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Functional Studies - Choline Assay Kit (AB219944)
Typical Choline standard calibration curve. Choline dose response was measured on a 96 well solid black plate using a Gemini fluorescence microplate reader (Molecular Devices). As low as 40nM (4 picomole/well) of choline can be detected with 30 minutes incubation time (n=3).
Product details
Total Choline Detection Kit (ab219944) provides a sensitive fluorescence-based assay for quantifying choline in cell and tissue lysates and biological fluids. The amount of choline is proportional to the concentration of hydrogen peroxide formed in the choline oxidase-mediated enzyme coupling reaction cycle, which in turn is detected with our proprietary Red Indicator. The increase in fluorescence can be easily detected at an optimal Ex/Em = 540/590 nm. The signal can also be read by absorbance at OD: 576 ± 5 nm, although the sensitivity of the assay is reduced 10-fold.
The assay detects as little as 4 picomole choline in 100 uL assay volume (40 nM), making it one of the most sensitive assays available in the market.
This product was previously known as Total Choline Detection Kit.
Other Notes
Choline is an essential nutrient. Choline and its metabolites play an important role in the structural integrity and signaling of cell membranes and cholinergic neurotransmission (choline synthesis). It is a major source of methyl group via its metabolite, trimethylglycine that participates in the S-adenosylmethionine synthesis pathways. Choline deficiency may cause liver disease, atherosclerosis and possibly neurological disorders. Despite its importance in the central nervous system as a precursor for acetylcholine and membrane phosphatidylcholine, the role of choline in mental illness has been little studied.
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Supplementary information
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Biological function summary
Choline facilitates several critical cellular functions including neurotransmitter synthesis where it is a precursor to the neurotransmitter acetylcholine. Choline does not act alone; it functions as part of complexes with phospholipids for efficient cell membrane integrity and fluidity. Choline's presence in cell membranes supports structure and aids in signal transmission and lipid transport.
Pathways
Choline occupies an essential position in the methylation and phospholipid synthesis pathways. These pathways involve enzymes such as choline kinase and phosphatidylethanolamine N-methyltransferase. Choline works closely with amino acids like methionine in methyl metabolism and it interacts with proteins like SAM (S-adenosylmethionine) in these biochemical processes impacting DNA synthesis and repair through its role as a methyl-group donor.
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Publications (3)
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Acta neuropathologica 146:565-583 PubMed37548694
2023
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Aging cell 22:e13775 PubMed36642814
2023
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Frontiers in nutrition 9:945622 PubMed35903454
2022
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