The Chromatin Condensation Assay Kit (ab139479) provides a convenient approach for analysis of late stage apoptosis by flow cytometry and microscopy, and it can also be used in microplate assays.
Images
Key facts
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Cell-based
- Reactive species
- Mammals
What's included?
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The Chromatin Condensation Assay Kit (ab139479) provides a convenient approach for analysis of late stage apoptosis by flow cytometry and microscopy, and it can also be used in microplate assays.
Key facts
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Cell-based
- Reactive species
- Mammals
- Assay Platform
- Microplate reader, Fluor. microscope, Flow cyt.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- -80°C
Notes
The Chromatin Condensation Assay Kit (ab139479) provides a convenient approach for analysis of late stage apoptosis by flow cytometry and microscopy, and it can also be used in microplate assays. The basis of the assay is that the compacted chromatin of apoptotic cells binds higher amounts of nuclear dye compared to the healthy cells. The kit is suitable for differentiating between healthy and apoptotic cells with condensed nuclei.
A control apoptosis inducing agent, Staurosporine, is provided for monitoring apoptotic changes in nuclear organization. Potential applications for live-cell studies include quickly testing cell cultures for overall health, as well as rapid screening for compounds that induce apoptosis and chromatin condensation.
The cell-permeable dye used in this application is an aromatic, planar cationic structure that inserts between stacked base pairs on the DNA duplex, providing an environmentally-dependent fluorescence enhancement of the dye molecules and large increases in fluorescence signal relative to the free dye in solution. Since the signal enhancement provides a proportional response, direct quantitation of DNA is possible. Further signal increase is observed upon DNA condensation during apoptosis. Considering the general mutagenic effect of nucleic acid-binding dyes, careful storage and handling of this dye is recommended.
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3 product images
Flow Cytometry - Chromatin Condensation Assay Kit (ab139479)
Flow cytometry analysis of Control and Staurosporine treated Jurkat cells. Raw data is presented in panels A and B where suggested gating is indicated by rectangles drawn around the cell populations. Panels C and D represent the separation of healthy and apoptotic nuclei based on their characteristic fluorescence.
Flow Cytometry - Chromatin Condensation Assay Kit (ab139479)
Flow cytometric analysis. Detection of the Sub-G0 phase in permeabilized cells using Green Cell Cycle Detection dye. In the permeabilized cells, the dye intercalates with the chromosomal DNA, resulting in a highly fluorescent healthy cell population. Upon Staurosporine treatment, a population with lower fluorescence, corresponding to fragmented DNA, can be detected via flow cytometry.
Fluorescence Microscopy - Chromatin Condensation Assay Kit (ab139479)
Absorption and fluorescence emission spectra for Green Cell Cycle Detection dye. Spectra were determined in 1X Assay Buffer.
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