAChRs/IP3-Ca-CaM signaling pathway.

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Yang Song,Yang Wang,Ming Li,Yujuan Wang,Tianshu Xu

Cell research 34:245-257 PubMed38302740

2024

MFSD7c functions as a transporter of choline at the blood-brain barrier.

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Xuan Thi Anh Nguyen,Thanh Nha Uyen Le,Toan Q Nguyen,Hoa Thi Thuy Ha,Anna Artati,Nancy C P Leong,Dat T Nguyen,Pei Yen Lim,Adelia Vicanatalita Susanto,Qianhui Huang,Ling Fam,Lo Ngah Leong,Isabelle Bonne,Angela Lee,Jorge L Granadillo,Catherine Gooch,Dejie Yu,Hua Huang,Tuck Wah Soong,Matthew Wook Chang,Markus R Wenk,Jerzy Adamski,Amaury Cazenave-Gassiot,Long N Nguyen

Journal of molecular histology 55:51-67 PubMed38165566

2024

VNS improves VSMC metabolism and arteriogenesis in infarcted hearts through m/n-AChR-Akt-SDF-1α in adult male rats.

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Xing-Yuan Li,Jia-Qi Liu,Yan Wang,Yan Chen,Wen-Hui Hu,Yan-Xia Lv,Yan Wu,Jing Lv,Jun-Ming Tang,Deying Kong

Cellular and molecular life sciences : CMLS 81:3 PubMed38055060

2023

Mfsd7b facilitates choline transport and missense mutations affect choline transport function.

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Species

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Hoa Thi Thuy Ha,Viresh Krishnan Sukumar,Jonathan Wei Bao Chua,Dat T Nguyen,Toan Q Nguyen,Lina Hsiu Kim Lim,Amaury Cazenave-Gassiot,Long N Nguyen

ESC heart failure 10:3311-3329 PubMed37641543

2023

Vagus nerve stimulation-induced stromal cell-derived factor-l alpha participates in angiogenesis and repair of infarcted hearts.

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Yan Wang,Yun Liu,Xing-Yuan Li,Lu-Yuan Yao,MagdaleenaNaemi Mbadhi,Shao-Juan Chen,Yan-Xia Lv,Xin Bao,Long Chen,Shi-You Chen,Jing-Xuan Zhang,Yan Wu,Jing Lv,Liu-Liu Shi,Jun-Ming Tang

Heliyon 9:e17434 PubMed37426799

2023

Non-neuronal cholinergic system delays cardiac remodelling in type 1 diabetes.

Applications

Unspecified application

Species

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Pujika Emani Munasinghe,Eng Leng Saw,Matthew Reily-Bell,Devin Tonkin,Yoshihiko Kakinuma,Martin Fronius,Rajesh Katare
View all publications
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Product promise

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